Abstract
Dengue virus (DENV) is an arthropod-borne human pathogen that represents a severe public health threat in both endemic and non-endemic regions. So far, there is no licensed vaccine or specific drugs available for dengue fever. A fifteen-amino-acid-long peptide that includes the NGR motif was chemically synthesized and conjugated with keyhole limpet hemocyanin. A standard immunization protocol was followed for the production of polyclonal antibodies by immunizing rabbits against the synthetic peptide. The immune response elicited high-titer polyclonal antibodies with the reactivity of the anti-peptide antibody against both synthetic peptide and four serotypes of DENV confirmed by DOT-ELISA. Neutralizing activity of anti-peptide antibody was found to be cross-reactive and effective resulting in 60% reduction of infectivity at 1:200 dilution in all four serotypes of DENV. Our findings have the potential to further improve our understanding of virus–host interactions and provide new insights into neutralizing antibodies and could also be used as a drug target.
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Acknowledgments
The work was supported by Bioinformatics Infrastructure Facility (BT/BI/25/017/2012), Bioinformatics Division, Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi. Authors would like to thank Dr.R. Paramasivan, Scientist E, CRME (ICMR), Madurai, for the comments and suggestions.
Author contributions
JAM conceived or designed study. AJ, PL, and RS coordinated and analyzed data, and wrote the paper. SC and JAM performed research, analyzed data, and wrote the paper.
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The study protocol was approved by the Institutional Animal Ethical Committee (IAEC) of the Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai. The rabbits were handed over to pet lovers after the completion of the work.
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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.
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Mary, J.A., Jittmittraphap, A., Chattanadee, S. et al. A synthetic peptide derived from domain III envelope glycoprotein of Dengue virus induces neutralizing antibody. Virus Genes 54, 25–32 (2018). https://doi.org/10.1007/s11262-017-1508-1
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DOI: https://doi.org/10.1007/s11262-017-1508-1