Abstract
This work was focused on distinguishing the contribution of mitochondrial redox complexesto the production of reactive oxygen species (ROS) during cellular respiration. We were ableto accurately measure, for the first time, the basal production of ROS under uncoupled conditionsby using a very sensitive method, based on the fluorescent probe dichlorodihydrofluoresceindiacetate. The method also enabled the detection of the ROS generated by the oxidation ofthe endogenous substrates in the mitochondrial preparations and could be applied to bothmitochondria and live cells. Contrary to the commonly accepted view that complex III(ubiquinol:cytochrome c reductase) is the major contributor to mitochondrial ROS production, wefound that complex I (NADH-ubiquinone reductase) and complex II (succinate-ubiquinonereductase) are the predominant generators of ROS during prolonged respiration under uncoupledconditions. Complex II, in particular, appears to contribute to the basal production of ROSin cells.
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McLennan, H.R., Esposti, M.D. The Contribution of Mitochondrial Respiratory Complexes to the Production of Reactive Oxygen Species. J Bioenerg Biomembr 32, 153–162 (2000). https://doi.org/10.1023/A:1005507913372
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DOI: https://doi.org/10.1023/A:1005507913372