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Predictive value of salivary microRNA-320a, vascular endothelial growth factor receptor 2, CRP and IL-6 in Oral lichen planus progression

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Abstract

Introduction

MicroRNA (miRNA) 320a and vascular endothelial growth factor receptor 2 (VEGFR-2) expression as the angiogenic biomarkers might be therapeutic targets in Oral lichen planus (OLP). IL-6 and C-reactive protein (CRP) could be prognostic in OLP, dysplastic OLP and Oral squamous cell carcinoma (OSCC). Therefore, their salivary detections as the noninvasive tools were aimed in this study.

Materials and methods

Histopathologic examinations were carried out to distinguish the patients with dysplastic OLP and OSCC. Salivary microRNA expression analysis was performed using RT-qPCR. IL-6 and CRP levels were also measured in saliva via ELISA method. VEGFR-2 expression in various sections was evaluated using immunohistochemistry.

Results

A significant decrease in salivary microRNA-320a in dysplastic OLP and OSCC but not in OLP without dysplasia was found. VEGFR-2 visualization confirmed the increasing angiogenic process in these cases. A significant increase in IL-6 level was detected in cases with OLP, dysplastic OLP and OSCC. CRP levels also showed a significant increase in dysplastic OLP and OSCC. A positive correlation between IL-6 and CRP levels was found.

Conclusion

Identification of the salivary microRNA-320a and hs-CRP might provide a convenient noninvasive predictive tool for dysplastic OLP, whereas IL-6 could be a diagnostic and therapeutic target in both OLP without dysplasia and dysplastic OLP cases.

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Acknowledgments

We would like to thank the Iran University of Medical Science for supporting this project.

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Authors and Affiliations

  1. Hematology and Blood Banking Department, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Minoo Shahidi & Mohammad Saeed Gholami

  2. Department of Oral Medicine, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Soudeh Jafari & Masoumeh Mahdipour

  3. Department of Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mahmood Barati

  4. Cellular and Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran

    Minoo Shahidi

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  1. Minoo Shahidi
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  2. Soudeh Jafari
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  3. Mahmood Barati
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  4. Masoumeh Mahdipour
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  5. Mohammad Saeed Gholami
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Correspondence to Minoo Shahidi.

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There is no conflict of interests that could be perceived as influencing the objective of this research.

Ethical considerations

The work was approved by the Medical Ethics Committee of Iran University of Medical Sciences (IR.IUMS.REC.1395.25316) and written informed consent was obtained from all cases.

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Shahidi, M., Jafari, S., Barati, M. et al. Predictive value of salivary microRNA-320a, vascular endothelial growth factor receptor 2, CRP and IL-6 in Oral lichen planus progression. Inflammopharmacol 25, 577–583 (2017). https://doi.org/10.1007/s10787-017-0352-1

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  • DOI: https://doi.org/10.1007/s10787-017-0352-1

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