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Subclinical inflammation/oxidation as revealed by altered gene expression profiles in subjects with impaired glucose tolerance and Type 2 diabetes patients

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Abstract

Although subclinical inflammation and oxidative stress are implicated in the aetiology of diabetes, there are hardly any studies in prediabetes. Therefore, we made an attempt to study the gene expression pattern of certain inflammatory/oxidative genes using lymphocytes from Type 2 diabetic patients, impaired glucose tolerance (IGT), and normal glucose tolerance (NGT) subjects. Compared to NGT group, interleukin-6, tumor necrosis factor-α (TNF-α), p22Phox NADPH oxidase, and thioredoxin interacting protein (TXNIP) mRNA levels were higher and suppressor of cytokine signaling (SOCS-3) mRNA was lower in subjects with IGT and diabetes. The mean (±SE) levels of thiobarbituric acid reactive substances and protein carbonyl content were also elevated in glucose intolerant subjects. In multiple linear regression analysis, TXNIP and TNF-α showed a significant association with HbA1c even after adjusting for TBARS and PCO (TXNIP: β = 1.70, P < 0.01; TNF-α: β = 1.86, P < 0.01). Increased subclinical inflammation/oxidation is seen in Asian Indians with not only Type 2 diabetes but also IGT.

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Acknowledgements

This work was supported by research grants from the Department of Science and Technology (DST-FIST), Indian Council of Medical Research (ICMR) and the Department of Biotechnology (DBT), Government of India, New Delhi, India. KG and FM acknowledges the financial assistance (Senior Research Fellowship) from the Council of Scientific & Industrial Research (CSIR), New Delhi, India. This is paper no. 49 from the Chennai Urban Rural Epidemiology Study (CURES).

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Madras Diabetes Research Foundation &, Dr. Mohan’s Diabetes Specialities Centre, 4, Conran Smith Road, Gopalapuram, Chennai, 600 086, India

    Kuppan Gokulakrishnan, Kutuva Tulasi Mohanavalli, Finny Monickaraj & Muthuswamy Balasubramanyam

  2. Department of Diabetology, Madras Diabetes Research Foundation &, Dr. Mohan’s Diabetes Specialities Centre, 4, Conran Smith Road, Gopalapuram, Chennai, 600 086, India

    Viswanathan Mohan

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  1. Kuppan Gokulakrishnan
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  2. Kutuva Tulasi Mohanavalli
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  3. Finny Monickaraj
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  5. Muthuswamy Balasubramanyam
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Correspondence to Muthuswamy Balasubramanyam.

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Gokulakrishnan, K., Mohanavalli, K.T., Monickaraj, F. et al. Subclinical inflammation/oxidation as revealed by altered gene expression profiles in subjects with impaired glucose tolerance and Type 2 diabetes patients. Mol Cell Biochem 324, 173–181 (2009). https://doi.org/10.1007/s11010-008-9996-x

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