Abstract
Although arsenic is an infamous carcinogen, it has been effectively used to treat acute promyelocytic leukemia, and can induce cell cycle arrest or apoptosis in human solid tumors. Previously, we had demonstrated that opposing effects of ERK1/2 and JNK on p21 expression in response to arsenic trioxide (As2O3) are mediated through the Sp1 responsive elements of the p21 promoter in A431 cells. Presently, we demonstrate that Sp1, and c-Jun functionally cooperate to activate p21 promoter expression through Sp1 binding sites (−84/−64) by using DNA affinity binding, chromatin immunoprecipitation, and promoter assays. Surprisingly, As2O3-induced c-Jun(Ser63/73) phosphorylation can recruit TGIF/HDAC1 to the Sp1 binding sites and then suppress p21 promoter activation. We suggest that, after As2O3 treatment, the N-terminal domain of c-Jun phosphorylation by JNK recruits TGIF/HDAC1 to the Sp1 sites and then represses p21 expression. That is, TGIF is involved in As2O3-inhibited p21 expression, and then blocks the cell cycle arrest.
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Abernathy CO, Liu YP, Longfellow D, Aposhian HV, Beck B, Fowler B, Goyer R, Menzer R, Rossman T, Thompson C, Waalkes M (1999) Arsenic: health effects, mechanisms of actions, and research issues. Environ Health Perspect 107(7):593–597
Murgo AJ (2001) Clinical trials of arsenic trioxide in hematologic and solid tumors: overview of the National Cancer Institute Cooperative Research and Development Studies. Oncologist 6: 22–28
Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ (1993) The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75(4):805–816
Zhou BP, Liao Y, Xia W, Spohn B, Lee MH, Hung MC (2001) Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells. Nat Cell Biol 3(3):245–252
Biggs JR, Kudlow JE, Kraft AS (1996) The role of the transcription factor Sp1 in regulating the expression of the WAF1/CIP1 gene in U937 leukemic cells. J Biol Chem 271(2):901–906
Ohtsubo M, Gamou S, Shimizu N (1998) Antisense oligonucleotide of WAF1 gene prevents EGF-induced cell-cycle arrest in A431 cells. Oncogene 16(6):797–802
Huang HS, Liu ZM, Ding L, Chang WC, Hsu PY, Wang SH, Chi CC, Chuang CH (2006) Opposite effect of ERK1/2 and JNK on p53-independent p21WAF1/CIP1 activation involved in the arsenic trioxide-induced human epidermoid carcinoma A431 cellular cytotoxicity. J Biomed Sci 13(1):113–125
Liu ZM, Huang HS (2006) As2O3-induced c-Src/EGFR/ERK signaling is via Sp1 binding sites to stimulate p21WAF1/CIP1 expression in human epidermoid carcinoma A431 cells. Cell Signal 18(2):244–255
Derijard B, Hibi M, Wu IH, Barrett T, Su B, Deng T, Karin M, Davis RJ (1994) JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76(6):1025–1037
Pessah M, Prunier C, Marais J, Ferrand N, Mazars A, Lallemand F, Gauthier JM, Atfi A (2001) c-Jun interacts with the corepressor TG-interacting factor (TGIF) to suppress Smad2 transcriptional activity. Proc Natl Acad Sci U S A 98(11):6198–6203
Gripp KW, Wotton D, Edwards MC, Roessler E, Ades L, Meinecke P, Richieri-Costa A, Zackai EH, Massague J, Muenke M, Elledge SJ (2000) Mutations in TGIF cause holoprosencephaly and link NODAL signalling to human neural axis determination. Nat Genet 25(2):205–208
Melhuish TA, Gallo CM, Wotton D (2001) TGIF2 interacts with histone deacetylase 1 and represses transcription. J Biol Chem 276(34):32109–32114
Sharma M, Sun Z (2001) 5′TG3′ interacting factor interacts with Sin3A and represses AR-mediated transcription. Mol Endocrinol 15(11):1918–1928
Wotton D, Knoepfler PS, Laherty CD, Eisenman RN, Massague J (2001) The Smad transcriptional corepressor TGIF recruits mSin3. Cell Growth Differ 12(9):457–463
Datto MB, Yu Y, Wang XF (1995) Functional analysis of the transforming growth factor beta responsive elements in the WAF1/Cip1/p21 promoter. J Biol Chem 270(48):28623–28628
Huang HS, Chen CJ, Chang WC (1999) The CCAAT-box binding factor NF-Y is required for the expression of phospholipid hydroperoxide glutathione peroxidase in human epidermoid carcinoma A431 cells. FEBS Lett 455(1–2):111–116
Boulon S, Dantonel JC, Binet V, Vie A, Blanchard JM, Hipskind RA, Philips A (2002) Oct-1 potentiates CREB-driven cyclin D1 promoter activation via a phospho-CREB- and CREB binding protein-independent mechanism. Mol Cell Biol 22(22):7769–7779
Huang HS, Chang WC, Chen CJ (2002) Involvement of reactive oxygen species in arsenite-induced downregulation of phospholipid hydroperoxide glutathione peroxidase in human epidermoid carcinoma A431 cells. Free Radic Biol Med 33(6):864–873
Kardassis D, Papakosta P, Pardali K, Moustakas A (1999) c-Jun transactivates the promoter of the human p21(WAF1/Cip1) gene by acting as a superactivator of the ubiquitous transcription factor Sp1. J Biol Chem 274(41):29572–29581
Chen BK, Chang WC (2000) Functional interaction between c-Jun and promoter factor Sp1 in epidermal growth factor-induced gene expression of human 12(S)-lipoxygenase. Proc Natl Acad Sci U S A 97(19):10406–10411
Taunton J, Hassig CA, Schreiber SL (1996) A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 272(5260):408–411
Sowa Y, Orita T, Minamikawa S, Nakano K, Mizuno T, Nomura H, Sakai T (1997) Histone deacetylase inhibitor activates the WAF1/Cip1 gene promoter through the Sp1 sites. Biochem Biophys Res Commun 241(1):142–150
Banks EB, Crish JF, Welter JF, Eckert RL (1998) Characterization of human involucrin promoter distal regulatory region transcriptional activator elements-a role for Sp1 and AP1 binding sites. Biochem J 331:61–68
Lin MC, Almus-Jacobs F, Chen HH, Parry GC, Mackman N, Shyy JY, Chien S (1997) Shear stress induction of the tissue factor gene. J Clin Invest 99(4):737–744
Karin M, Liu Z, Zandi E (1997) AP-1 function and regulation. Curr Opin Cell Biol 9(2):240–246
Cruzalegui FH, Hardingham GE, Bading H (1999) c-Jun functions as a calcium-regulated transcriptional activator in the absence of JNK/SAPK1 activation. EMBO J 18(5):1335–1344
Grimm C, Wenzel A, Behrens A, Hafezi F, Wagner EF, Reme CE (2001) AP-1 mediated retinal photoreceptor apoptosis is independent of N-terminal phosphorylation of c-Jun. Cell Death Differ 8(8):859–867
Struhl K (1998) Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 12(5):599–606
Behre G, Whitmarsh AJ, Coghlan MP, Hoang T, Carpenter CL, Zhang DE, Davis RJ, Tenen DG (1999) c-Jun is a JNK-independent coactivator of the PU.1 transcription factor. J Biol Chem 274(8):4939–4946
Faggioli L, Costanzo C, Donadelli M, Palmieri M (2004) Activation of the Interleukin-6 promoter by a dominant negative mutant of c-Jun. Biochim Biophys Acta 1692(1):17–24
Zagariya A, Mungre S, Lovis R, Birrer M, Ness S, Thimmapaya B, Pope R (1998) Tumor necrosis factor alpha gene regulation: enhancement of C/EBPbeta-induced activation by c-Jun. Mol Cell Biol 18(5):2815–2824
Chen LC, Chen BK, Chang WC (2005) Activating protein 1-mediated cyclooxygenase-2 expression is independent of N-terminal phosphorylation of c-Jun. Mol Pharmacol 67(6):2057–2069
Soucy NV, Ihnat MA, Kamat CD, Hess L, Post MJ, Klei LR, Clark C, Barchowsky A (2003) Arsenic stimulates angiogenesis and tumorigenesis in vivo. Toxicol Sci 76(2):271–279
Straub AC, Stolz DB, Vin H, Ross MA, Soucy NV, Klei LR, Barchowsky A (2007) Low level arsenic promotes progressive inflammatory angiogenesis and liver blood vessel remodeling in mice. Toxicol Appl Pharmacol 222(3):327–336
Liu B, Pan S, Dong X, Qiao H, Jiang H, Krissansen GW, Sun X (2006) Opposing effects of arsenic trioxide on hepatocellular carcinomas in mice. Cancer Sci 97(7):675–681
Gartel AL, Radhakrishnan SK (2005) Lost in transcription: p21 repression, mechanisms, and consequences. Cancer Res 65(10):3980–3985
Acknowledgements
We are greatly indebted to Dr. Wen-Chang Chang for his valuable discussions. This work was supported by grant NSC95-2320-B-006-055-MY3 from National Science Council of Taiwan.
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Liu, ZM., Huang, HS. Inhibitory role of TGIF in the As2O3-regulated p21WAF1/CIP1 expression. J Biomed Sci 15, 333–342 (2008). https://doi.org/10.1007/s11373-007-9232-9
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DOI: https://doi.org/10.1007/s11373-007-9232-9