Abstract
The domestic dog (Canis familiaris) is widely used as a model in the study of human disease. However, many of the 78 chromosomes comprising the canine karyotype are extremely difficult to identify reliably by classical cytogenetics. This has been a major hindrance to molecular cytogenetic studies of this species. The Animal Health Trust and the Sanger Centre have developed a set of canine whole chromosome-specific fluorescence in situ hybridisation (FISH) probes (chromosome paints). We have used these chromosome paints to identify unequivocally each chromosome in a metaphase spread. An increasing number of laboratories are making use of cooled CCD cameras and sophisticated software for FISH mapping. Consequently, there is a major trend towards the use of DAPI banding for concurrent chromosome identification during FISH analyses in a range of species. Here we present, for the first time, a complete DAPI banded karyotype of the dog in which each chromosome has been accurately placed, together with a 460-band DAPI ideogram. These data will facilitate the accurate assignment of FISH- mapped loci to all chromosomes comprising the karyotype and form the basis for an agreed standard of the dog karyotype.
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Breen, M., Bullerdiek, J. & Langford, C.F. The DAPI Banded Karyotype of the Domestic Dog (Canis familiaris) Generated Using Chromosome-Specific Paint Probes. Chromosome Res 7, 401–406 (1999). https://doi.org/10.1023/A:1009224232134
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DOI: https://doi.org/10.1023/A:1009224232134