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Mitochondrial DNA Copy Number in Peripheral Blood as a Potential Non-invasive Biomarker for Multiple Sclerosis

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Abstract

The impaired mitochondrial function has been implicated in the pathogenicity of multiple sclerosis (MS), a chronic inflammatory, demyelinating, and neurodegenerative disease of the CNS. Circulating mtDNA copy number in body fluids has been proposed as an indicator for several neurodegenerative diseases, and the altered cerebrospinal fluid mtDNA has been shown as a promising marker for MS. The aim of this study was to determine changes and biomarker potential of circulating mtDNA in peripheral blood in MS. The mtDNA copy number was quantified by real-time PCR in blood samples from 60 patients with relapsing–remitting MS (RRMS) and 64 healthy controls. The RRMS patients had significantly lower circulating mtDNA copy number compared to controls. Subgroup analysis with stratification of RRMS patients based on disease duration under or over 10 years revealed that the mtDNA copy number was significantly lower in the group with longer disease duration. A negative correlation was observed between mtDNA copy number and disease duration. The ROC curve analysis indicated a significant ability of mtDNA copy number to separate RRMS patients from controls with an AUC of 0.859. This is the first study to measure peripheral blood mtDNA copy number in MS patients. Current data suggest that the reduction in peripheral blood mtDNA copy number may be an early event in MS and correlate with the disease progression. The findings of this study indicate that circulating blood-based mtDNA copy number may be a potential non-invasive candidate biomarker for mitochondria-mediated neurodegeneration and MS. This can put forward the clinical applicability over other invasive markers.

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Acknowledgements

The technical support with sample collection and transportation in Saudi Arabia by MAA is gratefully acknowledged. This study was funded by a research Grant (No: 37-PI-01/15) from the College of Medicine and Medical Sciences, Arabian Gulf University, Kingdom of Bahrain.

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Authors and Affiliations

  1. Department of Molecular Medicine and Al-Jawhara Centre for Molecular Medicine, Genetics, and Inherited Disorders, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain

    Ghada Al-Kafaji & Moiz Bakhiet

  2. Al-Jawhara Centre for Molecular Medicine, Genetics, and Inherited Disorders, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain

    Halla F. Bakheit

  3. College of Forensic Sciences, Naif Arab University for Security Sciences, Riyadh, Kingdom of Saudi Arabia

    Maram A. Alharbi

  4. College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain

    Ahmad A. Farahat, Mohamed Jailani & Bashayer H. Ebrahin

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  1. Ghada Al-Kafaji
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  2. Halla F. Bakheit
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  3. Maram A. Alharbi
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  4. Ahmad A. Farahat
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  5. Mohamed Jailani
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  6. Bashayer H. Ebrahin
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  7. Moiz Bakhiet
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Contributions

All authors contributed to the study conception and design. Material preparation was performed by GA-K, HFB, MAA, and AAF. Data collection and analysis were performed by GA-K, MJ, BHE, and MB. The first draft of the manuscript was written by GA-K and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ghada Al-Kafaji.

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Al-Kafaji, G., Bakheit, H.F., Alharbi, M.A. et al. Mitochondrial DNA Copy Number in Peripheral Blood as a Potential Non-invasive Biomarker for Multiple Sclerosis. Neuromol Med 22, 304–313 (2020). https://doi.org/10.1007/s12017-019-08588-w

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