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Southern rice black-streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae

  • Articles/Plant Pathology
  • Published:
Chinese Science Bulletin

Abstract

For the past several years, a novel dwarf disease has been observed on rice (Oryza sativa) in some regions of Guangdong Province and Hainan Province, southern China. Infected plants showed stunting, dark leaf and small enations on stem and leaf back. Typical Fijivirus viroplasma containing crystalline arrayed spherical virons approximately 70–75 nm in diameter and tubular structures were detected in ultrathin sections by an electron microscope in parenchyma phloem cells of the infected plants. The virus was transmitted to rice seedlings by white-backed planthoppers, Sogatella furcifera (Hemiptera: Delphacidae), collected in the diseased fields. Analysis of dsRNA extracts from infected plants revealed ten linear segments, which were similar to the electrophoretic profile of Rice black-streaked dwarf virus (RBSDV). RT-PCR with a single primer which matched to a linker sequence ligated to both 3′ ends of the viral genomic dsRNAs resulted in amplification of genome segments 9 (S9) and 10 (S10) cDNA products. The complete nucleotide sequences of S9 and S10 were obtained from clones of the RT-PCR amplicon exhibited characteristic properties of Fijivirus including low GC content (34.5% and 35.6%), genus conserved 5′ and 3′ termini sequences and similar genome organization. Blast searches indicated that the sequences of S9 and S10 shared 68.8%–74.9% and 67.1%–77.4% nucleotide identities with those of viruses in the Fijivirus group 2, respectively. These values were similar to those among other viruses in the Fijivirus group 2 and considerably lower than those among RBSDV isolates. Phylogenetic trees based on S9 and S10 nucleotide sequences and their putative amino acid sequences showed that this virus represented a separate branch among other Fijiviruses. The virus was also detected by a nested RT-PCR assay in corn (Zea mays), barnyard grass (Echinochloa crusgalli), Juncellus serotinus and flaccidgrass (Pennisetum flaccidum) in and/or adjacent to the infected rice fields. It is proposed that this virus be considered as a new species, Southern rice black-streaked dwarf virus, in the group 2 of the genus Fijivirus in the family Reoviridae.

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References

  1. Milne R G, de Vas M, Harding R M, et al. Genus Fijivirus. In: Fauquet C M, Mayo M A, Maniloff J, et al, eds. Virus Taxomony: Classification and nomenclature of viruses. Eighth Report of the International Committee on the Taxonomy of Viruses. San Diego: Elsevier Academic Press, 2005. 534–542

    Google Scholar 

  2. Chen S X, Zhang Q Y. Advance in researches on rice black-streaked dwarf disease and maize rough dwarf disease in China (in Chinese). Acta Phytophyl Sin, 2005, 32: 97–103

    Google Scholar 

  3. Zhang H M, Lei J L, Chen J P, et al. A dwarf disease on rice, wheat and maize from Zhejiang and Hebei is caused by Rice black-streaked dwarf virus (in Chinese). Virol Sin, 2001, 16(3): 246–251

    Google Scholar 

  4. Büchen-Osmond C. The Universal Virus Database, ICTVdB, version 4. New York: Columbia University, 2006 (http://www.ncbi.nlm.nih.gov/ICTVdb)

    Google Scholar 

  5. Zhou G H, Xu D L, Li H P. Molecular identification of the pathogen causing rice dwarf disease in Guangdong. Proceedings of the 2004 Annual Meeting of Chinese Society of Plant Pathology (in Chinese). Beijing: China Agricuture Press, 2004. 210–212

    Google Scholar 

  6. Dodds J A, Morris T J, Jordan R L. Plant viral double-stranded RNA. Annu Rev Phytopathol, 1984, 22: 151–168

    Article  CAS  Google Scholar 

  7. Potgieter A C, Steele A D, van Dijk A A. Cloning of complete genome sets of six dsRNA viruses using an improved cloning method for large dsRNA genes. J Gen Virol, 2002, 83: 2215–2223

    PubMed  CAS  Google Scholar 

  8. Matthijnssens J, Rahman M, Martella V, et al. Full genomic analysis of human rotavirus strain B4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission. J Virol, 2006, 80: 3801–3810

    Article  PubMed  CAS  Google Scholar 

  9. Chen S X, Hong J, Lu Y P, et al. Study on the relation between RBSDV infcting stages in maize vein cell and active transmission of Laodelphax striatellus Fallen (in Chinese). Virol Sin, 2005, 19(2): 153–157

    Google Scholar 

  10. Zhang H, Chen J, Adams M. Molecular characterization of segments 1 to 6 of Rice black-streaked dwarf virus from China provides the complete genome. Arch Virol, 2001, 146: 2331–2339

    Article  PubMed  CAS  Google Scholar 

  11. Wang Z, Fang S, Xu J, et al. Sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. Virus Genes, 2003, 27: 163–168

    Article  PubMed  Google Scholar 

  12. Nakashima N, Noda H. Nonpathogenic Nilaparvata lugens reovirus is transmitted to the brown planthopper through rice plant. Virology, 1995, 207: 303–307

    Article  PubMed  CAS  Google Scholar 

  13. Distéfano A J, Conci L R, Munoz Hidalgo M, et al. Sequence analysis of genome segments S4 and S8 of Mal de Río Cuarto virus (MRCV): Evidence that the virus should be a separate Fijivirus species. Arch Virol, 2002, 147: 1699–1709

    Article  PubMed  Google Scholar 

  14. Noda H, Nakashima N, Omura T. Cloning of the Nilaparvata lugens reovirus genome: Conserved terminal nucleotide sequences and nucleotide sequence of genome segment S10. J Gen Virol, 1994, 75: 221–225

    Article  PubMed  CAS  Google Scholar 

  15. Nakashima N, Koizumi M, Watanabe H, et al. Complete nucleotide sequence of the Nilaparvata lugens reovirus: A putative member of the genus Fijivirus. J Gen Virol, 1996, 77: 139–146

    Article  PubMed  CAS  Google Scholar 

  16. Isogai M, Uyeda I, Lindsten K. Taxonomic characteristics of Fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome. J Gen Virol, 1998, 79: 1479–1485

    PubMed  CAS  Google Scholar 

  17. Distéfano A J, Conci L R, Munoz Hidalgo M, et al. Sequence and phylogenetic analysis of genome segments S1, S2, S3 and S6 of Mal de Río Cuarto virus, a newly accepted Fijivirus species. Virus Res, 2003, 92: 113–121

    Article  PubMed  Google Scholar 

  18. Guzman F A, Distéfano A J, Arneodo J D, et al. Sequencing of the bicistronic genome segments S7 and S9 of Mal de Río Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus. Arch Virol, 2007, 152: 565–573

    Article  PubMed  CAS  Google Scholar 

  19. Marzach C, Boccardo G, Nuss D. Cloning of the maize rough dwarf virus genome: Molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains within a single genomic segment. Virology, 1991, 180: 518–526

    Article  Google Scholar 

  20. Marzach C, Antoniazzi S, de Aquilio M, et al. The double-stranded RNA genome of maize rough dwarf Fijivirus contains both mono and dicistronic segments. Eur J Plant Pathol, 1996, 102: 601–605

    Article  Google Scholar 

  21. Distéfano A J, Hopp H E, del Vas M. Sequence analysis of genome segments S5 and S10 of Mal de Río Cuarto virus (Fijivirus, Reoviridae). Arch Virol, 2005, 150: 1241–1248

    Article  PubMed  Google Scholar 

  22. Giménez-Pecci M P, Conci L R, Truol G, et al. Molecular diversity of ecologically distinct Mal de Río Cuarto virus isolates based on restriction fragment length polymorphism (RFLPs) and genome sequence analysis of segments 1, 7, 9 and 10. Arch Virol, 2007, 152: 1341–1351 (doi: 10.1007/s00705-007-0944-y)

    Article  PubMed  Google Scholar 

  23. Jiang J, Ridley A W, Tang H, et al. Genetic variability of genome segments 3 and 9 of Fiji disease virus field isolates. Arch Virol, 2008, 153: 838–848 (doi: 10.1007/s00705-008-0058-1)

    Article  Google Scholar 

  24. Nault L R. Transmission biology, vector specificity, and evolution of planthopper transmitted plant viruses. In: Denno R F, ed. Planthoppers. New York: Chapman and Hall, 1994. 429–448

    Google Scholar 

  25. Power A G. Insect transmission of plant viruses: A constraint on virus variability. Curr Opin Plant Biol, 2000, 3: 336–340

    Article  PubMed  CAS  Google Scholar 

  26. Milne R G, Boccardo G, Dal Bo E, et al. Association of maize rough dwarf virus with Mal de Río Cuarto in Argentina. Phytopathology, 1983, 739(12): 1290–1292

    Article  Google Scholar 

  27. Shen J H, Shang J M, Liu G J. Management of the white-backed planthopper, Sogatella furcifera in China: A mini-review (in Chinese). Chin J Rice Sci, 2003, 17(S): 7–22

    Google Scholar 

  28. Bao Y X, Xu X Y, Wang J Q, et al. Analysis of the atmospheric dynamical backgrounds for the great immigration events of white backed planthopper (Sogatella furcifera) (in Chinese). Acta Ecol Sin, 2007, 27(11): 4527–4535

    Google Scholar 

  29. Wang Y K, Zhai B P. Re-migration capacity of the white-backed planthopper, Sogatella furcifera (in Chinese). Acta Entomol Sin, 2004, 47(4): 467–473

    Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratory of Plant Virology, South China Agricultural University, Guangzhou, 510642, China

    GuoHui Zhou, JingJung Wen, DongLin Xu & ShuGuang Zhang

  2. Plant Protection Station, Agricultural Department of Hainan Province, Haikou, 570003, China

    DeJiang Cai & Peng Li

Authors
  1. GuoHui Zhou
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  2. JingJung Wen
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  3. DeJiang Cai
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  4. Peng Li
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  5. DongLin Xu
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  6. ShuGuang Zhang
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Correspondence to GuoHui Zhou.

Additional information

Supported by the Guangdong Provincial Department of Science and Technology (Grant No. 2003B21604), Natural Science Foundation of Guandong Province (Grant No. 5006669) and Agricultural Department of Hainan Province

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Zhou, G., Wen, J., Cai, D. et al. Southern rice black-streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae . Chin. Sci. Bull. 53, 3677–3685 (2008). https://doi.org/10.1007/s11434-008-0467-2

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  • DOI: https://doi.org/10.1007/s11434-008-0467-2

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