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A prepared anti-MSTN polyclonal antibody reverses insulin resistance of diet-induced obese rats via regulation of PI3K/Akt/mTOR&FoxO1 signal pathways

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Abstract

Suppression of myostatin (MSTN) is associated with skeletal muscle atrophy and insulin resistance. However, the mechanisms by which MSTN regulates insulin resistance are not well known. We have explored the signaling pathways through which MSTN regulates insulin resistance in diet-induced obese rats using a polyclonal antibody for MSTN. The anti-MSTN polyclonal antibody significantly improved insulin resistance and whole-body insulin sensitivity, decreased MSTN protein expression in muscle samples by 39 % in diet-induced obese rats. Furthermore, the anti-MSTN polyclonal antibody significantly enhanced PI3K activity (140 %), Akt phosphorylation (86 %), GLUT4 protein expression (23 %), the phosphorylation of mTOR (21 %), and inhibited the phosphorylation of FoxO1 (57 %), but did not affect the phosphorylation of GSK-3β. Thus, suppression of MSTN by the anti-MSTN polyclonal antibody reverses insulin resistance of diet-induced obesity via MSTN/PI3K/Akt/mTOR and MSTN/PI3K/Akt/FoxO1 signaling pathways.

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Acknowledgments

This work was supported by the National Science Fund for Outstanding Young Investigators (30900710), the Natural Science Foundation of Shaanxi Province, China (2012JM3011), the Innovation Funds of Graduate Programs, SNNU (2012CXS036), and the Fundamental Research Funds for the Central Universities (GK201302042 and GK201402045). We would like to thank all the members of our laboratory for their encouragement and help with this study.

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The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Institute of Sports Biology, Shaanxi Normal University, Xi’an, 710062, China

    Liang Tang, Xu-dan Wang, Kai Luo, Di-di Zhang, Ai-ping Chi, Jing Zhang & Li-jun Sun

  2. Postdoctoral Research Station of Biology, Shaanxi Normal University, Xi’an, 710062, China

    Li-jun Sun

  3. Department of Physical Education, Northwest University, Xi’an, 710069, China

    Chen-tao Liu

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  1. Liang Tang
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  2. Chen-tao Liu
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  3. Xu-dan Wang
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  4. Kai Luo
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  5. Di-di Zhang
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  6. Ai-ping Chi
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Correspondence to Li-jun Sun.

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Tang, L., Liu, Ct., Wang, Xd. et al. A prepared anti-MSTN polyclonal antibody reverses insulin resistance of diet-induced obese rats via regulation of PI3K/Akt/mTOR&FoxO1 signal pathways. Biotechnol Lett 36, 2417–2423 (2014). https://doi.org/10.1007/s10529-014-1617-z

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  • DOI: https://doi.org/10.1007/s10529-014-1617-z

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