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Cross-reactivity of Antibodies Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae With Heat Shock Protein 60 and ATP-Binding Protein Correlates to Reduced Mitochondrial Activity in HIBCPP Choroid Plexus Papilloma Cells

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Abstract

Antibacterial antibodies can cause neurologic side-effects by cross-reactivity with cellular antigens. Here we investigated interactions of antibodies to Neisseria gonorrhoeae (α-NG) - maternal infections by which increases the offspring’s risk for later psychosis—with HIBCPP cells, a cell culture model of choroid plexus epithelium. Immunocytochemistry and Western blotting with α-NG, revealed organelle-like intracellular staining in HIBCPP cells, and labelling of several immunoreactive bands in cellular protein. Two-dimensional Western blotting revealed several immunopositive spots, most prominent of which were identified by mass spectrometry as mitochondrially localized proteins heat shock protein 60 (Hsp60) and ATP-binding protein β-subunit (ATPB). Similarly α-NG interacted with commercial samples of these proteins as revealed by Western blotting. Three alternative methods (JC-1, Janus green and MTT staining) revealed α-NG to cause in HIBCPP cells a significant decrease in mitochondrial activity, which could be reverted by neuroleptic drugs. Immunoreactivity of α-NG with choroid plexus epithelium in human post mortem samples suggests in vivo relevance of these findings. Finally, distinctly different staining patterns of antibodies against Neisseria meningitidis (α-NM), confirmed antibody specificity. To our knowledge this is the first report that α-NG cross-reactivity with Hsp60 and ATPB impairs mitochondrial activity in choroid plexus epithelial cells, pathogenetic relevance of which needs further clarification.

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Acknowledgments

The authors would like to thank Susanne Goldmann for the excellent technical assistance and the University Medicine Göttingen (UMG) for the persistent and reliable support of their work.

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The authors declare no competing financial interests.

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Authors and Affiliations

  1. Institute for Neuroanatomy, University Medicine Göttingen (UMG), Kreuzbergring 36, 37075, Göttingen, Federal Republic of Germany

    B. Reuss

  2. University Children’s Hospital Mannheim, Pediatric Infectious Diseases, Heidelberg University, Heidelberg, Germany

    H. Schroten

  3. The Nippon Dental University, Tokyo, Japan

    H. Ishikawa

  4. Institute for Clinical Chemistry, University Medicine Göttingen (UMG), Göttingen, Germany

    A. R. Asif

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Reuss, B., Schroten, H., Ishikawa, H. et al. Cross-reactivity of Antibodies Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae With Heat Shock Protein 60 and ATP-Binding Protein Correlates to Reduced Mitochondrial Activity in HIBCPP Choroid Plexus Papilloma Cells. J Mol Neurosci 57, 123–138 (2015). https://doi.org/10.1007/s12031-015-0585-7

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