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Screening wild plants of Capsicum annuum for resistance to pepper huasteco virus (PHV): Presence of viral DNA and differentiation among populations

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Abstract

Plants collected in thirteen wild populations of Capsicum annuum from Northwest Mexico were tested for resistance to the pepper huasteco begomovirus (formerly subgroup III) (PHV) that is transmitted by the white fly Bemisia tabaci Genadius. Plants were inoculated using both grafting and biolistic methods. Presence of viral DNA was detected by dot blot hybridization and densitometry. Populations varied in their resistance to PHV. Plants of only two of the populations either did not develop disease symptoms or showed very light symptoms after inoculation. In some cases, symptoms appeared several days after inoculation. In plants of these populations viral DNA was detected by dot-blot hybridization assays but they appear to be a good source of resistance (symptomless) for use in breeding programmes.

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Authors and Affiliations

  1. Facultad de Agronomía, Universidad Autónoma de Sinaloa Instituto de Ecología, Universidad Nacional Autónoma de México (UNAM), Apdo. Postal 70-275, 04510, México, D.F, México

    Sergio Hernández-Verdugo

  2. Centro de Investigaciones de Estudios Avanzados (CINVESTAV-IPN), Unidad Irapuato, Apdo. Postal 629, 36500, Irapuato, Guanajuato, México

    Ramón G. Guevara-González & Rafael F. Rivera-Bustamante

  3. Instituto de Ecología, Universidad Nacional Autónoma de México (UNAM), Apdo. Postal 70-275, 04510, México, D.F, México

    Ken Oyama

Authors
  1. Sergio Hernández-Verdugo
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  2. Ramón G. Guevara-González
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  3. Rafael F. Rivera-Bustamante
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  4. Ken Oyama
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Hernández-Verdugo, S., Guevara-González, R.G., Rivera-Bustamante, R.F. et al. Screening wild plants of Capsicum annuum for resistance to pepper huasteco virus (PHV): Presence of viral DNA and differentiation among populations. Euphytica 122, 31–36 (2001). https://doi.org/10.1023/A:1012624830340

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