Skip to main content
SpringerLink
Log in

Role of Double-Stranded RNA in Eukaryotic Gene Silencing

  • Published:
Molecular Biology Aims and scope Submit manuscript

Abstract

Data on RNA interference, that is, posttranscriptional gene silencing by homologous double-stranded (ds) RNA, are reviewed. Gene silencing caused by exogenous dsRNA in artificial systems and observed in transgenic organisms carrying additional gene copies is considered. Data are summarized on the mechanism that arose during evolution of the Drosophila melanogaster genome to suppress repetitive genes with the use of dsRNA and thereby to prevent male sterility. The role of dsRNA in inhibiting expression and transposition of mobile elements is discussed on the basis of authors' own and published findings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Fire, A., Xu, S., Montgomery, M.K., et al., Nature, 1998, vol. 391, pp. 806-811.

    Google Scholar 

  2. Fraser, A.G., Kamath, R.S., Zipperlen, P., et al., Nature, 2000, vol. 408, pp. 325-330.

    Google Scholar 

  3. Gonczy, P., Echeverri, G., Oegema, K., et al., Nature, 2000, vol. 408, pp. 331-336.

    Google Scholar 

  4. Montgomery, M.K., Xu, S., and Fire, A., Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 15502-15507.

    Google Scholar 

  5. Matzke, M., Matzke, A.J., and Kooter, J.M., Science, 2001, vol. 293, pp. 1080-1083.

    Google Scholar 

  6. Tuschl, T., Zamore, P.D., Lehmann, R., et al., Genes Dev., 1999, vol. 13, pp. 3191-3197.

    Google Scholar 

  7. Sharp, P.A., Genes Dev., 2001, vol. 15, pp. 485-490.

    Google Scholar 

  8. Bartel, D.B., The RNA World, Gestland, R.F., Ceck, T.R., and Atkans, J.F., Eds., Cold Spring Harbor, NY: Cold Spring Harbor Lab. Press, 1999, 2nd ed., pp. 143-162.

    Google Scholar 

  9. Eckstein, F., Aurup, H., Benseler, F., et al., Nucleic Acids Symp. Ser., 1993, vol. 29, pp. 115.

    Google Scholar 

  10. Izant, Y. and Weintraub, H., Cell, 1984, vol. 36, pp. 1007-1015.

    Google Scholar 

  11. Adoutte, A., Nature, 2000, vol. 408, pp. 37-38.

    Google Scholar 

  12. Kennerdell, J.R. and Carthew, R.W., Cell, 1998, vol. 95, pp. 1017-1026.

    Google Scholar 

  13. Sanchez Alvarado, A. and Newmark, P.A., Proc. Natl. Acad. Sci. USA, 1999, vol. 96, pp. 5049-5054.

    Google Scholar 

  14. Lohmann, J.U., Endl, I., and Bosch, T.C., Dev. Biol., 1999, vol. 214, pp. 211-214.

    Google Scholar 

  15. Ngo, H., Tschudi, C., Gull, K., and Ullu, E., Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 14687-14692.

    Google Scholar 

  16. Chuang, C.F. and Meyerowitz, E.M., Proc. Natl. Acad. Sci. USA., 2000, vol. 97, pp. 4985-4990.

    Google Scholar 

  17. Li, Y.X., Farrell, M.J., Liu, R., et al., Dev. Biol., 2000, vol. 217, pp. 394-405.

    Google Scholar 

  18. Wianny, F. and Zernicka-Goetz, M., Nat. Cell Biol., 2000, vol. 2, pp. 70-75.

    Google Scholar 

  19. Kennerdell, J.R. and Carthew, R.W., Nat. Biotechnol., 2000, vol. 18, pp. 896-898.

    Google Scholar 

  20. Lam, G. and Thummel, C.S., Curr. Biol., 2000, vol. 10, pp. 957-963.

    Google Scholar 

  21. Tavernarakis, N., Wang, S.L., Dorovkov, M., et al., Nat. Genet., 2000, vol. 24, pp. 180-183.

    Google Scholar 

  22. Tabara, H., Sarkissian, M., Kelly, W.G., et al., Cell, 1999, vol. 99, pp. 123-132.

    Google Scholar 

  23. Yang, D., Lu, H., and Erickson, J.W., Curr. Biol., 2000, vol. 10, pp. 1191-1200.

    Google Scholar 

  24. Zorio, D.A., Cheng, N.N., Blumenthal, T., and Spieth, J., Nature, 1994, vol. 372, pp. 270-272.

    Google Scholar 

  25. van der Krol, A.R., Mur, L.A., Beld, M., et al., Plant Cell, 1990, vol. 2, pp. 291-299.

    Google Scholar 

  26. Que, Q., Wang, H.-Y., English, J.J., and Jorgensen, R.A., Plant Cell, 1997, vol. 9, pp. 1357-1368.

    Google Scholar 

  27. Smith, C.J., Watson, C.F., Bird, C.R., et al., Mol. Gen. Genet., 1990, vol. 224, pp. 477-481.

    Google Scholar 

  28. Cogoni, C. and Macino, G., Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 10233-10238.

    Google Scholar 

  29. Napoli, C., Lemieux, C., and Jorgensen, R., Plant Cell, 1990, vol. 2, pp. 279-289.

    Google Scholar 

  30. Holtorf, H., Schob, H., Kunz, C., et al., Plant Cell, 1999, vol. 11, pp. 471-484.

    Google Scholar 

  31. van Eldik, G.J., Litiere, K., Jacobs, J.J., et al., Nucleic Acids Res., 1998, vol. 26, pp. 5176-5181.

    Google Scholar 

  32. Sijen, T. and Kooter, J.M., BioEssays, 2000, vol. 22, pp. 520-531.

    Google Scholar 

  33. Schiebel, W., Haas, B., Marinkovic, S., et al., J. Biol. Chem., 1993, vol. 268, pp. 11851-11857.

    Google Scholar 

  34. Dalmay, T., Hamilton, A., Rudd, S., et al., Cell, 2000, vol. 101, pp. 543-553.

    Google Scholar 

  35. Mourrain, P., Beclin, C., Elmayan, T., et al., Cell, 2000, vol. 101, pp. 533-542.

    Google Scholar 

  36. Cogoni, C. and Macino, G., Nature, 1999, vol. 399, pp. 166-169.

    Google Scholar 

  37. Smith, N.A., Singh, S.P., Wang, M.B., et al., Nature, 2000, vol. 407, pp. 319-320.

    Google Scholar 

  38. Hamilton, A.J. and Baulcombe, D.C., Science, 1999, vol. 286, pp. 950-952.

    Google Scholar 

  39. Hutvagner, G., Mlynarova, L., and Nap, J.P., RNA, 2000, vol. 6, pp. 1445-1454.

    Google Scholar 

  40. Elbashir, S.M., Lendeckel, W., and Tuschl, T., Genes Dev., 2001, vol. 15, pp. 188-200.

    Google Scholar 

  41. Zamore, P.D., Tuschl, T., Sharp, P.A., and Bartel, D.P., Cell, 2000, vol. 101, pp. 25-33.

    Google Scholar 

  42. Hammond, S.M., Bernstein, E., Beach, D., and Hannon, G.J., Nature, 2000, vol. 404, pp. 293-296.

    Google Scholar 

  43. Parrish, S., Fleenor, J., Xu, S., et al., Mol. Cell, 2000, vol. 6, pp. 1077-1087.

    Google Scholar 

  44. Fierro-Monti, I. and Mathews, M.B., Trends Biochem. Sci., 2000, vol. 25, pp. 241-246.

    Google Scholar 

  45. Knight, S.W. and Bass, B.L., Science, 2001, vol. 293, pp. 2269-2271.

    Google Scholar 

  46. Bernstein, E., Caudy, A.A., Hammond, S.M., and Hannon, G.J., Nature, 2001, vol. 409, pp. 363-366.

    Google Scholar 

  47. Fagard, M., Boutet, S., Morel, J.B., et al., Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 11650-11654.

    Google Scholar 

  48. Cerutti, L., Mian, N., and Bateman, A., Trends Biochem. Sci., 2000, vol. 25, pp. 481-482.

    Google Scholar 

  49. Hammond, S.M., Boettcher, S., Caudy, A.A., et al., Science, 2001, vol. 293, pp. 1146-1150.

    Google Scholar 

  50. Hutvagner, G., McLachlan, J., Pasquinelli, A.E., et al., Science, 2001, vol. 293, pp. 834-838.

    Google Scholar 

  51. Pasquinelli, A.E., Reinhart, B.J., Slack, F., et al., Nature, 2000, vol. 208, pp. 86-89.

    Google Scholar 

  52. Brigneti, G., Voinnet, O., Li, W.X., et al., EMBO J., 1998, vol. 17, pp. 6739-6746.

    Google Scholar 

  53. Anandalakshmi, R., Marathe, R., Ge, X., et al., Science, 2000, vol. 290, pp. 142-144.

    Google Scholar 

  54. Mallory, A.C., Ely, L., Smith, T.H., et al., Plant Cell, 2001, vol. 13, pp. 571-583.

    Google Scholar 

  55. Aravin, A.A., Naumova, N.M., Tulin, A.V., et al., Curr. Biol., 2001, vol. 11, pp. 1017-1027.

    Google Scholar 

  56. Livak, K., Genetics, 1984, vol. 107, pp.611-634

    Google Scholar 

  57. Livak, K., Genetics, 1990, vol. 124, pp. 303-316.

    Google Scholar 

  58. Nurminsky, D.I., Shevelyov, Y., Nuzhdin, S.V., and Gvozdev, V.A., Chromosoma, 1994, vol. 103, pp. 277-285.

    Google Scholar 

  59. Tulin, A.V., Kogan, G.L., Filipp, D., et al., Genetics, 1997, vol. 146, pp. 253-262.

    Google Scholar 

  60. Gvozdev, V.A., Alatortsev, V.E., Aravin, A.A., et al., Mol. Biol., 1999, vol. 33, pp. 14-25.

    Google Scholar 

  61. Bozzetti, M.P., Massari, S., Finelli, P., et al., Proc. Natl. Acad. Sci. USA, 1995, vol. 92, pp. 6067-6071.

    Google Scholar 

  62. Balakireva, M.D., Shevelyov, Y.Y., Nurminsky, D., et al., Nucleic Acids Res., 1992, vol. 20, pp. 3731-3736.

    Google Scholar 

  63. Danilevskaya, O.N., Kurenova, E.V., Pavlova, M.N., et al., Chromosoma, 1991, vol. 100, pp. 118-124.

    Google Scholar 

  64. Kalmykova, A., Dobritsa, A., and Gvozdev, V., FEBS Lett., 1997, vol. 416, pp. 164-166.

    Google Scholar 

  65. Kalmykova, A.I., Dobritsa, A.A., and Gvozdev, V.A., Genetics, 1998, vol. 148, pp. 243-249.

    Google Scholar 

  66. Xie, Z., Fan, B., Chen, C., and Chen, Z., Proc. Natl. Acad. Sci. USA, 2001, vol. 98, pp. 6516-6521.

    Google Scholar 

  67. Schmidt, A., Palumbo, G., Bozzetti, M.P., et al., Genetics, 1999, vol. 151, pp. 749-760.

    Google Scholar 

  68. Stapleton, W., Das, S., and McKee, B.D., Chromosoma, 2001, vol. 110, pp. 228-240.

    Google Scholar 

  69. Dalmay, T., Horsefield, R., Braunstein, T.H., and Baulcombe, D.C., EMBO J., 2001, vol. 20, pp. 2069-2078.

    Google Scholar 

  70. Domeier, M.E., Morse, D.P., Knight, S.W., et al., Science, 2000, vol. 289, pp. 1928-1931.

    Google Scholar 

  71. Wu-Scharf, D., Jeong, B., Zhang, C., and Cerutti, H., Science, 2000, vol. 290, pp. 1159-1163.

    Google Scholar 

  72. Pasyukova, E.G., Nuzhdin, S.V., Filatov, D.A., and Gvozdev, V.A., Mol. Biol., 1999, vol. 33, pp. 26-37.

    Google Scholar 

  73. Mason, J.M. and Biessmann, H., Trends Genet., 1995, vol. 11, pp. 58-62.

    Google Scholar 

  74. Ketting, R.F., Haverkamp, T.H., van Luenen, H.G., and Plasterk, R.H., Cell, 1999, vol. 99, pp. 133-141.

    Google Scholar 

  75. Ilyin, Y.V., Chmeliauskaite, V.G., and Georgiev, G.P., Nucleic Acids Res., 1980, vol. 8, pp. 3439-3457.

    Google Scholar 

  76. Minchiotti, G. and Di Nocera, P.P., Mol. Cell. Biol., 1991, vol. 11, pp. 5171-5180.

    Google Scholar 

  77. Lankenau, S., Corces, V., and Lankenau, D., Mol. Cell. Biol., 1994, vol. 14, pp. 1764-1775.

    Google Scholar 

  78. Ketting, R.F. and Plasterk, R.H., Nature, 2000, vol. 404, pp. 296-298.

    Google Scholar 

  79. Kelly, W.G. and Fire, A., Development, 1998, vol. 125, pp. 2451-2456.

    Google Scholar 

  80. Hunter, T., Hunt, T., Jackson, R.J., and Robertson, H.D., J. Biol. Chem., 1975, vol. 250, pp. 409-417.

    Google Scholar 

  81. Elbashir, S.M., Harborth, J., Lendeckel, W., et al., Nature, 2001, vol. 411, pp. 494-498.

    Google Scholar 

  82. Rossi, J.J., Chem. Biol., 1999, vol. 6, pp. 3-37.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia

    A. A. Aravin, M. S. Klenov, V. V. Vagin, Ya. M. Rozovskii & V. A. Gvozdev

Authors
  1. A. A. Aravin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. S. Klenov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. V. Vagin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ya. M. Rozovskii
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. A. Gvozdev
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aravin, A.A., Klenov, M.S., Vagin, V.V. et al. Role of Double-Stranded RNA in Eukaryotic Gene Silencing. Molecular Biology 36, 180–188 (2002). https://doi.org/10.1023/A:1015357603566

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1015357603566

Search

Navigation