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Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

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Abstract

Lasers emit light beams with specific characteristics, in which wavelength, frequency, power, fluence, and emission mode properties determine the photophysical, photochemical, and photobiological responses. Low-intensity lasers could induce free radical generation in biological tissues and cause alterations in macromolecules, such as DNA. Thus, the aim of this work was to evaluate excision repair cross-complementing group 1 (ERCC1) and excision repair cross-complementing group 2 (ERCC2) messenger RNA (mRNA) expression in biological tissues exposed to low-intensity lasers. Wistar rat (n = 28, 4 for each group) skin and muscle were exposed to low-intensity red (660 nm) and near-infrared (880 nm) lasers at different fluences (25, 50, and 100 J/cm2), and samples of these tissues were withdrawn for RNA extraction, cDNA synthesis, and gene expression evaluation by quantitative polymerase chain reaction. Laser exposure was in continuous wave and power of 100 mW. Data show that ERCC1 and ERCC2 mRNA expressions decrease in skin (p < 0.001) exposed to near-infrared laser, but increase in muscle tissue (p < 0.001). ERCC1 mRNA expression does not alter (p > 0.05), but ERCC2 mRNA expression decreases in skin (p < 0.001) and increases in muscle tissue (p < 0.001) exposed to red laser. Our results show that ERCC1 and ERCC2 mRNA expression is differently altered in skin and muscle tissue exposed to low-intensity lasers depending on wavelengths and fluences used in therapeutic protocols.

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Acknowledgments

This work was supported by the Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), and Conselho Nacional de Pesquisa (CNPq).

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Authors and Affiliations

  1. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro, 20550900, Brazil

    Luiz Philippe S. Sergio, Vera Maria A. Campos, Andre Luiz Mencalha & Adenilson S. Fonseca

  2. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036900, Brazil

    Flavia de Paoli

  3. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca 94, Rio de Janeiro, 20211040, Brazil

    Solange C. Vicentini & Adenilson S. Fonseca

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  1. Luiz Philippe S. Sergio
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  2. Vera Maria A. Campos
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  3. Solange C. Vicentini
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  4. Andre Luiz Mencalha
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  5. Flavia de Paoli
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  6. Adenilson S. Fonseca
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Correspondence to Adenilson S. Fonseca.

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Experiments were conducted in accordance with the Institutional Committee of Animal Care (Comissão de Ética para o Cuidado e Uso de Animais Experimentais, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro), protocol CEUA/038/2012.

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Sergio, L.P.S., Campos, V.M.A., Vicentini, S.C. et al. Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue. Lasers Med Sci 31, 429–435 (2016). https://doi.org/10.1007/s10103-016-1870-6

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  • DOI: https://doi.org/10.1007/s10103-016-1870-6

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