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Lycium barbarum polysaccharide fraction associated with photobiomodulation protects from epithelium thickness and collagen fragmentation in a model of cutaneous photodamage

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Abstract

Ultraviolet radiation (UVR) is the major etiologic agent of cutaneous photoaging, and different strategies are used to prevent and treat this condition. The polysaccharide fraction (LBPF) isolated from Lycium Barbarum fruits (goji berry) contains several active ingredients with antioxidant, immune system modulation, and antitumor effects. In addition, the photobiomodulation (PBM) is widely applied in photoaging treatment. This study investigated the effects of LBPF and PBM against the UVR-induced photodamage in the skin of hairless mice. The mice were photoaged for 6 weeks in a chronic and cumulative exposure regimen using a 300-W incandescent lamp that simulates the UVR effects. From the third to the sixth week of photoaging induction, the animals received topical applications of LBPF and PBM, singly or combined, in different orders (first LBPF and then PBM and inversely), three times per week after each session of photoaging. After completion of experiments, the dorsal region skin was collected for the analysis of thickness, collagen content, and metalloproteinases (MMP) levels. A photoprotective potential against the increase of the epithelium thickness and the fragmentation of the collagen fibers was achieved in the skin of mice treated with LBPF or PBM singly, as well as their combination. All treatments maintained the skin collagen composition, except when PBM was applied after the LBPF. However, no treatment protected against the UVR-induced MMP increase. Taken together, we have shown that the LBPF and PBM promote a photoprotective effect in hairless mice skin against epidermal thickening and low collagen density. Both strategies, singly and combined, can be used to reduce the UVR-induced cutaneous photoaging.

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The dataset supporting the results will be available upon request.

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Funding

This work was supported by FAPESP (São Paulo Research Foundation, grants nos. 2015/24940-8, 2013/27021-8, and 2012/25299-6), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Finance Code 001), and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).

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Authors and Affiliations

  1. Laboratory of Biology of Aging (LABEN), Department of Gerontology, Federal University of São Carlos, São Carlos, SP, CEP 13565-905, Brazil

    Lia Mara Grosso Neves & Marcia Regina Cominetti

  2. Postgraduate Program in Biomedical Engineering, Brazil University, São Paulo, SP, CEP 08230-030, Brazil

    Carla Roberta Tim & Nivaldo Antonio Parizotto

  3. Department of Pathology and Legal Medicine, University of São Paulo, Ribeirão Preto, SP, CEP 14049-900, Brazil

    Elaine Medeiros Floriano

  4. Department of Medicine, Federal University of São Carlos, São Carlos, SP, CEP 13565-905, Brazil

    Lucimar Retto da Silva de Avó

  5. Department of Chemistry, Federal University of São Carlos, São Carlos, SP, CEP 13565-905, Brazil

    João Batista Fernandes

  6. Joint Graduate Program in Physical Therapy, Federal University of São Carlos, São Carlos, SP, CEP 13565-905, Brazil

    Nivaldo Antonio Parizotto

  7. Postgraduate Program in Biotechnology in Regenerative Medicine and Medical Chemistry, University of Araraquara, Araraquara, SP, CEP 14801-340, Brazil

    Nivaldo Antonio Parizotto

Authors
  1. Lia Mara Grosso Neves
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  2. Carla Roberta Tim
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  3. Elaine Medeiros Floriano
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  4. Lucimar Retto da Silva de Avó
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  5. João Batista Fernandes
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  6. Nivaldo Antonio Parizotto
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  7. Marcia Regina Cominetti
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Contributions

LMG: conception and design, data acquisition, data analyses and interpretation, review of the final version; CRT: data acquisition, review of the final version; EMF: data interpretation, review of the final version; LRSA: data interpretation, review of the final version; JBF: data interpretation, review of the final version; NAP: conception and design, data analyses and interpretation, review of the final version; MRC: conception and design, data acquisition, funding acquisition, data analyses and interpretation, review of the final version

Corresponding authors

Correspondence to Lia Mara Grosso Neves or Marcia Regina Cominetti.

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The authors declare that they have no conflicts of interest.

Ethics approval

This study was carried out in strict accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Committee on the Ethics of Animal Experiments (information excluded for the sake of animosity).

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Neves, L.M.G., Tim, C.R., Floriano, E.M. et al. Lycium barbarum polysaccharide fraction associated with photobiomodulation protects from epithelium thickness and collagen fragmentation in a model of cutaneous photodamage. Lasers Med Sci 36, 863–870 (2021). https://doi.org/10.1007/s10103-020-03132-w

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  • DOI: https://doi.org/10.1007/s10103-020-03132-w

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