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Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus

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Abstract

A quantitative 1H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin was calculated from the ratio of the intensity of the amygdalin H-2 signal at δ 6.50 ppm in pyridine-d 5 to that of the hexamethyldisilane (HMD) signal at 0 ppm. The HMD concentration was corrected by the International System of Units (SI) traceability with certified reference material (CRM)-grade bisphenol A. qHNMR revealed the amygdalin contents to be 2.72 and 3.13 % in 2 lots of Persicae semen, 3.62 and 5.19 % in 2 lots of Armeniacae semen, and 0.23 % in Mume fructus. Thus, we demonstrated the utility of this method for the quantitative analysis of crude drugs.

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Acknowledgments

We thank Dr. N Sugimoto of the National Institute of Health Sciences for his kind advice on the measurements by qHNMR.

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Authors and Affiliations

  1. Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan

    Rie Tanaka, Akane Nitta & Akito Nagatsu

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  1. Rie Tanaka
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  2. Akane Nitta
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  3. Akito Nagatsu
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Correspondence to Akito Nagatsu.

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Tanaka, R., Nitta, A. & Nagatsu, A. Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus. J Nat Med 68, 225–230 (2014). https://doi.org/10.1007/s11418-013-0783-y

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  • DOI: https://doi.org/10.1007/s11418-013-0783-y

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