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Progression of erosive lesions after Nd:YAG laser and fluoride using optical coherence tomography

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Abstract

This study aimed to use optical coherence tomography (OCT) to assess the progression of erosive lesions after irradiation with Nd:YAG laser and application of topical fluoride. One-hundred and twenty dentin samples (4 × 4 × 2 mm) obtained from bovine incisors were used. Samples were protected with acid-resistant nail varnish, with exception of a central circular area 2 mm in diameter. All samples were submitted to erosive cycles with citric acid solution 0.05 M (citric acid monohydrate—C6H8O7·H2O); M = 210.14 g/mol) pH 2.3, at room temperature, for 20 min, 2×/day, throughout 20 days. After 10 days of acid challenges, lesions became visible, and each group received a different treatment (n = 15): control (without treatment), topical application of sodium fluoride 2 % for 4 min; Nd:YAG laser with different irradiation parameters (1, 0.7, and 0.5 W); and the association of fluoride with the laser parameters. OCT readouts were performed on day 01 (before the first acid challenge—OCT1), on day 05 (OCT2), day 10 (OCT3—after treatment), day 15 (OCT4), day 17 (OCT5), and day 20 (OCT6). The OCT images generated made it possible to measure the amount of tooth tissue loss over the 20 days of erosive cycle, before and after treatments, and to monitor early dentin demineralization progression. After statistical analysis, the fluoride group was observed to be the one that showed smaller loss of tissue over time. The OCT technique is promising for diagnosing and monitoring erosive lesion damage; however, further in vitro and in vivo research is needed to improve its use.

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Acknowledgments

The authors would like to thank the Special Laboratory of Lasers in Dentistry (LELO) of the School of Dentistry of University of São Paulo and the Center for Laser Applications (CLA) at the Institute of Energy and Nuclear Research for allowing the implementation of this study. The authors would aso like to thank FAPESP (São Paulo Research Foundation) and CNPq (National Counsel of Technological and Scientific Development) for supporting the research.

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Authors and Affiliations

  1. Institute of Energetic and Nuclear Research, IPEN-CNEN/SP, Av. Prof. Lineu Prestes, 2242–Cidade Universitária, CEP 05508-000, São Paulo, Brazil

    Marcia Cristina Dias de Moraes & Anderson Zanardi Freitas

  2. Special Laboratory of Lasers in Dentistry (LELO), Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Professor Lineu Prestes 2227–Cidade Universitária, CEP 05508-000, São Paulo, SP, Brazil

    Ana Cecilia Correa Aranha

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  1. Marcia Cristina Dias de Moraes
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  2. Anderson Zanardi Freitas
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  3. Ana Cecilia Correa Aranha
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Correspondence to Ana Cecilia Correa Aranha.

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The authors declare that they have no conflict of interest. Authors disclose all relationships or interests that could have direct or potential influence or impart bias on the work.

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The manuscript has not been submitted to more than LIMS for simultaneous consideration. The material has not been published previously (partly or in full). This study is not split up into several parts to increase the quantity of submissions and submitted to various journals or to one journal over time. No data have been fabricated or manipulated (including images) to support our conclusions.All authors agree to submit, and they have explicitly signed an agreement document. Authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results.

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de Moraes, M.C.D., Freitas, A.Z. & Aranha, A.C.C. Progression of erosive lesions after Nd:YAG laser and fluoride using optical coherence tomography. Lasers Med Sci 32, 1–8 (2017). https://doi.org/10.1007/s10103-016-2075-8

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  • DOI: https://doi.org/10.1007/s10103-016-2075-8

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