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Structural–Functional Analysis of Bacteriophage T7 RNA Polymerase

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Abstract

This review summarizes our results of the structural and functional studies of bacteriophage T7 DNA-dependent RNA polymerase (T7 RNAP). Particular features of this enzyme (the single-subunit composition, relatively low molecular weight) make it the most convenient model for investigating the physicochemical aspects of transcription. The review discusses the main properties of T7 RNAP, interaction between the enzyme and promoter, principle stages of T7-transcription, and also the results of structural and functional studies by affinity modification and both random and site-directed mutagenesis techniques.

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REFERENCES

  1. McAllister, W. T., and Raskin, C. A. (1993) Mol. Microbiol., 10, 1–6.

    Google Scholar 

  2. Sousa, R. (1996) Trends. Biochem. Sci., 21, 186–190.

    Google Scholar 

  3. Cermakian, N., Ikeda, T. M., Cedergren, R., and Gray, M. W. (1996) Nucleic Acids Res., 24, 648–654.

    Google Scholar 

  4. Chamberlin, M., McGrath, J., and Waskell, L. (1970) Nature, 228, 227–231.

    Google Scholar 

  5. Moffatt, B. A., Dunn, J. J., and Studier, F. W. (1984) J. Mol. Biol., 173, 265–269.

    Google Scholar 

  6. Sousa, R., Chung, Y. T., Rose, J. P., and Wang, B.–C. (1993) Nature, 364, 593–599.

    Google Scholar 

  7. Jeruzalmi, D., and Steitz, T. A. (1998) EMBO J., 17, 4101–4113.

    Google Scholar 

  8. Cheetham, G. M. T., Jeruzalmi, D., and Steitz, T. A. (1999) Nature, 399, 80–83.

    Google Scholar 

  9. Cheetham, G. M. T., and Steitz, T. A. (1999) Science, 286, 2305–2309.

    Google Scholar 

  10. Cheetham, G. M. T., and Steitz, T. A. (2000) Curr. Opin. Struct. Biol., 10, 117–123.

    Google Scholar 

  11. Steitz, T. A. (1993) Curr. Opin. Struct. Biol., 3, 31–38.

    Google Scholar 

  12. Joyce, C. M., and Steitz, T. A. (1994) Annu. Rev. Biochem., 63, 777–822.

    Google Scholar 

  13. Delarue, M., Poch, O., Tordo, N., Moras, D., and Argos, P. (1990) Protein Eng., 3, 461–467.

    Google Scholar 

  14. Tunitskaya, V. L., Luchin, S. V., Memelova, L. V., Lyakhov, D. L., Rechinsky, V. O., and Kochetkov, S. N. (1989) Mol. Biol. (Moscow), 23, 1273–1278.

    Google Scholar 

  15. Tunitskaya, V. L., Mishin, A. A., Tyurkin, V. V., Lyakhov, D. L., Rechinsky, V. O., and Kochetkov, S. N. (1988) Mol. Biol. (Moscow), 22, 1642–1649.

    Google Scholar 

  16. Rusakova, E. E., Tunitskaya, V. L., and Kochetkov, S. N. (1999) Mol. Biol. (Moscow), 33, 353–367.

    Google Scholar 

  17. Pozhitkov, A. E., Lavrik, I. N., Sergeev, M. M., and Kochetkov, S. N. (1998) Mol. Biol. (Moscow), 32, 93–97.

    Google Scholar 

  18. Lyakhov, D. L., Ilgenfritz, H., Chernov, B. K., Dragan, S. M., Rechinsky, V. O., Pokholok, D. K., Tunitskaya, V. L., and Kochetkov, S. N. (1992) Mol. Biol. (Moscow), 26, 1022–1035.

    Google Scholar 

  19. Protasevich, I. I., Memelova, L. V., Kochetkov, S. N., and Makarov, A. A. (1994) FEBS Lett., 349, 429–432.

    Google Scholar 

  20. Ikeda, R. A., and Richardson, C. C. (1987) J. Biol. Chem., 262, 3790–3799.

    Google Scholar 

  21. Martin, C. T., Muller, D. K., and Coleman, J. E. (1988) Biochemistry, 27, 3966–3974.

    Google Scholar 

  22. Muller, D. K., Martin, C. T., and Coleman, J. E. (1988) Biochemistry, 27, 5763–5771.

    Google Scholar 

  23. Tunitskaya, V. L., Akbarov, A. Kh., Luchin, S. V., Memelova, L. V., Rechinsky, V. O., and Kochetkov, S. N. (1990) Eur. J. Biochem., 191, 99–103.

    Google Scholar 

  24. Akbarov, A. Kh., Tunitskaya, V. L., and Kochetkov, S. N. (1990) Biochem. Int., 20, 1033–1040.

    Google Scholar 

  25. Lyakhov, D. L., Ilgenfritz, H., Rechinsky, V. O., Tunitskaya, V. L., Chernov, B. K., and Kochetkov, S. N. (1991) Dokl. Akad. Nauk SSSR, 317, 1005–1008.

    Google Scholar 

  26. Schenborn, E. T., and Mierendorf, R. C., Jr. (1985) Nucleic Acids Res., 13, 6223–6236.

    Google Scholar 

  27. Filippova, S. E., Ivanovskaya, M. G., Romanova, E. A., Tunitskaya, V. L., and Kochetkov, S. N. (2002) Mol. Biol. (Moscow), in press.

  28. Rechinsky, V. O., Kostyuk, D. A., Lyakhov, D. L., and Kochetkov, S. N. (1991) Mol. Biol. (Moscow), 25, 1588–1593.

    Google Scholar 

  29. Rechinsky, V. O., Kostyuk, D. A., Lyakhov, D. L., Chernov, B. K., and Kochetkov, S. N. (1993) Mol. Gen. Genet., 238, 455–458.

    Google Scholar 

  30. Rechinsky, V. O., Tunitskaya, V. L., Dragan, S. M., Kostyuk, D. A., and Kochetkov, S. N. (1993) FEBS Lett., 320, 9–12.

    Google Scholar 

  31. Rechinsky, V. O., Chernov, B. K., Dragan, S. M., Kostyuk, D. A., Tunitskaya, V. L., and Kochetkov, S. N. (1995) Mol. Gen. Genet., 247, 110–113.

    Google Scholar 

  32. Tabor, S., and Richardson, C. C. (1995) Proc. Natl. Acad. Sci. USA, 92, 6339–6343.

    Google Scholar 

  33. Rusakova, E. E., Yankin, A. P., Memelova, L. V., Tunitskaya, V. L., and Kochetkov, S. N. (1999) Mol. Biol. (Moscow), 33, 598–602.

    Google Scholar 

  34. Grachev, M. A., Zaychikov, E. E., Lukhtanov, E. A., Maksimova, T. G., and Mustayev, A. A. (1987) Bioorg. Khim., 13, 568–570.

    Google Scholar 

  35. Maksimova, T. G., Mustayev, A. A., Zaychikov, E. F., Lyakhov, D. L., Tunitskaya, V. L., Akbarov, A. Kh., Luchin, S. V., Rechinsky, V. O., Chernov, B. K., and Kochetkov, S. N. (1991) Eur. J. Biochem., 195, 841–847.

    Google Scholar 

  36. Tunitskaya, V. L., Bazhulina, N. P., Dragan, S. M., Kuznetsova, N. V., Lyakhov, D. L., Rechinsky, V. O., Morozov, Yu. V., and Kochetkov, S. N. (1993) Mol. Biol. (Moscow), 27, 51–57.

    Google Scholar 

  37. Tunitskaya, V. L., Dragan, S. M., Kostuyk, D. A., Lyakhov, D. L., Memelova, L. V., Rechinsky, V. O., and Kochetkov, S. N. (1994) Biochemistry (Moscow), 59, 355–362.

    Google Scholar 

  38. Bonner, G., Patra, D., Lafer, E. M., and Sousa, R. (1992) EMBO J., 11, 3767–3775.

    Google Scholar 

  39. Osumi–Davis, P. A., de Aguilera, M. C., Woody, R. W., and Woody, A.–Y. M. (1992) J. Mol. Biol., 226, 37–45.

    Google Scholar 

  40. Kochetkov, S. N., Rechinsky, V. O., Tunitskaya, V. L., Kostyuk, D. A., Lyakhov, D. L., and Memelova, L. V. (1994) in Modern Enzymology: Problems and Trends, Nova Science Publ. Inc., New York, pp. 277–292.

    Google Scholar 

  41. Kochetkov, S. N., Kostyuk, D. A., Lyakhov, D. L., Memelova, L. V., Rechinsky, V. O., and Tuniyskaya, V. L. (1996) in Chemical Modification of Enzymes (Kurganov, B. I., Nagradova, N. K., and Lavrik, O. I., eds.) Nova Science Publ. Inc., New York, pp. 347–387.

    Google Scholar 

  42. Tunitskaya, V. L., Rusakova, E. E., Memelova, L. V., Kochetkov, S. N., van Aershot, A., Herdewijn, P., Efimtseva, E. V., Ermolinsky, B. S., and Mikhailov, S. N. (1999) FEBS Lett., 442, 20–24.

    Google Scholar 

  43. Rusakova, E. E., Tunitskaya, V. L., Memelova, L. V., Ermolinsky, B. S., Kochetkov, S. N., and Mikhailov, S. N. (1999) Nucleosides and Nucleotides, 18, 1359–1360.

    Google Scholar 

  44. Sousa, R., and Padilla, R. (1995) EMBO J., 14, 4609–4621.

    Google Scholar 

  45. Tunitskaya, V. L., Memelova, L. V., Kostuyk, D. A., Gudima, S. O., and Kochetkov, S. N. (1997) Mol. Biol. (Moscow), 31, 365–370.

    Google Scholar 

  46. Kostuyk, D. A., Dragan, S. M., Rechinsky, V. O., Tunitskaya, V. L., Chernov, B. K., and Kochetkov, S. N. (1995) Dokl. Akad. Nauk, 346, 824–827.

    Google Scholar 

  47. Kostyuk, D. A., Dragan, S. M., Lyakhov, D. L., Rechinsky, V. O., Tunitskaya, V. L., Chernov, B. K., and Kochetkov, S. N. (1995) FEBS Lett., 369, 165–168.

    Google Scholar 

  48. Gudima, S. O., Kostyuk, D. A., Grishchenko, O. I., Tunitskaya, V. L., Memelova, L. V., and Kochetkov, S. N. (1998) FEBS Lett., 439, 302–306.

    Google Scholar 

  49. Gudima, S. O., Kazantseva, E. G., Kostyuk, D. A., Shchaveleva, I. L., Grishchenko, O. I., Memelova, L. V., and Kochetkov, S. N. (1997) Nucleic Acids Res., 25, 4614–4618.

    Google Scholar 

  50. Aphasizhev, R. A., Theobald–Dietrich, A., Kostyuk, D. A., Kochetkov, S. N., Kisselev, L. L., Giege, R., and Fasiolo, F. (1997) RNA, 3, 893–904.

    Google Scholar 

  51. Rusakova, E. E., Tunitskaya, V. L., Memelova, L. V., Kochetkova, S. V., Kostyuk, D. A., and Kochetkov, S. N. (1998) FEBS Lett., 423, 189–192.

    Google Scholar 

  52. Osumi–Davis, P. A., Sreerama, N., Volkin, D. B., Middaugh, R. C., Woody, R. W., and Woody, A.–Y. M. (1994) J. Mol. Biol., 237, 5–19.

    Google Scholar 

  53. Tunitskaya, V. L., Kochetkova, S. V., Godovikova, T. S., and Kochetkov, S. N. (2000) Mol. Biol. (Moscow), 34, 60–66.

    Google Scholar 

  54. Tunitskaya, V. L., Memelova, L. V., Skoblov, Yu. S., and Kochetkov, S. N. (2003) Mol. Biol. (Moscow), in press.

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  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 119991, Russia

    V. L. Tunitskaya & S. N. Kochetkov

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  1. V. L. Tunitskaya
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  2. S. N. Kochetkov
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Tunitskaya, V.L., Kochetkov, S.N. Structural–Functional Analysis of Bacteriophage T7 RNA Polymerase. Biochemistry (Moscow) 67, 1124–1135 (2002). https://doi.org/10.1023/A:1020911223250

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