Abstract
The virus most often reported in papaya (Carica papaya L.) is papaya ringspot (PRSV). The aim of this work was the molecular genomic characterization of a Mexican severe isolate of PRSV-P “Mex-VrPO” (isolate from the State of Veracruz in Paso de Ovejas) as well as its comparison with other isolates from other world regions. The linear, assembled, single-strand positive sense RNA genome of PRSV-P Mex-VrPO was 10320 nt in length (excluding the poly(A) tail) and contained a single large predicted ORF with 3344 aa. The comparative analysis of our PRSV isolates and five others reported before, showed the most variable proteins were P1, P3, 6 K and CP with 13–33%, 5–7%, 6–9% and 5–9% divergence respectively. The most conserved ones were CI, NIb and HC-Pro (2–3%, 3–5% and 4–5%). The phylogenetic analysis showed a close relation between the Mexican (Mex-VrPO) and Hawaiian (PRSV-P HA) isolates. This work provided the first opportunity to establish the foundation for (1) understanding whole genome and polyprotein variability between Asian and American PRSV isolates, and (2) elucidating major trends in the relative evolution of viral cistrons as deduced from in silico recombination analyses.
Similar content being viewed by others
References
D.E. Purcifull, J. Edwarson, E. Hiebert, Papaya ringspot virus. Descriptions of plant viruses. Commonwealth Mycological Institute and Association of Applied Biologists, United Kingdom (1984)
D.D. Shukla, C.W. Ward, A.A. Brunt, The Potyviridae (CAB International, Wallinford, UK, 1994)
D. Teliz, G. Mora, D. Nieto, D. Gonsalves, E. Garcia, L. Matheis, C. Avila, Revista Mexicana de Fitopatología 9, 64 (1991)
M.F. Bateson, R.E. Lines, P. Revill, W. Chaleeprom, C.V. Ha, A.J. Gibbs, J.L. Dale, J. Gen. Virol. 83, 2575 (2002)
R.K. Jain, J. Sharma, A.S. Sivakumar, P.K. Sharma, A.S. Byadgi, A.K. Verma, A. Varma, Arch. Virol. 149, 2435 (2004)
H.K.C. Laidlaw, D.M. Persley, C.K. Pallaghy, I.D. Godwin, Arch. Virol. 149, 1633 (2004)
L. Silva-Rosales, N. Becerra-Leor, S. Ruiz-Castro, D. Teliz-Ortiz, J.C. Noa-Carrazana, Arch. Virol. 145, 835 (2000)
J.C. Noa-Carrazana, D. González-de-León, B.S. Ruiz-Castro, D. Piñero, L. Silva-Rosales, Plant Dis. 90, 1004 (2006)
S.-D. Yeh, F.J. Jan, C.H. Chiang, T.J. Doong, M.C. Chen, P.H. Chung, H.J. Bau, J. Gen. Virol. 73, 2531 (1992)
C.-H. Wang, (2002)
C.-H. Wang, S.-D. Yeh, R.J. Chiu, D. Gonsalves, Plant Dis. 71, 491 (1987)
P. Attasart, G. Charoensilp, S. Kertbundit, S. Panyim, M. Juricek, Acta Virol. 46, 241 (2002)
M. Worobey, E.C. Holmes, J. Gen. Virol. 80, 2535 (1999)
R.H. Converse, R.R. Martin, ELISA methods for plant viruses. American Phytopathological Society, St. Paul, MN, 1990
J.A. Lindbo, W.G. Dougherty, Virology 189, 725 (1992)
S. Ruiz-Castro, L. Silva-Rosales, Revista Mexicana de Fitopatología 15, 83 (1997)
F. Sanger, S. Nicklen, A.R. Coulson, Proc. Natl. Acad. Sci. USA 74, 5463 (1977)
J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673 (1994)
M.O. Salminen, J.K. Carr, D.S. Burke, F.E. McCutchan, AIDS Res. Hum. Retroviruses 11, 1423 (1995)
M.J. Gibbs, J.S. Armstrong, A.J. Gibbs, Bioinformatics 16, 573 (2000)
J. Maynard Smith, J. Mol. Evol. 34, 126 (1992)
D. Posada, K.A. Crandall, Proc. Natl. Acad. Sci. USA 98, 13757 (2001)
M. Padidam, S. Sawyer, C.M. Fauquet, Virology 265, 218 (1999)
D. Martin, E. Rybicki, Bioinformatics 16, 562 (2000)
G. McGuire F. Wright, Bioinformatics 16, 130 (2000)
C.-H. Wang, S.-D. Yeh, Arch. Virol. 142, 271 (1997)
D.R. Gallie, Ann. Rev. Plant Physiol. Plant Mol. Biol. 44, 77 (1993)
A. Chyi-Ying, A.-B. Shyu, Trends Biochem. Sci. 20, 465 (1995)
M.E. Aleman-Verdaguer, C. Goudou-Urbino, J. Dubern, R.N. Beachy, C. Fauquet, J. Gen. Virol. 78, 1253 (1997)
D. Posada, Mol. Biol. Evol. 19, 708 (2002)
C. Desbiez, H. Lecoq, Arch. Virol. 149, 1619 (2004)
R.C. Larsen, P.N. Miklas, K.L. Druffel, Phytopathology 95, 1037 (2003)
D. Guo, M.-L. Rajama, M. Saarma, J.P. Valkonen, J. Gen. Virol. 82, 935 (2001)
K. Mukherjeel, Y. Verma, S.K. Chakrabarti, S.M.P. Khurana, Virus Genes 29, 229 (2004)
J.C. Carrington, J. Verchot, J. Virol. 69, 3668 (1995)
Acknowledgments
This research was funded by the project SEMARNAT 2002 CO1-304/A-1. JC Noa-Carrazana acknowledges financial support from Cinvestav and CONACyT (scholarship no.111976).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Noa-Carrazana, J.C., González-de-León, D. & Silva-Rosales, L. Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates. Virus Genes 35, 109–117 (2007). https://doi.org/10.1007/s11262-006-0039-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-006-0039-y