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Expression of transcription factor Oct4 in bladder cancer cell line T24 and its effects on the biological characteristics of the cells

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Summary

The expression of octamer binding factor 4 (Oct4) gene in bladder cancer cell line T24 and its effects on the biological characteristics of the cells were investigated. RT-PCR and Western blot were employed to detect the expression of Oct4 in T24 cells. The changes of biological characteristics in T24 cells were analyzed before and after gene-silencing by Boyden chamber and MTT. The results showed that the expression of Oct4 gene was detectable in T24 cells by RT-PCR and Western blot. The expression of Oct4 gene and protein was down-regulated by siRNA, and average number of transwell cells in interference group, negative control group and blank control group was 101.40±4.56, 104.20±10.03 and 111.00±11.90, respectively. There was significant difference in the proliferation ability of the cells from 48 h, 72 h to 96 h after the interference by siRNA between interference group and negative group or blank control group (P<0.05). It was suggested that Oct4 gene was related with proliferation ability of T24 cells, but not with invasive capability.

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Authors and Affiliations

  1. Department of Emergency Center and Pathology, The First Hospital of Zhengzhou University, Institute of Clinical Medical Research of Henan Universities, Zhengzhou, 450052, China

    Zhiqiang Zhu  (朱志强), Jianguo Wen  (文建国), Xiangyu Zheng  (郑湘予), Daoxie Wang  (王道协), Qingwei Wang  (王庆伟) & Changhui Fan  (樊长辉)

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  1. Zhiqiang Zhu  (朱志强)
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  2. Jianguo Wen  (文建国)
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  3. Xiangyu Zheng  (郑湘予)
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  4. Daoxie Wang  (王道协)
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  5. Qingwei Wang  (王庆伟)
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  6. Changhui Fan  (樊长辉)
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Correspondence to Jianguo Wen  (文建国).

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Zhu, Z., Wen, J., Zheng, X. et al. Expression of transcription factor Oct4 in bladder cancer cell line T24 and its effects on the biological characteristics of the cells. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 73–76 (2009). https://doi.org/10.1007/s11596-009-0115-3

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  • DOI: https://doi.org/10.1007/s11596-009-0115-3

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