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Breast Implant Texturization Does Not Affect the Crosstalk Between MSC and ALCL Cells

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Abstract

In the last decade, there has been a growing interest about the possible association between anaplastic large cell lymphoma (ALCL) and breast implants (BIA-ALCL). Many variables, such as breast implants texturization, have been investigated. Breast implants often lead to the formation of a periprosthetic capsule, characterized by inflammation. The presence of the inflamed capsule has been found in the majority of patients with BIA-ALCL. Inflammation may be sustained or counteracted by mesenchymal stem cells (MSCs) by the secretion of pro- or anti-inflammatory cytokines. MSCs were isolated from three capsules surrounding micro-textured (micro-MSCs) and from three capsules surrounding macro-textured (macro-MSCs) implants; after characterization, MSCs were co-cultured with KI-JK cells (a cell line derived from the cutaneous form of ALCL). The secretion of cytokines related to inflammation, the proliferation rate, and the expression of genes referred to pro-tumoral mechanisms were evaluated. Co-cultures of KI-JK cells with micro- or macro-MSCs gave the same results about the secretion of cytokines (increase of IL10, G-CSF, and TGF-β1 and decrease of IL4, IL5, IL12, IL13, IL17A, IFN-γ (p < 0.05) with respect to mock sample), expression of selected genes (increase for ACVR1, VEGF, TGF-βR2, CXCL12, and MKi67 (p < 0.05) with respect to control sample), and the proliferation rate (no variation between mock and co-cultured samples). Our results suggest that MSCs derived from capsules surrounding micro- and macro-textured implants display the same effects on the ALCL cells.

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Author notes

  1. Monia Orciani and Miriam Caffarini contributed equally to this work.

Authors and Affiliations

  1. Department of Clinical and Molecular Sciences- Histology, Università Politecnica delle Marche, Via Tronto 10/A, 60126, Ancona, Italy

    Monia Orciani, Miriam Caffarini & Roberto Di Primio

  2. Department of Experimental and Clinical Medicine – Clinic of Plastic and Reconstructive Surgery, Università Politecnica delle Marche, Ancona, Italy

    Matteo Torresetti & Giovanni Di Benedetto

  3. Department of Clinical and Molecular Sciences- Clinic of Dermatology, Università Politecnica delle Marche, Ancona, Italy

    Anna Campanati

  4. Clinic of Plastic and Reconstructive Surgery of Udine, University of Udine, Udine, Italy

    Piercamillo Parodi

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  1. Monia Orciani
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Correspondence to Monia Orciani.

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This study was approved by the Università Politecnica delle Marche Ethical Commitee and was conducted in accordance with the Declaration of Helsinki.

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Electronic supplementary material

Supplementary Fig. 1

Flow cytometry results of micro-MSCs cells (a) and macro-MSCs (b). Representative FACScan analyses of cell-surface antigen expression, as indicated. Solid gray histograms refer to the negative control (IgG1 isotype control-FITC labeled). No significative differences were noted among the six cultures of MSCs. (PNG 912 kb)

High resolution image (TIF 228 kb)

Supplementary Fig. 2

Mesenchymal differentiation from micro-MSCs (a-b-c-d) and macro-MSCs (e-f-g-h). Representative images of osteogenic differentiation assessment by ALP staining (a, e) and identification of CaPO4 crystals by von Kossa reaction (b, f); adipocyte differentiation confirmed by Oil red staining (c, g); chondrogenic differentiation demonstrated by positive acid mucopolisaccarid coloration by Safranin-O (d, h). No differences were noted among micro- and macro-MSCs. Scale bar 50um (PNG 3615 kb)

High resolution image (TIF 879 kb)

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Orciani, M., Caffarini, M., Torresetti, M. et al. Breast Implant Texturization Does Not Affect the Crosstalk Between MSC and ALCL Cells. Inflammation 42, 721–730 (2019). https://doi.org/10.1007/s10753-018-0930-4

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