Abstract
Endophytic bacteria have been found in virtually every plant studied, where they colonize the internal tissues of their host plant and can form a range of different beneficial relationships. The diversity of bacterial endophytes associated with ginseng plants of varying age levels in Korea was investigated. Fifty-one colonies were isolated from the interior of ginseng stems. Although a mixed composition of endophyte communities was recovered from ginseng based on the results of 16S rDNA analysis, bacteria of the genus Bacillus and Staphylococcus dominated in 1-year-old and 4-year-old plants, respectively. Phylogenetic analysis revealed four clusters: Firmicutes, Actinobacteria, α-Proteobacteria, and γ-Proteobacteria, with Firmicutes being predominant. To evaluate the plant growth promoting activities, 18 representative isolates were selected. Amplification of nifH gene confirmed the presence of diazotrophy in only two isolates. Half of the isolates solubilized mineral phosphate. Except four, all the other endophytic isolates produced significant amounts of indole acetic acid in nutrient broth. Iron sequestering siderophore production was detected in seven isolates. Isolates E-I-3 (Bacillus megaterium), E-I-4 (Micrococcus luteus), E-I-8 (B. cereus), and E-I-20 (Lysinibacillus fusiformis) were positive for most of the plant growth promoting traits, indicating their role in growth promotion of ginseng.
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Araújo, W.L., J. Marcon, W. Maccheroni, J.D. van Elas, Jr., J.W.L. van Vuurde, and J.L. Azevedo. 2002. Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. Appl. Environ. Microbiol. 68, 4906–4914.
Bai, Y., D.A. Frederic, L.S. Donald, and T.D. Brian. 2002. Isolation of plant-growth-promoting Bacillus strains from soybean root nodules. Can. J. Microbiol. 48, 230–238.
Burgmann, H., F. Widmer, W. Von Sigler, and J. Zeyer. 2004. New molecular screening tools for analysis of free-living diazotrophs in soil. Appl. Environ. Microbiol. 70, 240–247.
Chanway, C.P., M. Shishido, J. Nairn, S.S. Jungwirth, J. Markham, G. Xiao, and F.B. Holl. 2000. Endophytic colonization and field responses of hybrid spruce seedlings after inoculation with plant growth-promoting rhizobacteria. For. Ecol. Manag. 133, 81–88.
Chi, F., S.H. Shen, H.P. Cheng, Y.X. Jing, Y.G. Yanni, and F.B. Dazzo. 2005. Ascending migration of endophytic rhizobia from roots to leaves, inside rice plants and assessment of benefits to rice growth physiology. Appl. Environ. Microbiol. 71, 7271–7278.
Cho, K.M., S.Y. Hong, S.M. Lee, Y.H. Kim, G.G. Kahng, Y.P. Lim, H. Kim, and H.D. Yun. 2007. Endophytic bacterial communities in ginseng and their antifungal activity against pathogens. Microb. Ecol. 54, 341–351.
Cho, S.J., S.R. Park, M.K. Kim, W.J. Lim, S.K. Ryu, C.L. An, S.Y. Hong, and et al. 2002. Endophytic Bacilus sp. isolated from the interior of ballon flower root. Biosci. Biotechnol. Biochem. 66, 1270–1275.
Costacurta, A. and J. Vanderleyden. 1995. Synthesis of phytohormones by plant associated bacteria. Critical Rev. Microbiol. 21, 1–18.
Feng, Y., D. Shen, and W. Song. 2006. Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthates. J. Appl. Microbiol. 100, 938–945.
Ferreira, E.P., A.N. Dusi, G.R. Xavier, and N.G. Rumjanek. 2008. Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles. Pesq. Agropec. Bras. 43, 605–612.
Goldstein, A.H. 1986. Bacterial solubilization of mineral phosphates: historical perspective and future prospects. Am. J. Alter. Agric. 1, 51–57.
Goldstein, A.H., R.D. Rogers, and G. Mead. 1993. Separating phosphate from ores via bioprocessing. Biotechnology 11, 1250–1254.
Hartmann, A., M. Singh, and W. Klingmueller. 1983. Isolation and characterization of Azospirillum mutants excreting high amounts of indole acetic acid. Can. J. Microbiol. 29, 916–923.
Jha, P.N. and A. Kumar. 2007. Endophytic colonization of Typha australis by a plant growth-promoting bacterium Klebsiella oxytoca strain GR-3. J. Appl. Microbiol. 103, 1311–1320.
Kanvinde, L. and G.R.K. Sastry. 1990. Agrobacterium tumefaciens is a diazotrophic bacterium. Appl. Environ. Microbiol. 56, 2087–2092.
Kobayashi, D.Y. and J.D. Palumbo. 2000. Bacterial endophytes and their effects on plants and uses in agriculture, pp. 199–233. In C.W. Bacon and J.F. White (eds.), Microbial endophytes. Marcel Dekker, Inc., New York, N.Y., USA.
Lacava, P.T., M.E. Silva-Stenico, W.L. Araújo, V.C. Simionato, E. Carrilho, S.M. Tsai, and J.L. Azevedo. 2008. Detection of siderophores in endophytic bacteria Methylobacterium spp. associated with Xylella fastidiosa subsp. Pauca. Pesq. Agropec. Bras. 43, 521–528.
Lodewyck, C., J. Vangronsveld, F. Porteous, E.R.B. Moore, S. Taghavi, M. Mezgeay, and D.V.D. Lelie. 2002. Endophytic bacteria and their potential application. Crit. Rev. Plant. Sci. 86, 583–606.
McGinnis, S. and T.L Madden. 2004. BLAST: at the core of a powerful and diverse set of sequence analysis tools. Nucleic Acids Res. 32, 20–25.
Park, M.S., S.R. Jung, M.S. Lee, K.O. Kim, J.O. Do, K.H. Lee, S.B. Kim, and K.S. Bae. 2005a. Isolation and characterization of bacteria associated with two sand dune plant species, Calystegia soldanella and Elymus mollis. J. Microbiol. 43, 219–227.
Park, M., C. Kim, J. Yang, H. Lee, W. Shin, S. Kim, and T. Sa. 2005b. Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea. Microbiol. Res. 160, 127–133.
Potrich, D.P., L.M.P. Passaglia, and I.S. Schrank. 2003. Partial characterization of nif genes from the bacterium Azospirillum amazonense. Braz. J. Med. Biol. Rev. 34, 1105–1113.
Qiu, F., Y. Huang, L. Sun, X. Zhang, Z. Liu, and W. Song. 2007. Leifsonia ginsengi sp. nov., isolated from ginseng root. Int. J. Syst. Evol. Microbiol. 57, 405–408.
Ramesh, R., A.A. Joshi, and M.P. Ghanekar. 2009. Pseudomonads: major antagonistic endophytic bacteria to suppress bacterial wilt pathogen, Ralstonia solanacearum in the eggplant (Solanum melongena L). World J. Microbiol. Biotechnol. 25, 47–55.
Reinhold-Hurek, B. and T. Hurek. 1998. Life in grasses: diazotrophic endophytes. Trends Microbiol. 6, 139–144.
Schwyn, B. and J.B. Neilands. 1987. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160, 47–56.
Seghers, D., L. Wittebolle, E.M. Top, W. Verstraete, and S.D. Siciliano. 2004. Impact of agricultural practice on the Zea mays L. endophytic community. Appl. Environ. Microbiol. 70, 1475–1482.
Sharma, A. and B.N. Johri. 2003. Growth promoting influence of siderophore-producing Pseudomonas strains GRP3A and PRS9 in maize (Zea mays L.) under iron limiting conditions. Microbiol. Res. 158, 243–248.
Siciliano, S.D., N. Fortin, A. Mihoc, G. Wisse, S. Labelle, D. Beaumier, D. Ouellette, and et al. 2001. Selection of specific endophytic bacterial genotypes by plants in response to soil contamination. Appl. Environ. Microbiol. 67, 2469–2475.
Steward, G.F., B.D. Jenkins, B.B. Ward, and J.P. Zehr. 2004. Development and testing of a DNA microarray to assess nitrogenase (nifH) gene diversity. Appl. Environ. Microbiol. 70, 1455–1465.
Surette, M.A., A.V. Sturz, R.R. Lada, and J. Nowak. 2003. Bacterial endophytes in processing carrots (Daucas carota L. var. sativus): their location, population density, biodiversity and their effects on plant growth. Plant Soil 253, 381–390.
Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599.
Tompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673–4680.
Velázquez, E., M. Rojas, M.J. Lorite, R. Rivas, J.L. Zurdo-Piñeiro, M. Heydrich, and E.J. Bedmar. 2008. Genetic diversity of endophytic bacteria which could be found in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants. J. Basic Microbiol. 48, 118–124.
Yu, W.J., B.J. Lee, S.Y. Nam, D.C. Yang, and Y.W. Yun. 2003. Modulating effects of Korean ginseng saponins on ovarian function immature rats. Biol. Pharm. Bull. 26, 2574–2580.
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Vendan, R.T., Yu, Y.J., Lee, S.H. et al. Diversity of endophytic bacteria in ginseng and their potential for plant growth promotion. J Microbiol. 48, 559–565 (2010). https://doi.org/10.1007/s12275-010-0082-1
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DOI: https://doi.org/10.1007/s12275-010-0082-1