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Implications of Th1 and Th17 cells in pathogenesis of oral lichen planus

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Summary

Oral lichen planus (OLP) is considered a T cell-mediated autoimmune disease with unknown aetiology. T helper cells appear to play an important role in the pathogenesis of OLP. We investigated the possible role of T helper cells, Th1 and Th17, in the lesions and circulation of patients with OLP. Forty patients with OLP and 15 healthy volunteers were recruited. Double immunofluorescence staining was used to detect Th1 and Th17 cells in the OLP lesions, and intracellular cytokine staining and flow cytometry to evaluate the proportion of Th1 and Th17 cells in peripheral blood. The levels of serum interferon (IFN)-γ and interleukin (IL)-17 were assessed by using an enzyme-linked immunosorbent assay. It was found that Th17 cells, as well as Th1 cells, were present in OLP lesions. The proportion of peripheral Th1 and Th17 cells was significantly increased in patients with OLP. The proportion of Th17 cells in atrophic-erosive OLP was elevated as compared with that in reticular OLP. Serum IL-17 levels in OLP patients were significantly higher than in controls, and those in the atrophic-erosive OLP group were increased as compared with the reticular OLP group. However, the levels of serum IFN-γ were slightly decreased in OLP patients. Our data suggested that Th1 and Th17 cells in the local lesions and peripheral blood may be associated with the pathogenesis of OLP, and that IL-17 may be an important proinflammatory cytokine in OLP. These findings enhance our understanding of OLP pathogenesis.

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References

  1. Ingafou M, Leao JC, Porter SR, et al. Oral lichen planus: a retrospective study of 690 British patients. Oral Dis, 2006,12(5):463–468

    Article  PubMed  CAS  Google Scholar 

  2. Sugerman PB, Savage NW, Walsh LJ, et al. The pathogenesis of oral lichen planus. Crit Rev Oral Biol Med, 2002,13(4):350–365

    Article  PubMed  CAS  Google Scholar 

  3. Khan A, Farah CS, Savage NW, et al. Th1 cytokines in oral lichen planus. J Oral Pathol Med, 2003,32(2):77–83

    Article  PubMed  CAS  Google Scholar 

  4. Simark-Mattsson C, Bergenholtz G, Jontell M, et al. Distribution of interleukin-2, -4, -10, tumour necrosis factor-alpha and transforming growth factor-beta mRNAs in oral lichen planus. Arch Oral Biol, 1999,44(6):499–507

    Article  PubMed  CAS  Google Scholar 

  5. Lu R, Zhou G, Du G, et al. Expression of T-bet and GATA-3 in peripheral blood mononuclear cells of patients with oral lichen planus. Arch Oral Biol, 2011,56(5):499–505

    Article  PubMed  CAS  Google Scholar 

  6. Pene J, Chevalier S, Preisser L, et al. Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes. J Immunol, 2008,180(11):7423–7430

    PubMed  CAS  Google Scholar 

  7. Brand S. Crohn’s disease: Th1, Th17 or both? The change of a paradigm: new immunological and genetic insights implicate Th17 cells in the pathogenesis of Crohn’s disease. Gut, 2009,58(8):1152–1167

    Article  PubMed  CAS  Google Scholar 

  8. Korn T, Bettelli E, Oukka M, et al. IL-17 and Th17 Cells. Annu Rev Immunol, 2009,27:485–517

    Article  PubMed  CAS  Google Scholar 

  9. Maruyama T, Kono K, Mizukami Y, et al. Distribution of Th17 cells and FoxP3(+) regulatory T cells in tumor-infiltrating lymphocytes, tumor-draining lymph nodes and peripheral blood lymphocytes in patients with gastric cancer. Cancer Sci, 2010,101(9):1947–1954

    Article  PubMed  CAS  Google Scholar 

  10. Lowes MA, Kikuchi T, Fuentes-Duculan J, et al. Psoriasis vulgaris lesions contain discrete populations of Th1 and Th17 T cells. J Invest Dermatol, 2008,128(5): 1207–1211

    Article  PubMed  CAS  Google Scholar 

  11. Sontheimer RD. Lichenoid tissue reaction/interface dermatitis: clinical and histological perspectives. J Invest Dermatol, 2009,129(5):1088–1099

    Article  PubMed  CAS  Google Scholar 

  12. Zhang L, Yang XQ, Cheng J, et al. Increased Th17 cells are accompanied by FoxP3(+) Treg cell accumulation and correlated with psoriasis disease severity. Clin Immunol, 2010,135(1):108–117

    Article  PubMed  CAS  Google Scholar 

  13. Charazinska-Carewicz K, Ganowicz E, Krol M, et al. Assessment of the peripheral immunocompetent cells in patients with reticular and atrophic-erosive lichen planus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2008,105(2):202–205

    Article  PubMed  Google Scholar 

  14. Liu GX, Sun JT, Yang MX, et al. OPN promotes survival of activated T cells by up-regulating CD44 in patients with oral lichen planus. Clin Immunol, 2011,138(3):291–298

    Article  PubMed  CAS  Google Scholar 

  15. van der Meij EH, van der Waal I. Lack of clinicopathologic correlation in the diagnosis of oral lichen planus based on the presently available diagnostic criteria and suggestions for modifications. J Oral Pathol Med, 2003,32(9):507–512

    Article  PubMed  Google Scholar 

  16. Tao XA, Xia J, Chen XB, et al. FOXP3 T regulatory cells in lesions of oral lichen planus correlated with disease activity. Oral Dis, 2010,16(1):76–82

    Article  PubMed  Google Scholar 

  17. Robertson D, Savage K, Reis-Filho JS, et al. Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue. BMC Cell Biol, 2008,9:13

    Article  PubMed  Google Scholar 

  18. Lodi G, Scully C, Carrozzo M, et al. Current controversies in oral lichen planus: report of an international consensus meeting. Part 1. Viral infections and etiopathogenesis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005,100(1):40–51

    Article  PubMed  Google Scholar 

  19. Szabo SJ, Sullivan BM, Stemmann C, et al. Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells. Science, 2002,295(5553):338–342

    Article  PubMed  CAS  Google Scholar 

  20. Yamamoto T, Yoneda K, Ueta E, et al. Cellular immunosuppression in oral lichen planus. J Oral Pathol Med, 1990,19(10):464–470

    Article  PubMed  CAS  Google Scholar 

  21. Karagouni EE, Dotsika EN, Sklavounou A. Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus. J Oral Pathol Med, 1994,23(1):28–35

    Article  PubMed  CAS  Google Scholar 

  22. Hirota K, Hashimoto M, Yoshitomi H, et al. T cell self-reactivity forms a cytokine milieu for spontaneous development of IL-17+ Th cells that cause autoimmune arthritis. J Exp Med, 2007,204(1):41–47

    Article  PubMed  CAS  Google Scholar 

  23. Tzartos JS, Friese MA, Craner MJ, et al. Interleukin-17 production in central nervous system-infiltrating T cells and glial cells is associated with active disease in multiple sclerosis. Am J Pathol, 2008,172(1):146–155

    Article  PubMed  CAS  Google Scholar 

  24. Elson CO, Cong Y, Weaver CT, et al. Monoclonal anti-interleukin 23 reverses active colitis in a T cell-mediated model in mice. Gastroenterology, 2007,132(7):2359–2370

    Article  PubMed  CAS  Google Scholar 

  25. Luger D, Silver PB, Tang J, et al. Either a Th17 or a Th1 effector response can drive autoimmunity: conditions of disease induction affect dominant effector category. J Exp Med, 2008,205(4):799–810

    Article  PubMed  CAS  Google Scholar 

  26. Stromnes IM, Cerretti LM, Liggitt D, et al. Differential regulation of central nervous system autoimmunity by T(H)1 and T(H)17 cells. Nat Med, 2008,14(3):337–342

    Article  PubMed  CAS  Google Scholar 

  27. O’Connor RA, Prendergast CT, Sabatos CA, et al. Cutting edge: Th1 cells facilitate the entry of Th17 cells to the central nervous system during experimental autoimmune encephalomyelitis. J Immunol, 2008,181(6): 3750–3754

    PubMed  Google Scholar 

  28. Annunziato F, Cosmi L, Santarlasci V, et al. Phenotypic and functional features of human Th17 cells. J Exp Med, 2007,204(8):1849–1861

    Article  PubMed  CAS  Google Scholar 

  29. Yamada H, Nakashima Y, Okazaki K, et al. Th1 but not Th17 cells predominate in the joints of patients with rheumatoid arthritis. Ann Rheum Dis, 2008,67(9):1299–1304

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Stomatology, Huazhong University of Science and Technology, Wuhan, 430030, China

    Sanxiang Xie  (谢三祥), Lei Ding  (丁 蕾) & Shengrong Zhu  (朱声荣)

  2. Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Zhigang Xiong  (熊志刚)

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  1. Sanxiang Xie  (谢三祥)
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  2. Lei Ding  (丁 蕾)
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  4. Shengrong Zhu  (朱声荣)
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Correspondence to Shengrong Zhu  (朱声荣).

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Xie, S., Ding, L., Xiong, Z. et al. Implications of Th1 and Th17 cells in pathogenesis of oral lichen planus. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 451–457 (2012). https://doi.org/10.1007/s11596-012-0078-7

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  • DOI: https://doi.org/10.1007/s11596-012-0078-7

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