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Forsythiaside A Exhibits Anti-inflammatory Effects in LPS-Stimulated BV2 Microglia Cells Through Activation of Nrf2/HO-1 Signaling Pathway

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Abstract

Inflammation and oxidative stress have been reported to play critical roles in the pathogenesis of neurodegenerative disease. Forsythiaside A, a phenylethanoside product isolated from air-dried fruits of Forsythia suspensa, has been reported to have anti-inflammatory and antioxidant effects. In this study, the anti-inflammatory effects of forsythiaside A on LPS-stimulated BV2 microglia cells and primary microglia cells were investigated. The production of inflammatory mediators TNF-α, IL-1β, NO and PGE2 were detected in this study. NF-κB, nuclear factor-erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1) expression were detected by western blot analysis. Our results showed that forsythiaside A significantly inhibited LPS-induced inflammatory mediators TNF-α, IL-1β, NO and PGE2 production. LPS-induced NF-κB activation was suppressed by forsythiaside A. Furthermore, forsythiaside A was found to up-regulate the expression of Nrf2 and HO-1. In conclusion, this study demonstrates that forsythiaside A inhibits LPS-induced inflammatory responses in BV2 microglia cells and primary microglia cells through inhibition of NF-κB activation and activation of Nrf2/HO-1 signaling pathway.

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Acknowledgments

This study was supported by Grants from the ‘Neuroprotective effects of microRNA-124 on dopaminergic neurons in Parkinson’s disease and its related mechanism (81371397)’ and The Guangdong Provincial Clinical Medical Centre for Neurosurgery (No. 2013B0204000 05).

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Authors and Affiliations

  1. The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China

    Yue Wang & Shizhong Zhang

  2. Department of Neurosurgery, No. 208 Hospital of PLA, Changchun, China

    Yue Wang

  3. Department of Encephalopathy, The Affiliated Hospital of Changchun Chinese Medicine University, Changchun, China

    Hongfei Zhao

  4. The Third Affiliated Hospital of Southern Medical University, Guangzhou, China

    Chuangxin Lin

  5. Nursing Department, No. 461 Hospital of PLA, Changchun, China

    Jie Ren

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  1. Yue Wang
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  2. Hongfei Zhao
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  3. Chuangxin Lin
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Correspondence to Shizhong Zhang.

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Wang, Y., Zhao, H., Lin, C. et al. Forsythiaside A Exhibits Anti-inflammatory Effects in LPS-Stimulated BV2 Microglia Cells Through Activation of Nrf2/HO-1 Signaling Pathway. Neurochem Res 41, 659–665 (2016). https://doi.org/10.1007/s11064-015-1731-x

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  • DOI: https://doi.org/10.1007/s11064-015-1731-x

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