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Competition/antagonism associations of biofilm formation among Staphylococcus epidermidis Agr groups I, II, and III

  • Microbial Pathogenesis and Host-Microbe Interaction
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Abstract

Staphylococci have quorum-sensing (QS) systems that enable cell-to-cell communication, as well as the regulation of numerous colonization and virulence factors. The accessory gene regulator (Agr) operon is one of the Staphylococcus genus QS systems. Three groups (I, II, and III) are present in Staphylococcus epidermidis Agr operon. To date, it is unknown whether Agr groups can interact symbiotically during biofilm development. This study analyzed a symbiotic association among Agr groups during biofilm formation in clinical and commensal isolates. Different combinations among Agr group isolates was used to study biofilm formation in vitro and in vivo (using a mouse catheter-infection model). The analysis of Agr groups were also performed from samples of human skin (head, armpits, and nostrils). Different predominant coexistence was found within biofilms, suggesting symbiosis type. In vitro, Agr I had a competition with Agr II and Agr III. Agr II had a competition with Agr III, and Agr II was an antagonist to Agr I and III when the three strains were combined. In vivo, Agr II had a competition to Agr I, but Agr I and II were antagonists to Agr III. The associations found in vitro and in vivo were also found in different sites of the skin. Besides, other associations were observed: Agr III antagonized Agr I and II, and Agr III competed with Agr I and Agr II. These results suggest that, in S. epidermidis, a symbiotic association of competition and antagonism occurs among different Agr groups during biofilm formation.

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Authors and Affiliations

  1. Laboratory of Immunomicrobiology, Department of Microbiology, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, 11340, Mexico City, Mexico

    Sergio Martínez-García, César I. Ortiz-García & Juan C. Cancino-Diaz

  2. Laboratory of Genetics, Department of Genetics-Research Unit, Instituto de Oftalmología Conde de Valenciana, 06800, Mexico City, Mexico

    Marisa Cruz-Aguilar & Juan Carlos Zenteno

  3. Laboratory of Innate Immunity, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, 11340, Mexico City, Mexico

    José Martin Murrieta-Coxca, Sandra Rodríguez-Martínez & Mario E. Cancino-Diaz

  4. “Unidad de Desarrollo e Investigación en Bioprocesos” (UDIBI), Department of Immunology, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, 11340, Mexico City, Mexico

    Sonia Mayra Pérez-Tapia

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  1. Sergio Martínez-García
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  2. César I. Ortiz-García
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  3. Marisa Cruz-Aguilar
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  8. Mario E. Cancino-Diaz
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Correspondence to Juan C. Cancino-Diaz.

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Martínez-García, S., Ortiz-García, C.I., Cruz-Aguilar, M. et al. Competition/antagonism associations of biofilm formation among Staphylococcus epidermidis Agr groups I, II, and III. J Microbiol. 57, 143–153 (2019). https://doi.org/10.1007/s12275-019-8322-5

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