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Long-Term Evaluation of Biliary Reflux After Experimental One-Anastomosis Gastric Bypass in Rats

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Abstract

Background

Controversy remains regarding biliary reflux after one-anastomosis gastric bypass (OAGB). The aim of this “pilot” study was to analyze biliary reflux and its potential long-term consequences on esogastric mucosae in OAGB-operated rats.

Methods

Diet-induced obese rats were subjected to OAGB (n = 10) or sham (n = 4) surgery and followed up for 16 weeks. Evolution of weight and glucose tolerance was analyzed. Bile acid concentration measurement, histological and qRT-PCR analysis were performed in the esogastric segments.

Results

Weight loss and glucose tolerance were improved after OAGB. Mean bile acid concentration was 4.2 times higher in the esogastric segments of OAGB rats (compared to sham). A foveolar hyperplasia of the gastro-jejunal anastomosis and an eosinophilic polynuclear cell infiltration were observed in OAGB rats. An esophageal hyper-papillomatosis was observed in both groups (OAGB = 50%, sham = 50%). qRT-PCR analysis showed no differences between OAGB and sham mRNA levels of Barrett’s esophagus or esogastric carcinogenic-specific genes. No intestinal metaplasia, dysplasia, or cancer were observed after a 16-week follow-up.

Conclusions

After a 16-week follow-up, this pilot study confirmed the good reproducibility of our OAGB rat model. OAGB rats had not developed any pre-cancerous or cancerous lesions. Further experimental studies with longer term follow-up are required.

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Authors and Affiliations

  1. Inserm UMR 1149, UFR de Médecine Paris Diderot, Université Paris Diderot, Sorbonne Paris Cité, DHU Unity APHP, 75890, Paris, France

    Matthieu Bruzzi, Henri Duboc, Caroline Gronnier, Anne Couvelard, Maude Le Gall & André Bado

  2. Service de Chirurgie Digestive, AP-HP Hôpital Européen Georges Pompidou, 75015, Paris, France

    Matthieu Bruzzi & Jean-Marc Chevallier

  3. ANCRE, EA 4465, Université Paris Descartes, Paris, France

    Matthieu Bruzzi & Jean-Marc Chevallier

  4. Inserm UMR 7203, ERL U1057, Université Paris VI, Paris, France

    Dominique Rainteau

Authors
  1. Matthieu Bruzzi
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  2. Henri Duboc
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  3. Caroline Gronnier
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  4. Dominique Rainteau
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  5. Anne Couvelard
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  6. Maude Le Gall
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  7. André Bado
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  8. Jean-Marc Chevallier
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Corresponding author

Correspondence to Matthieu Bruzzi.

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Ethical Considerations

All applicable institutional and/or national guidelines for the care and use of animals were followed. All experiments were performed in compliance with the European Community guidelines and approved by the Institutional Animal Care and Use Committee (Comité d’Ethique Paris-Nord).

Informed Consent

Informed consent does not apply.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Bruzzi, M., Duboc, H., Gronnier, C. et al. Long-Term Evaluation of Biliary Reflux After Experimental One-Anastomosis Gastric Bypass in Rats. OBES SURG 27, 1119–1122 (2017). https://doi.org/10.1007/s11695-017-2577-x

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  • DOI: https://doi.org/10.1007/s11695-017-2577-x

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