Abstract
The gene encoding the large (L) protein and the genome termini of the dolphin strain of cetacean morbillivirus (CeMV) were sequenced. The CeMV genome is 15702 nucleotides long and has been compared with other available morbillivirus genome sequences in regards to the “rule of six” and the “phase” of any particular nucleotide, defined as its position within a given hexamer, which here is defined as a group of six nucleotides starting from the 3′ end of the genomic RNA. With exception of the position of the start of the F gene, the phase of the transcription start sites of each gene is strictly conserved between the morbilliviruses, but each gene is in a different phase. The lengths of gene transcripts differ between viruses by multiples of six nucleotides with exception of the M and F transcripts. The differences between the various morbilliviruses result from deletions or insertions of multiples of six nucleotides in the 3′ and 5′ UTRs of the different viral genes. The four bases were distributed non-randomly over the six positions in the hexamer boxes. However, the distribution patterns of each of the four bases indicated that multiples of three were more prevalent than those of six nucleotides. This reflected the positions of nucleotides in codons and codon usage in the reading frames. The L protein of CeMV was found to be 2183 amino acids in length and similar to that of MV and RPV. The CeMV L protein sequence was found to be equidistant beween those of the CDV/PDV and MV/RPV subgroups of the morbilliviruses. This concurs with the analyses carried out on the other structural proteins.
Similar content being viewed by others
References
M.J.G. Appel E.P.J. Gibbs S.J. Martin V. ter Meulen B.K. Rima J.R. Stephenson W.P. Taylor (1981) NoChapterTitle E. Kurstag C. Kurstak (Eds) Comparative Diagnosis of Viral Diseases Academic Press New York 235–300
S.L. Cosby S. McQuaid N. Duffy C. Lyons B.K. Rima G.M. Allan S.J. McCullough S. Kennedy J.A. Smyth F. McNeilly C. Craig C. Õrvell (1988) Nature 336 115–116 Occurrence Handle10.1038/336115b0
B.W.J. Mahy T. Barrett S. Evans E.C. Anderson C.J. Bostock (1988) Nature 336 115 Occurrence Handle10.1038/336115a0
S. Kennedy J.A. Smyth P.F. Cush S.J. McCullough G.M. Allan S. McQuaid (1988) Nature 336 21 Occurrence Handle1:STN:280:DyaL1M%2FktVWrtA%3D%3D Occurrence Handle3185717 Occurrence Handle10.1038/336021a0
M. Domingo L. Ferrer M. Pumarola A. Marco J. Plana S. Kennedy M. McAliskey B.K. Rima (1990) Nature 348 21 Occurrence Handle1:STN:280:DyaK3M%2FktlSgug%3D%3D Occurrence Handle2234055 Occurrence Handle10.1038/348021a0
G. Bolt M. Blixenkrone-Møller E. Gottschalck R.G.A. Wishaupt M.J. Welsh J.A.P. Earle B.K. Rima (1994) Vir Res 34 291–304 Occurrence Handle1:CAS:528:DyaK2MXitFOqu78%3D Occurrence Handle10.1016/0168-1702(94)90129-5
D.E. Griffin W.J. Bellini (1996) NoChapterTitle B.N. Fields (Eds) Virology Lippincott-Raven Publishers New York 1267–1312
W.J. Bellini G. Englund S. Rozenblatt H. Arnheiter C.D. Richardson (1985) J Virol 53 908–919 Occurrence Handle1:CAS:528:DyaL2MXhsVeqt7Y%3D Occurrence Handle3882996 Occurrence Handle254726
R. Cattaneo K Kaelin K. Baczko M.A. Billeter (1989) Cell 56 759–764 Occurrence Handle1:CAS:528:DyaL1MXhvVKqsLY%3D Occurrence Handle2924348 Occurrence Handle10.1016/0092-8674(89)90679-X
F. Radecke M.A. Billeter (1995) Cur Top Microbiol Immunol 191 181–192 Occurrence Handle1:CAS:528:DyaK2MXnsVentb4%3D
M.D. Baron Y. Kamata V. Barras L. Goatley T. Barrett (1996) J. Gen Virol 77 3041–3046 Occurrence Handle1:CAS:528:DyaK2sXhvVKrsw%3D%3D Occurrence Handle9000095 Occurrence Handle10.1099/0022-1317-77-12-3041
M.A. McIlhatton M.D. Curran B.K. Rima (1997) J Gen Virol 78 571–578 Occurrence Handle1:CAS:528:DyaK2sXhsVWrt7Y%3D Occurrence Handle9049407 Occurrence Handle10.1099/0022-1317-78-3-571
M.D. Curran D.K. Clarke B.K. Rima (1991) J Gen Virol 72 443–447 Occurrence Handle1:CAS:528:DyaK3MXltVOhsLc%3D Occurrence Handle1993883 Occurrence Handle10.1099/0022-1317-72-2-443
M.D. Curran D. O’Loan S. Kennedy B.K. Rima (1992) J Gen Virol 73 1189–1194 Occurrence Handle1:CAS:528:DyaK3sXit1KrtLw%3D Occurrence Handle1588321 Occurrence Handle10.1099/0022-1317-73-5-1189
M. Blixenkrone-Møller G. Bolt E. Gottschalck M. Kenter (1994) J Gen Virol 75 2829–2834 Occurrence Handle7931174 Occurrence Handle10.1099/0022-1317-75-10-2829
M. Blixenkrone-Møller G. Bolt T.D. Jensen T. Harder V. Svansson (1996) Vir Res 40 47–55 Occurrence Handle10.1016/0168-1702(95)01254-0
G. Bolt S. Alexandersen M. Blixenkrone-Møller (1995) J Gen Virol 76 3051–3058 Occurrence Handle1:CAS:528:DyaK2MXhtVSjtb3J Occurrence Handle8847510 Occurrence Handle10.1099/0022-1317-76-12-3051
C. Tapparel D. Maurice L. Roux (1998) J Virol 72 3117–3128 Occurrence Handle1:CAS:528:DyaK1cXhvVOjtLY%3D Occurrence Handle9525637 Occurrence Handle109762
P. Calain L. Roux (1993) J Virol 67 4822–4830 Occurrence Handle1:CAS:528:DyaK3sXlsFegsLw%3D Occurrence Handle8392616 Occurrence Handle237869
D. Kolakofsky T. Pelet D. Garcin S. Hausmann J. Curran L. Roux (1998) J Virol 72 891–899 Occurrence Handle1:CAS:528:DyaK1cXlt1CmsA%3D%3D Occurrence Handle9444980 Occurrence Handle124558
B.K. Rima N.V. McFerran (1997) J Gen Virol 78 2859–2870 Occurrence Handle1:CAS:528:DyaK2sXntVyhsLc%3D Occurrence Handle9367373 Occurrence Handle10.1099/0022-1317-78-11-2859
B.K. Rima J.A.P. Earle K. Baczko V. ter Meulen U.G. Liebert S.C. Carstens J. Carabaña M. Caballero M.L. Celma R. Fernandez-Muñoz (1997) J Gen Virol 78 97–106 Occurrence Handle1:CAS:528:DyaK2sXmslKgug%3D%3D Occurrence Handle9010291 Occurrence Handle10.1099/0022-1317-78-1-97
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rima, B.K., Collin, A.M.J. & Earle, J.A.P. Completion of the Sequence of a Cetacean Morbillivirus and Comparative Analysis of the Complete Genome Sequences of Four Morbilliviruses. Virus Genes 30, 113–119 (2005). https://doi.org/10.1007/s11262-004-4588-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-004-4588-7