Abstract
Background
Rodent models are required in studies on the mechanism of Roux-en-Y gastric bypass (RYGB). However, the construction of the model is hard, and there are various causes of death after surgery in rats.
Methods
RYGB models with procedures containing a series of anatomic landmark were established in rats. Optimized procedures during surgery, possible complications after surgery, and corresponding solutions were studied.
Results
With the introduction of perioperative nursing and optimized surgery procedures, less time-consuming surgeries were performed and higher survival rates were achieved. Trouble-shooting data based on death time points are listed and discussed for various causes of failure.
Conclusions
This study provides practical suggestions for investigators to perform RYGB surgery on rats. The troubleshooting suggestions will help operators to efficiently identify problems in their procedures.
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Funding
This study was supported by National Key Basic Research Program of China (No. 2015CB5540007); National Natural Science Foundation of China (Nos. 81472740, 81200276, and 81700488); Natural Science Foundation of Hubei Province of China (No. 2014CFA060 and 2015CFB710); Research Fund of Public Welfare in Health Industry, Health and Family Plan Committee of China (No. 201402015); Natural Science Foundation of Huazhong University of Science and Technology (No. 5001530030); and Health and Family Planning Youth Project Foundation of Hubei Province, China (No. WJ2015Q001).
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The Ethics Committee for Animal Research of Tongji Medicine College approved all procedures in this study.
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Qingbo Wang and Geng Wang are co-first authors.
Guobin Wang and Zefeng Xia are co-corresponding authors
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Fig. S1
a. Validations of body temperature after anesthesia. The rats received 1% sodium pentobarbital (4 ml/kg, i.p.). Rats underwent laparotomy, and the abdominal cavity was kept open for 0.5/1/1.5/2 hour (n = 10 in each group). The abdominal wall was closed, and the rats were returned to cages under SPF room temperature. Anal temperatures of rats were measured afterwards. b. Rats were placed in different room temperatures overnight (25-40 °C, n = 10 in each group) after a 90-100-min RYGB surgery performed by the same surgeon. Survival rates were calculated. c. Timing in different groups (n = 10 in each group): Procedure A, the stomach-jejunum anastomotic group. Procedure B, the jejuno-ileum anastomotic first group. d. Survival curve of rats in different groups (n = 20 in each group): Procedure A, the stomach-jejunum anastomotic group. Procedure B, the jejuno-ileum anastomotic first group. e. Postoperative %Total weight loss(%TWL) in different groups (n = 10 in each group): In RYGB group, body weight decreases rapidly in 1–2 weeks and slowly in 3–4 weeks after surgery, while in Control group, it increases in 4 weeks after surgery. The body weight of Sham group decreases in the first week after surgery, and increases from the second week to the fourth week. At the fourth week, the %TWL approaches the control group (TIF 13868 kb)
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Wang, Q., Wang, G., Hu, C. et al. Optimization of a Technique to Standardize the Rodent Roux-En-Y Gastric Bypass Model and Troubleshooting of Postoperative Failures. OBES SURG 29, 1681–1689 (2019). https://doi.org/10.1007/s11695-019-03789-5
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DOI: https://doi.org/10.1007/s11695-019-03789-5