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A Comparative Study of the Preventive Effects of Mitomycin C and Chitosan on Intraarticular Adhesion after Knee Surgery in Rabbits

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Abstract

We sought to compare the preventive effects of mitomycin-C(MMC) and chitosan on intraarticular adhesion after knee surgery in rabbits. For this purpose, 48 New-Zealand rabbits were randomly and equally divided into MMC, chitosan, and control groups. Approximately 10 × 10 mm2 of the cortical bone was removed from both sides of left femoral condyle and the cancellous bone underneath was exposed. The decorticated areas were topically treated with MMC and chitosan while control group was treated with physiological saline. The lower left limb was fixed in flexed position with Kirschner-wire for 4 weeks postoperatively. After 4 weeks, gross and histopathological examination, biochemical analysis, and fibroblast counts were performed on knee intraarticular adhesion in each group. The data show mild membrane-like fibrous intraarticular adhesion, presented in loose, in MMC group. There was moderate intraarticular adhesion in chitosan group while in controls; there was large-size compact fibrous tissue adhesion. Hydroxyproline contents and fibroblast quantity of MMC and chitosan groups were lower (P < 0.05) than that of control group. We, therefore, concluded that MMC and chitosan could prevent intraarticular adhesion of the knee in rabbits by inhibiting fibroblast proliferation and reducing collagenous fiber formation while MMC had a better preventive effect than that of chitosan.

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Acknowledgments

This study was supported by grants from the National Key Program for Developing Basic Research (Grant # 2007CB936104) and the National High Technology Research and Development Program of China (Grant # 2007AA021905). We thank Dr. Xiaojian Cao and Mr. Yu Liu for their excellent technical support.

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Authors and Affiliations

  1. Department of Cadiothoracic, The Second Xiangya Hospital Affiliated with Central-South University, No. 139A, The Middle Renmin Road Changsha, Hunan, 410011, China

    Wang Jingcheng, Yin Bangliang & Lv Guohua

  2. Department of Orthopedics, Subei People’s Hospital, Clinical Medical College of Yangzhou University, Nantong West Road 98, Yangzhou, 225001, China

    Wang Jingcheng, Yan Lianqi, Sun Yu, Wang Daxin, Dai Shanhe, Yu Tangyun, Gu Jiaxiang, Jiang Baichuan, Feng Xinmin, Hu Hansheng & Wang Qiang

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  1. Wang Jingcheng
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  2. Yan Lianqi
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  3. Sun Yu
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Correspondence to Yin Bangliang or Lv Guohua.

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Wang Jingcheng and Yan Lianqi contributed equally to this study as co-first authors.

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Jingcheng, W., Lianqi, Y., Yu, S. et al. A Comparative Study of the Preventive Effects of Mitomycin C and Chitosan on Intraarticular Adhesion after Knee Surgery in Rabbits. Cell Biochem Biophys 62, 101–105 (2012). https://doi.org/10.1007/s12013-011-9266-5

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  • DOI: https://doi.org/10.1007/s12013-011-9266-5

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