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Occurrence and antimicrobial resistance of Salmonella serovars in apparently healthy slaughtered sheep and goats of central Ethiopia

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Abstract

The present study was undertaken to determine the occurrence, distribution and antimicrobial resistance pattern of Salmonella serovars in apparently healthy slaughtered sheep and goats in central Ethiopia. A total 1224 samples consisting of faeces, mesenteric lymph nodes, liver, spleen, and abdominal and diaphragmatic muscle samples were collected from 104 sheep and 100 goats. Salmonella was isolated from 12 of 104 (11.5%) sheep and 3 of 100 (3%) goats. Of the total 624 and 600 samples examined from sheep and goats, 18 (2.9%) and 4 (0.7%), respectively, were Salmonella positive. The 22 Salmonella isolates belonged to 9 different serovars. The common serovars isolated were S. typhimurium, followed by S. heidelberg, S. reading, S. give, and S. poona. Seven of the 22 isolates (31.8%) were multidrug-resistant to various antimicrobials.

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Abbreviations

ABM:

abdominal muscle

Amc:

amoxicillin/clavulanic acid

Amp:

ampicillin

Cef:

cephalothin

Chl:

chloramphenicol

DIM:

diaphragmatic muscle

ICEPT:

International Centre for Enteric Phage Typing

ISO:

International Organization for Standardization

MLN:

mesenteric lymph node

NCCLS:

National Committee for Clinical Laboratory Standards

NLEP:

National Laboratory for Enteric Pathogens

Spt:

spectinomycin

Str:

streptomycin

Sul:

sulfisoxazole

Sxt:

sulfamethoxazole/trimethoprim

Tet:

tetracycline

Tmp:

trimethoprim

References

  • Abdel-Ghani, M., Mohomed, A.H. and Yassein, S., 1987. Occurrence of Salmonella in sheep and goats in Egypt. Journal of the Egyptian Veterinary Medical Association, 47, 161–170

    Google Scholar 

  • Alemayehu, D., Molla, B. and Muckle, A., 2003. Prevalence and antimicrobial resistance pattern of Salmonella isolates from apparently healthy slaughtered cattle in Ethiopia. Tropical Animal Health and Production, 35, 309–319

    Article  PubMed  CAS  Google Scholar 

  • Anderson, E.S., 1964. The phage typing of Salmonella other than S. typhi. In: E. Van Oye (ed.), The World Problem of Salmonellosis, (Dr. W. Junk Publishers, The Hague), 89–100

    Google Scholar 

  • Anderson, E.S. and Williams, R.E.O., 1956. Bacteriophage typing of enteric pathogens and staphylococci and its use in epidemiology. Journal of Clinical Pathology, 9, 94–114

    PubMed  CAS  Google Scholar 

  • Anderson, E.S., Ward, L.R., De Saxe, M.J. and De Sa, J.D.H., 1977. Bacteriophage typing designations of Salmonella typhimurium. Journal of Hygiene, 78, 297–300

    PubMed  CAS  Google Scholar 

  • Callow, B.R., 1959. A new phage typing scheme for Salmonella typhimurium. Journal of Hygiene, 57, 346–359

    Article  PubMed  CAS  Google Scholar 

  • D'Aoust, J.Y., 1994. Salmonella and the international food trade. International Journal of Food Microbiology, 24, 11–31

    Article  PubMed  Google Scholar 

  • D'Aoust, J.Y., Sewell, A.M., Dally, E. and Greco, P., 1992. Antibiotic resistance of agricultural and foodborne Salmonella isolates in Canada: 1986–1989. Journal of Food Protection, 55, 428–434

    Google Scholar 

  • Demczuk, W., Soule, G., Clark, C., Ackermann, H.-W., Easy, R., Kahkhria, R., Rodgers, F. and Ahmed, R., 2003. Phage-based typing scheme for Salmonella enterica serovar heidelberg, a causative agent of food poisonings in Canada. Journal of Clinical Microbiology, 41, 4279–4284

    Article  PubMed  CAS  Google Scholar 

  • Ewing, W.H., 1986. Serological identification of Salmonella. In: W.H. Ewing (ed.), Edwards and Ewing's Identification of Enterobacteriaceae, 4th edn, (Elsevier Science, New York), 201–238

    Google Scholar 

  • Gebreyes, W.A. and Altier, C., 2002. Molecular characterization of multidrug-resistant Salmonella enterica subsp. enterica serovar typhimurium isolates from swine. Journal of Clinical Microbiology, 40, 2813–2822

    Article  PubMed  CAS  Google Scholar 

  • Gebre-Yohannes, A., Mamo, K. and Wolde, H., 1987. R-factor mediated multi-drug resistance in Salmonella typhimurium isolates. Ethiopian Medical Journal, 25, 53–54

    PubMed  CAS  Google Scholar 

  • International Organization for Standardization (ISO), 1998. ISO 6579: Microbiology of Food and Animal Feeding Stuff—Horizontal Method for the Detection of Salmonella, (ISO, Geneva)

    Google Scholar 

  • Kumar, S., Saxena, S.P. and Gupta, B.K., 1973. Carrier rate of salmonellas in sheep and goats and its public health significance. Journal of Hygiene (Cambridge), 71, 43–47

    CAS  Google Scholar 

  • Larkin, C., Poppe, C., McNa, B., McEwen, B., Mahdi, A. and Odumeru, J., 2004. Antibiotic resistance of Salmonella isolated from hog, beef and chicken carcass samples from provincially inspected abattoirs in Ontario. Journal of Food Protection, 67, 448–455

    PubMed  CAS  Google Scholar 

  • Leegaard, T.M., Van Gestel, M.H., Petit, P.L.C. and Van De Klundert, J.A.M., 1996. Antibiotic resistance mechanisms in Salmonella species causing bacteraemia in Malawi and Kenya. APMIS, 104, 302–306

    PubMed  CAS  Google Scholar 

  • Mache, A., Mengistu, Y. and Cowley, S., 1997. Salmonella serogroups identified from adult diarrhoeal outpatients in Addis Ababa, Ethiopia: antibiotic resistance and plasmid profile analysis. East African Medical Journal, 74, 183–186

    PubMed  CAS  Google Scholar 

  • Molla, B., Mesfin, A. and Alemayehu, D., 2003. Multiple antimicrobial-resistant Salmonella serovars isolated from chicken carcass and giblets in Debre Zeit and Addis Ababa, Ethiopia. Ethiopian Journal of Health Development, 17, 131–139

    Google Scholar 

  • Molla, B., Mohammed, A. and Salah, W., 2004. Salmonella prevalence and distribution of serotypes in apparently healthy slaughtered camels (Camelus dromedarius) in Eastern Ethiopia. Tropical Animal Health and Production, 36(5), 451–458

    Article  PubMed  CAS  Google Scholar 

  • Nabbut, N.H. and Al-Nakhli, H.M., 1982. Incidence of Salmonella in lymph nodes, spleen and faeces of sheep and goats slaughtered in the Riyadh public abattoir. Journal of Food Protection, 45, 1314–1317

    Google Scholar 

  • National Committee for Clinical Laboratory Standards (NCCLS), 1999. Performance standards for antimicrobial disc and dilution susceptibility tests for bacteria isolated from animals and humans, (Approved standard. NCCLS document M31-A; NCCLS, Villanova, PA)

    Google Scholar 

  • Popoff, M.Y. and Le Minor, L., 1997. Antigenic Formulas of the Salmonella Serovars, 7th edn, (WHO Collaborating Centre for Research on Salmonella, Institut Pasteur, Paris)

  • Quinn, P.J., Carter, M.E., Markey, B.K. and Carter, C.R., 1994. Veterinary Clinical Microbiology, (Wolfe Publishing, London)

    Google Scholar 

  • Samuel, J.L., Eccles, J.A. and Francis, J., 1981. Salmonella in the intestinal tract and associated lymph nodes of sheep and cattle. Journal of Hygiene, 87, 225–232

    Article  PubMed  CAS  Google Scholar 

  • Sharma, R.N., Musonda, M.M., Munang, H.M., Muyoyeta, P. and Sinyangwe, P.G., 1996. Salmonella isolation from animals in the Republic of Zambia. Bulletin of Animal Health and Production in Africa, 44, 5–7

    Google Scholar 

  • Shipp, C.R. and Rowe, B., 1980. A mechanised microtechnique for Salmonella serotyping. Journal of Clinical Pathology, 33, 595–597

    PubMed  CAS  Google Scholar 

  • Sierra, M.L., Gonzalez-Fandos, E., Garcia-Lopez, M.L., Fernandez, M.C.G. and Prieto, M., 1995. Prevalence of Salmonella, Yersinia, Aeromonas, Campylobacter, and cold-growing Escherichia coli on freshly dressed lamb carcasses. Journal of Food Protection, 58, 1183–1185

    Google Scholar 

  • Smeltzer, T., Thomas, R. and Collins, G., 1980. The role of equipment having accidental or indirect contact with the carcase in the spread of Salmonella in an abattoir. Australian Veterinary Journal, 56, 14–17

    PubMed  CAS  Google Scholar 

  • Ward, L.R., de Sa, J.D.H. and Rowe, B., 1987. A phage-typing scheme for Salmonella enteritidis. Epidemiology and Infection, 99, 291–294

    Article  PubMed  CAS  Google Scholar 

  • Woldemariam, E., Molla, B., Alemayehu, D. and Muckle, A., 2005. Prevalence and distribution of Salmonella in apparently healthy slaughtered sheep and goats in Debre Zeit, Ethiopia. Small Ruminant Research, 58, 19–24

    Article  Google Scholar 

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, Gondar University, Gondar, Ethiopia

    W. Molla

  2. Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Addis Ababa University, Debre Zeit, Ethiopia

    B. Molla & D. Alemayehu

  3. Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA

    D. Alemayehu

  4. OIÉ Reference Laboratory for Salmonellosis, Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada

    A. Muckle, L. Cole & E. Wilkie

Authors
  1. W. Molla
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  2. B. Molla
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  3. D. Alemayehu
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  4. A. Muckle
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  5. L. Cole
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  6. E. Wilkie
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Correspondence to B. Molla.

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Molla, W., Molla, B., Alemayehu, D. et al. Occurrence and antimicrobial resistance of Salmonella serovars in apparently healthy slaughtered sheep and goats of central Ethiopia. Trop Anim Health Prod 38, 455–462 (2006). https://doi.org/10.1007/s11250-006-4325-4

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