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Functional analysis of the latency-related gene of bovine herpesvirus types 1 and 5

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Abstract

Bovine herpesvirus type 1 and type 5 (BoHV-1 and BoHV-5) are two alphaherpesviruses that affect cattle with two different syndromes. While BoHV-1 mainly produces respiratory symptoms, BoHV-5 is highly neuropathogenic and responsible for meningoencephalitis in young cattle. The latency-related (LR) gene, which is not conserved between these two herpesviruses, is the only viral gene abundantly expressed in latently infected neurons. The antiapoptotic action of this gene has been demonstrated during acute infection and reactivation from latency and seems to be mainly mediated by a LR protein (ORF-2) which is truncated in amino acid 51 in the case of BoHV-5. In this work, we show that the BoHV-5 LR gene is less efficient at cell survival and apoptosis inhibition in transient as well as in established neuronal cell lines compared to its BoHV-1 homolog. We hypothesize that the BoHV-5 LR gene may have novel functions that are lacking in the BoHV-1 LR gene and that these differences may contribute to its enhanced neuropathogenesis.

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Acknowledgments

We would like to thank Fiorella Kotsias for reviewing the manuscript and providing valuable suggestions.

Funding

This study was supported by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, Argentina) PICT 2010-0925 to ACB. CS was granted a scholarship type I from ANPCyT and a type II scholarship from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).

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Author notes

  1. Clinton Jones

    Present address: Department of Veterinary Pathobiology, Oklahoma State University Center for Veterinary Health Sciences, Stillwater, OK, USA

Authors and Affiliations

  1. Facultad de Ciencias Veterinarias, Cátedra de Virología, Universidad de Buenos Aires, Av Chorroarin 280, CABA Buenos Aires, Argentina

    Carolina Silvestro & Ana Bratanich

  2. University of Nebraska Lincoln, Lincoln, NE, USA

    Clinton Jones

Authors
  1. Carolina Silvestro
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  2. Clinton Jones
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  3. Ana Bratanich
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Correspondence to Ana Bratanich.

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Silvestro, C., Jones, C. & Bratanich, A. Functional analysis of the latency-related gene of bovine herpesvirus types 1 and 5. J. Neurovirol. 25, 597–604 (2019). https://doi.org/10.1007/s13365-019-00745-y

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  • DOI: https://doi.org/10.1007/s13365-019-00745-y

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