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The Effects of One-Anastomosis Gastric Bypass on Glucose Metabolism in Goto-Kakizaki Rats

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Abstract

Background

The improvement in glucose metabolism after bariatric surgery is well established. The aim of this study was to investigate the hormones and glycemic control in diabetes after a one-anastomosis gastric bypass (OAGB) variant in an animal model of non-obese type 2 diabetes mellitus.

Methods

Thirty-six Goto-Kakizaki rats were randomly assigned to undergo one of the following procedures: OAGB (18 rats) or sham intervention (18 rats). Each group was subdivided into three additional groups according to the time of surgery (early—12 weeks; intermediate—16 weeks; and late—20 weeks). Weight, fasting glycemia, glucose tolerance test (OGTT), and hormone levels (glucagon, insulin, glucagon-like peptide-1 [GLP-1], and glucose-dependent insulinotropic peptide [GIP]) were measured.

Results

All rats maintained their weight. The OGTT showed a significant improvement in glycemic levels in rats with OAGB in all time groups (p < 0.002, for all groups at 60 min). Insulin levels decreased significantly in all animals with OAGB, but glucagon levels increased (glucagon paradoxical response). GLP-1 and GIP increased in rats with OAGB at all times, but was only statistically significant in the early surgery group of GLP-1 (p < 0.005).

Conclusion

OAGB in a non-obese diabetic rat model improves glycemic control, with a significant decrease in glucose and insulin levels. This reduction without weight loss suggests a surgically induced enhancement of pancreatic function. It appears that this improvement occurs, although the GLP-1 levels were significantly increased only in the early stages. The paradoxical response of glucagon should be further evaluated.

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Authors’ contributions

LZ and RA were responsible for the study conception and design, the execution, analyses, and interpretation of the data. LZ and MD have done the drafting of the manuscript. JRT, PE, JAV, and MC generated the experimental data. JAP supervised the handling of animals. LZ, JRT, and MD provided advice on the study concept, conducted specific analyses, and critically revised the manuscript.

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Authors and Affiliations

  1. Universidad Miguel Hernandez de Elche, San Juan de Alicante, Spain

    Lorea Zubiaga, Rafael Abad, Pablo Enriquez & José Antonio Pérez De Gracia

  2. Hospital Universitario de Mostoles, Madrid, Spain

    Jaime Ruiz-Tovar

  3. Hospital Universitario Virgen de Arrixaca, Murcia, Spain

    Juan Antonio Vílchez & Mireia Calzada

  4. International Bariatric Club, Founding Editor Obesity Surgery, Toronto, Canada

    Mervyn Deitel

Authors
  1. Lorea Zubiaga
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  2. Rafael Abad
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  3. Jaime Ruiz-Tovar
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  4. Pablo Enriquez
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  5. Juan Antonio Vílchez
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  6. Mireia Calzada
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  7. José Antonio Pérez De Gracia
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  8. Mervyn Deitel
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Corresponding author

Correspondence to Lorea Zubiaga.

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Funding

This study was funded by Navarro Trípodi Foundation.

Conflict of Interest

The authors declare that they have no competing interests.

Statement of Animal Rights

All applicable institutional, regional, and national guidelines for the care and use of animals were followed, according to experimental ethics committee of the “Miguel Hernández de Elche” University. This article does not contain any studies with human participants performed by any of the authors.

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Zubiaga, L., Abad, R., Ruiz-Tovar, J. et al. The Effects of One-Anastomosis Gastric Bypass on Glucose Metabolism in Goto-Kakizaki Rats. OBES SURG 26, 2622–2628 (2016). https://doi.org/10.1007/s11695-016-2138-8

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  • DOI: https://doi.org/10.1007/s11695-016-2138-8

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