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Hemoglobin Regeneration Efficiency in Anemic Rats: Effects on Bone Mineral Composition and Biomechanical Properties

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Abstract

This study reports the effects of dietary iron (Fe) deficiency and recovery on bone mineral composition and strength in anemic rats submitted to a hemoglobin (Hb) repletion assay. Weanling male Wistar rats were fed a low-Fe diet (12 mg/kg) for 15 days followed by 2 weeks of Fe repletion with diets providing 35 mg Fe/kg as either ferrous sulfate (n = 8) or ferric pyrophosphate (FP; n = 12). At final day of each period (depletion and repletion), Fe-adequate animals were also euthanized. Iron status (blood Hb, Hb Fe pool, Hb regeneration efficiency), tibia mineral concentrations (Ca, Mg, Fe, Cu, and Zn) and biomechanical properties were evaluated. Iron-deficient rats had lower tibia Fe and Mg levels and bone strength when compared to controls. Yield load and resilience were positively related to tibia Mg levels (r = 0.47, P = 0.02 and r = 0.56, P = 0.004, respectively). Iron repletion did not recover tibia Mg concentrations impaired by Fe deficiency. Moreover, bone elastic properties were negatively affected by FP consumption. In conclusion, bone mineral composition and strength were affected by Fe deficiency, whereas dietary Fe source influenced tibia Mg and resistance in the period during which rats were recovering from anemia.

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Abbreviations

FS:

Ferrous sulfate

FP:

Ferric pyrophosphate

HRE:

Hemoglobin regeneration efficiency

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Acknowledgments

The authors thank Dr. José Alfredo Gomes Arêas and Dr. Luiz Antonio Gioielli for their collaboration in the bone biomechanical assays; Ms. Tatiana Garofalo Quintal and Maura Sayuri de Andrade for technical assistance; Fundação de Amparo à Pesquisa do Estado de São Paulo (research project 2006/01735-0) for supporting the research; and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the fellowships awarded to Alexandre R. Lobo. This study was also supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). We also thank Álvaro Augusto Feitosa Pereira for reviewing the manuscript.

Conflicts of Interest

There are no financial, professional, or personal conflicts of interests for any of the authors.

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Authors and Affiliations

  1. Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, Brazil, Av. Prof. Lineu Prestes 580, Bloco 14, 05508 900, São Paulo, São Paulo, Brazil

    Alexandre Rodrigues Lobo, Eduardo Henrique Szpak Gaievski & Célia Colli

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  1. Alexandre Rodrigues Lobo
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  2. Eduardo Henrique Szpak Gaievski
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  3. Célia Colli
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Correspondence to Célia Colli.

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Lobo, A.R., Gaievski, E.H.S. & Colli, C. Hemoglobin Regeneration Efficiency in Anemic Rats: Effects on Bone Mineral Composition and Biomechanical Properties. Biol Trace Elem Res 143, 403–411 (2011). https://doi.org/10.1007/s12011-010-8871-2

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  • DOI: https://doi.org/10.1007/s12011-010-8871-2

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