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Analysis of HGF, MACC1, C-met and apoptosis-related genes in cervical carcinoma mice

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Abstract

To understand the underlying pharmacological basis and the molecular mechanism of Taxol in therapy of cervical carcinoma (CC) disease, we need to explore the effect of Taxol on CC-related genes and pro-apoptosis and anti-apoptosis genes expression. Immunohistochemistry, western blot and reverse transcription-polymerase chain reaction were applied to examine postive expression levels of Bcl-2, Bax and Caspase-3, HGF, MACC1, Caspase-3 and C-met proteins and MACC1 mRNA expression in tumour of CC mice. Results showed that treatment of Taxol could increase the inhibition rate of tumour growth, positive expression levels of Caspase-3, Bax and decrease positive expression levels of Bcl-2 and Bcl-2/Bax, expression levels of HGF, MACC1 and C-met proteins and MACC1 mRNA in tumour tissue of CC mice. It can be concluded that inhibitory activity of Taxol against tumour growth in CC mice is closely associated with its modulating positive expression of Bcl-2, Bax, Caspase-3, expression of HGF, MACC1, Caspase-3 and C-met proteins and MACC1 mRNA in tumour of CC mice. In conclusion, HGF, MACC1 and C-met genes involve into malignant cervical tumors occurrence, development and prognosis, and might become potential molecular target therapy site of cervical cancer. Taxol intervention may serve as a multi-targeted CC therapeutic capable of inducing selective cancer cell death.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Yancheng City First People’s Hospital, Yancheng, 215006, China

    Xiao-Ping Chen & Xin-Ping Ren

  2. Department of Obstetrics and Gynecology, First Affiliated Hospital of Soochow University, Suzhou, 215006, China

    You-Guo Chen & Zong-Ji Shen

  3. Department of Pathology, Yancheng city First People’s Hospital, Yancheng, 215006, China

    Jian-Yun Lan

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  1. Xiao-Ping Chen
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  2. Xin-Ping Ren
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  4. You-Guo Chen
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Correspondence to You-Guo Chen or Zong-Ji Shen.

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Xiao-Ping Chen and Xin-Ping Ren have equally contributed to this work.

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Chen, XP., Ren, XP., Lan, JY. et al. Analysis of HGF, MACC1, C-met and apoptosis-related genes in cervical carcinoma mice. Mol Biol Rep 41, 1247–1256 (2014). https://doi.org/10.1007/s11033-013-2969-5

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  • DOI: https://doi.org/10.1007/s11033-013-2969-5

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