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Comparison of the Efficiencies of Three Neural Induction Protocols in Human Adipose Stromal Cells

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Abstract

The aim of this study was to compare the neural differentiation potential and the expression of neurotrophic factors (NTFs) in differentiated adipose-derived stem cells (ADSCs) using three established induction protocols, serum free (Protocol 1), chemical reagents (Protocol 2), and spontaneous (Protocol 3) protocols. Protocol 1 produced the highest percentage of mature neural-like cells (MAP2ab+). Protocol 2 showed the highest percentage of immature neural-like cells (β-tubulin III+), but the neural-like state was transient and reversible. Protocol 3 caused ADSCs to differentiate spontaneously into immature neural-like cells, but not into mature neural cell types. The neural-like cells produced by Protocol 1 lived the longest in culture with little cell death, but Protocol 2 and 3 led to the significant cell death. Therefore, Protocol 1 is the most efficient among these protocols. Additionally, soon after differentiation, the mRNA levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in dADSCs were sharply decreased by Protocol 1 and 2 (acute induction protocol), but not by Protocol 3 (chronic induction protocol). The results indicate that NTFs played an important role in neural differentiation via acute responses to NGF and BDNF, but not chronically during the transdifferentiation process.

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Acknowledgments

This work was supported by grants from the Natural Science Foundation of China (No. U0632008), Foundation for Key Sci-Tech Research Projects of Guangdong (No. 2008A030201019, 2007-05/06-7005206) and Guangzhou (No. 09B52120112, 2008A1-E4011-6), Foundation for Medical and Scientific Technology Research of Guangdong (No. A2009293).

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Authors and Affiliations

  1. Neurosurgery Institute of Guangdong, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253# Gongye Road, 510282, Guangzhou, China

    Dong-Xiang Qian, Hong-Tian Zhang, Xu Ma, Xiao-Dan Jiang & Ru-Xiang Xu

  2. Department of Neurosurgery, The Third Affiliated Hospital, Guangzhou Medical College, 510150, Guangzhou, China

    Dong-Xiang Qian

  3. Department of Neurosurgery, The General Hospital of Beijing PLA, 100700, Beijing, China

    Hong-Tian Zhang & Ru-Xiang Xu

  4. Department of Neurosurgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, 510120, Guangzhou, China

    Xu Ma

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  1. Dong-Xiang Qian
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  2. Hong-Tian Zhang
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Correspondence to Xiao-Dan Jiang or Ru-Xiang Xu.

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Hong-Tian Zhang contributed equally with Dong-Xiang Qian.

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Qian, DX., Zhang, HT., Ma, X. et al. Comparison of the Efficiencies of Three Neural Induction Protocols in Human Adipose Stromal Cells. Neurochem Res 35, 572–579 (2010). https://doi.org/10.1007/s11064-009-0101-y

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  • DOI: https://doi.org/10.1007/s11064-009-0101-y

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