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A Combination of a Dairy Product Fermented by Lactobacilli and Galactooligosaccharides Shows Additive Effects on Mineral Balances in Growing Rats with Hypochlorhydria Induced by a Proton Pump Inhibitor

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Abstract

This study aimed to investigate the effects of a combination of a dairy product fermented by lactobacilli (DFL) and galactooligosaccharides (GOS) on mineral balances in growing rats with hypochlorhydria induced by a proton pump inhibitor (PPI). Three-week-old male rats were assigned to receive one of six diets: a control diet, control diets containing 1.6 or 5.0 % GOS, a DFL diet and DFL diets containing 1.6 or 5.0 % GOS for 9 days. From day 5 of the feeding period, half of the rats fed with control diets were subcutaneously administered with saline, whereas the remaining rats were administered with PPI for 5 days. Calcium (Ca), phosphorus (P), magnesium (Mg), iron (Fe) and zinc (Zn) balances were determined from days 6 to 9. PPI administration significantly decreased the apparent absorption of Ca and Fe and increased urinary P excretion, resulting in decreased Ca, Fe and P retention. GOS dose-dependently increased the apparent absorption of Ca, Mg and Fe and urinary Mg excretion and decreased urinary P excretion. DFL significantly increased the apparent absorption of Ca and Mg and urinary Mg excretion. The combination of DFL and GOS additively affected these parameters, resulting in increased Ca, P and Fe retention, and it further increased the apparent absorption and retention of Zn at 5.0 % GOS. In conclusion, the combination of DFL and GOS improves Ca, P and Fe retention in an additive manner and increases the Zn retention in growing rats with hypochlorhydria induced by PPI.

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Acknowledgments

This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.

Conflicts of Interest

We have no conflicts of interest other than that we are employees of Meiji Co., Ltd.

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Authors and Affiliations

  1. Food Science Research Laboratories, Division of Research and Development, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa, 250-0862, Japan

    Satoshi Takasugi, Kinya Ashida, Suyaka Maruyama, Yukari Matsukiyo, Tetsuo Kaneko & Taketo Yamaji

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  1. Satoshi Takasugi
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  2. Kinya Ashida
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  3. Suyaka Maruyama
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  4. Yukari Matsukiyo
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  5. Tetsuo Kaneko
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  6. Taketo Yamaji
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Correspondence to Satoshi Takasugi.

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Takasugi, S., Ashida, K., Maruyama, S. et al. A Combination of a Dairy Product Fermented by Lactobacilli and Galactooligosaccharides Shows Additive Effects on Mineral Balances in Growing Rats with Hypochlorhydria Induced by a Proton Pump Inhibitor. Biol Trace Elem Res 153, 309–318 (2013). https://doi.org/10.1007/s12011-013-9681-0

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  • DOI: https://doi.org/10.1007/s12011-013-9681-0

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