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Proteomic Investigation of Malignant Major Salivary Gland Tumors

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Abstract

The purpose of this study was to define the proteome profile of fine needle aspiration (FNA) samples of malignant major salivary gland tumors (MSGT) compared to benign counterparts, and to evaluate potential clinical correlations and future applications. Patients affected by MSGT (n = 20), pleomorphic adenoma (PA) (n = 37) and Warthin’s tumor (WT) (n = 14) were enrolled. Demographic, clinical and histopathological data were registered for all patients. FNA samples were processed to obtain the protein extracts. Protein separation was obtained by two-dimensional electrophoresis (2-DE) and proteins were identified by mass spectrometry. Western blot analysis was performed to validate the 2-DE results. Statistical differences between groups were calculated by the Mann–Whitney U test for non-normal data. Spearman’s rank correlation coefficient was calculated to evaluate correlations among suggested protein biomarkers and clinical parameters. Twelve and 27 differentially expressed spots were found for MSGT versus PA and MSGT versus WT, respectively. Among these, annexin-5, cofilin-1, peptidyl-prolyl-cis–trans-isomerase-A and F-actin-capping-alpha-1 were able to differentiate MSGT from PA, WT, and healthy samples. Moreover, STRING analysis suggested cofilin-1 as a key node of protein interactions. Some of the overexpressed proteins are related to some clinical factors of our cohort, such as survival and outcome. Our results suggest potential protein biomarkers of MSGT, which could allow for more appropriate treatment plans, as well as shedding light on the molecular pathways involved.

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Acknowledgements

We thank Mrs Diana Elizabeth Hearn for her support in revising and translating the manuscript and Ms. Anna Giovinazzo for her great help in organizing the scientific work.

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Author notes

  1. Veronica Seccia and Elena Navari have contributed equally to this work.

Authors and Affiliations

  1. Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, ENT Section, University of Pisa, Pisa, Italy

    Veronica Seccia, Elena Navari, Iacopo Dallan & Augusto Pietro Casani

  2. Department of Pharmacy, University of Pisa, Pisa, Italy

    Elena Donadio, Claudia Boldrini & Maria Rosa Mazzoni

  3. Department of Rheumatology, GIGA Research, Centre Hospitalier Universitaire (CHU) de Liège, University of Liège, Liège, Belgium

    Federica Ciregia

  4. Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Chieti, Italy

    Maurizio Ronci & Antonio Aceto

  5. Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

    Antonio Lucacchini & Laura Giusti

  6. School of Pharmacy, University of Camerino, Via Gentile III da Varano, 62032, Camerino, Italy

    Laura Giusti

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  1. Veronica Seccia
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Correspondence to Laura Giusti.

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This study was approved by the the Local Ethics Committee (Comitato Etico Area Vasta Nord Ovest—CEAVNO, Azienda Ospedaliera Universitaria Pisana; Reference Number 29937). All procedures performed in studies involving human participants were in accordance with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Seccia, V., Navari, E., Donadio, E. et al. Proteomic Investigation of Malignant Major Salivary Gland Tumors. Head and Neck Pathol 14, 362–373 (2020). https://doi.org/10.1007/s12105-019-01040-2

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