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LncRNA ZEB1-AS1 contributes to STAT3 activation by associating with IL-11 in B-lymphoblastic leukemia

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Abstract

Objective

To investigate the role of lncRNA ZEB1-AS1 in B-lineage acute lymphoblastic leukemia (B-ALL).

Results

ZEB1-AS1 levels were aberrantly up-regulated in B-ALL. All correlated with STAT3 activation and IL-11 production. Moreover, a high level of ZEB1-AS1 predicted poor prognosis of B-ALL patients. Mechanistically, ZEB1-AS1 could bind to IL-11 and promote IL-11 stability. Down-regulation of ZEB1-AS1 decreased IL-11 production of human bone marrow stromal cells (BMSCs), which led to suppressed proliferation and inhibited IL-11/STAT3 pathway in BALL-1 cells.

Conclusions

ZEB1-AS1 promotes the activation of IL-11/STAT3 signaling pathway by associating with IL-11 in B-ALL.

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Acknowledgements

This work was supported by Departmental sources.

Supporting information

Supplementary Fig. 1—Expressions of ZEB1 and ZEB1-AS1.

Supplementary Fig. 2—Effects of ZEB1-AS1 knockdown in BALL-1 cells.

Supplementary Fig. 3—Effects of ZEB1-AS1 knockdown in HS-5 cells.

Author information

Authors and Affiliations

  1. Department of Hematology, Guizhou Provincial People’s Hospital, Guiyang, 550002, Guizhou, China

    Qing Wang, Xiaoli Du, Min Yang, Shishan Xiao, Jun Cao, Jun Song & Linhui Wang

Authors
  1. Qing Wang
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  2. Xiaoli Du
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  3. Min Yang
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  4. Shishan Xiao
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  5. Jun Cao
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  6. Jun Song
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  7. Linhui Wang
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Corresponding author

Correspondence to Qing Wang.

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Conflict of interest

The authors declare no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the 1964 Helsinki Declaration and its later amendments.

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Cite this article

Wang, Q., Du, X., Yang, M. et al. LncRNA ZEB1-AS1 contributes to STAT3 activation by associating with IL-11 in B-lymphoblastic leukemia. Biotechnol Lett 39, 1801–1810 (2017). https://doi.org/10.1007/s10529-017-2421-3

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  • DOI: https://doi.org/10.1007/s10529-017-2421-3

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