Abstract
Cutaneous leishmaniasis is one of the important skin diseases with diverse clinical manifestations. With an incidence of 0.7–1.3 million cases annually, this disease is often reported from six countries, including Iran. Accordingly, the purpose of this study was to evaluate the anti-leishmanial effect of the three plant hydroalcoholic extracts including fleawort (Plantago psyllium L.), savory (Satureja hortensis L.) and tarragon (Artemisia dracunculus L.) on Leishmania major promastigotes. The hydroalcoholic extract from each plant was extracted and its anti-leishmanial effect was evaluated in different concentrations (100–1000 µg/ml) and at various hours (24, 48 and 72 h). Savory herb inhibitory concentration 50 % (IC50) at 24, 48 and 72 h was 790.81, 398.11 and 298.42 µg/ml, respectively. In addition, tarragon herb IC50 at 24, 48 and 72 h was 962.03, 688.36 and 585.51 µg/ml, respectively. Moreover, the fleawort extract was showed the lowest effect, considering that its effect at the concentration of 1000 µg/ml was 48 % after 72 h (P > 0.05). Furthermore, the statistical analysis showed a significant difference for interaction between concentration and time regarding the tarragon and savory extracts with a P value of lower than 0.05. According to the results, the anti-leishmanial effect of the tarragon and savory extracts may make it possible to use them in the treatment of cutaneous leishmaniasis as a complementary or alternative therapy; however, further studies are necessary and should be evaluated in cell culture and in vivo conditions to confirm it.
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References
Arias AR, Pandolfi E, Vega MC, Rolon M (2012) Selected natural and synthetic phenolic compounds with antileishmanial activity: a five-year review. Curr Bioact Compd 8:307–333
Azizi K, Shahidi-Hakak F, Asgari Q, Hatam GR, Fakoorziba MR, Miri R, Djaefar Moemenbellah-Fard M (2014) In vitro efficacy of ethanolic extract of Artemisia absinthium (Asteraceae) against Leishmania major L. using cell sensitivity and flow cytometry assays. J Parasit Dis. doi:10.1007/s12639-014-0569-5
Croft SL, Sundar S, Fairlamb AH (2006) Drug resistance in leishmaniasis. Clin Microbiol Rev 19:111–126
Cruz-Ede M, da Silva ER, Maquiaveli-Cdo C, Alves ES, Lucon JF Jr, dos Reis MB, de Toledo CE, Cruz FG, Vannier-Santos MA (2013) Leishmanicidal activity of Cecropia pachystachya flavonoids: arginase inhibition and altered mitochondrial DNA arrangement. Phytochemistry 89:71–77
Dutta A, Ghoshal A, Mandal D, Mondal NB, Banerjee S, Sahu NP, Mandal C (2007) Racemoside A, an anti-leishmanial, water–soluble, natural steroidal saponin, induces programmed cell death in Leishmania donovani. J Med Microbiol 56:1196–1204
Fata A, Salehi Sangani G, Rafatpanah H, Mousavi Bazzaz M, Mohaghegh MA, Movahedi A (2015) Identification of Leishmania species by kinetoplast DNA-polymerase chain reaction for the first time in Khaf district, Khorasan-e-Razavi province, Iran. Trop Parasitol 5:50–54
Hooshyar H, Talari S, Feyzi F (2014) Therapeutic effect of Hedera helix alcoholic extract against cutaneous leishmaniasis caused by Leishmania major in Balb/c mice. Jundishapur J Microbiol 7:e9432
Karami M, Doudi M, Setorki M (2013) Assessing epidemiology of cutaneous leishmaniasis in Isfahan, Iran. J Vector Borne Dis 50:30–37
Kaur A, Kaur PK, Singh S, Singh IP (2014) Antileishmanial compounds from Moringa oleifera Lam. Z Naturforsch C 69:110–116
Khoshzaban F, Ghaffarifar F, Jamshidi-Koohsari HR (2014) Peganum harmala aqueous and ethanol extracts effects on lesions caused by Leishmania major (MRHO/IR/75/ER) in BALB/c mice. Jundishapur J Microbiol 7:e10992
Mahboubi M, Kazempour N (2011) Chemical composition and antimicrobial activity of Satureja hortensis and Trachyspermum copticum essential oil. Iran J Microbiol 3:194–200
Mahmoudvand H, Ezzatkhah F, Sharififar F, Sharifi I, Dezaki ES (2015) Antileishmanial and cytotoxic effects of essential oil and methanolic extract of Myrtus communis L. Korean J Parasitol 53:21–27
McGwire BS, Satoskar AR (2014) Leishmaniasis: clinical syndromes and treatment. QJM 107:7–14
Mohammadpour G, Marzony ET, Farahmand M (2012) Evaluation of the anti-Leishmaniamajor activity of Satureja bakhtiarica essential oil in vitro. Nat Prod Commun 7:133–136
Nosratabadi SJ, Sharifi I, Sharififar F, Bamorovat M, Daneshvar H, Mirzaie M (2015) In vitro antileishmanial activity of methanolic and aqueous extracts of Eucalyptus camaldulensis against Leishmania major. J Parasit Dis 39:18–21
Obolskiy D, Pischel I, Feistel B, Glotov N, Heinrich M (2011) Artemisia dracunculus L. (tarragon): a critical review of its traditional use, chemical composition, pharmacology, and safety. J Agric Food Chem 59:11367–11384
Pan SY, Zhou SF, Gao SH, Yu ZL, Zhang SF, Tang MK, Sun JN, Ma DL, Han YF, Fong WF, Ko KM (2013) New perspectives on how to discover drugs from herbal medicines: CAM’s outstanding contribution to modern therapeutics. Evid Based Complement Altern Med 2013:627375
Peixoto JA, Andrade E, Silva ML, Crotti AE, Cassio Sola Veneziani R, Gimenez VM, Januário AH, Groppo M, Magalhães LG, Dos Santos FF, Albuquerque S, da Silva Filho AA, Cunha WR (2011) Antileishmanial activity of the hydroalcoholic extract of Miconia langsdorffii, isolated compounds, and semi-synthetic derivatives. Molecules 16:1825–1833
Rodrigues IA, Azevedo MM, Chaves FC, Alviano CS, Alviano DS, Vermelho AB (2014) Arrabidaea chica hexanic extract induces mitochondrion damage and peptidase inhibition on Leishmania spp. Biomed Res Int 2014:985171
Rodrigues KA, Amorim LV, Dias CN, Moraes DF, Carneiro SM, Carvalho FA (2015) Syzygium cumini (L.) Skeels essential oil and its major constituent α-pinene exhibit anti-Leishmania activity through immunomodulation in vitro. J Ethnopharmacol 160:32–40
Saki J, Khademvatan S, Pazyar N, Eskandari A, Tamoradi A, Nazari P (2015) In vitro activity of Cordia myxa mucilage extract against Leishmania major and L. infantum promastigotes. Jundishapur J Microbiol 8:e19640
Salehi G, Fata A, Mohaghegh MA, Mousavi-Bazzaz SM, Rafatpanah H, Movahedi A (2014) Molecular identification of Leishmania species in Taybad district, Iran. Asian Pac J Trop Dis 4:S535–S539
Santos WR, Bernardo RR, Peçanha LM, Palatnik M, Parente JP, Palatnik de Sousa CB (1997) Haemolytic activities of plant saponins and adjuvants. Effect of Periandra mediterranea saponin on the humoral response to the FML antigen of Leishmania donovani. Vaccine 15:1024–1029
Serakta M, Djerrou Z, Mansour-Djaalab H, Kahlouche-Riachi F, Hamimed S, Trifa W, Belkhiri A, Edikra N, Hamdi Pacha Y (2013) Antileishmanial activity of some plants growing in Algeria: Juglans regia, Lawsonia inermis and Salvia officinalis. Afr J Tradit Complement Altern Med 10:427–430
World Health Organization (2015) Leishmaniasis. www.who.int/mediacentre/factsheets/fs375/en. Accessed Feb 2015
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Mirzaei, F., Bafghi, A.F., Mohaghegh, M. et al. In vitro anti-leishmanial activity of Satureja hortensis and Artemisia dracunculus extracts on Leishmania major promastigotes. J Parasit Dis 40, 1571–1574 (2016). https://doi.org/10.1007/s12639-015-0730-9
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DOI: https://doi.org/10.1007/s12639-015-0730-9