Abstract
Hippocampal slices have been widely used to investigate electrophysiological and metabolic neuronal parameters, as well as parameters of astroglial activity including protein phosphorylation and glutamate uptake. S100B is an astroglial-derived protein, which extracellularly plays a neurotrophic activity during development and excitotoxic insult. Herein, we characterized S100B secretion in acute hippocampal slices exposed to different concentrations of K+ and Ca2+ in the extracellular medium. Absence of Ca2+ and/or low K+ (0.2 mM KCl) caused an increase in S100B secretion, possibly by mobilization of internal stores of Ca2+. In contrast, high K+ (30 mM KCl) or calcium channel blockers caused a decrease in S100B secretion. This study suggests that exposure of acute hippocampal slices to low- and high-K+ could be used as an assay to evaluate astrocyte activity by S100B secretion: positively regulated by low K+ (possibly involving mobilization of internal stores of Ca2+) and negatively regulated by high-K+ (likely secondary to influx of K+).
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Acknowledgments
This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), FINEP/Rede IBN 01.06.0842-00 and INCT-National Institute of Science and Technology for Excitotoxicity and Neuroprotection. We would like to thank Ms. Liz Marina Bueno dos Passos for technical support with NSE measurement.
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Nardin, P., Tortorelli, L., Quincozes-Santos, A. et al. S100B Secretion in Acute Brain Slices: Modulation by Extracellular Levels of Ca2+ and K+ . Neurochem Res 34, 1603–1611 (2009). https://doi.org/10.1007/s11064-009-9949-0
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DOI: https://doi.org/10.1007/s11064-009-9949-0