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Effect of IR laser photobiomodulation on the repair of bone defects grafted with organic bovine bone

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Abstract

A major problem on modern dentistry is the recovery of bone defects of different etiologies. Biomaterials are used to improve the repair of these defects. Previous studies have shown positive effects of Laser Photobiomodulation (LPBM) on the repair of both soft and bone tissues. This study assessed histologically the effect of LPBM on the repair of surgical defects on the femur of rats filled with lyophilized bovine bone. The animals were divided into three groups: group I (control); group II (graft); group III (graft + LPBM). The animals on the irradiated groups received 16 J/cm2 per session divided into four points around the defect being the first irradiation immediately after surgery and repeated at every 48 h during 2 weeks. Animal death occurred 15, 21, and 30 days after surgery. The specimens were routinely processed and stained with H/E and Sirius red and analyzed by light microscopy. There was histological evidence of improved collagen fiber deposition at early stages of the healing; increased amount of well-organized bone trabeculae at the end of the experimental period on irradiated animals. It is concluded that LPBM has positive biomodulative effect on the healing process bone defects.

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Acknowledgments

The authors would like to thanks the Conselho Nacional de Desensenvolvimento Tecnológico—CNPq for the Grant that allowed the realization of this work.

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Authors and Affiliations

  1. School of Dentistry, Pernambuco University, Camaragibe, Pernambuco, Brazil

    Marleny Elizabeth Márquez Martínez

  2. Laser Center, School of Dentistry, Department of Propedeutica and Clínica Integrada, Universidade Federal da Bahia, Salvador, Brazil

    Antonio Luiz Barbosa Pinheiro

  3. School of Dentistry, Department of Propedeutica and Clínica Integrada, Federal University of Bahia, Salvador, Brazil

    Luciana Maria Pedreira Ramalho

  4. Laser Center, Faculdade de Odontologia, Universidade Federal da Bahia, Av. Araújo Pinho, 62, Canela, Salvador, Bahia, CEP 40140-110, Brazil

    Antonio Luiz Barbosa Pinheiro

Authors
  1. Marleny Elizabeth Márquez Martínez
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  2. Antonio Luiz Barbosa Pinheiro
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  3. Luciana Maria Pedreira Ramalho
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Correspondence to Antonio Luiz Barbosa Pinheiro.

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Márquez Martínez, M.E., Pinheiro, A.L.B. & Ramalho, L.M.P. Effect of IR laser photobiomodulation on the repair of bone defects grafted with organic bovine bone. Lasers Med Sci 23, 313–317 (2008). https://doi.org/10.1007/s10103-007-0488-0

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  • DOI: https://doi.org/10.1007/s10103-007-0488-0

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