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Double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer

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Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objectives

The aim of the present study is to evaluate the possible correlation between sealer penetration into dentinal tubules and sealing ability both in presence and absence of smear layer.

Materials and methods

Fourteen maxillary central incisors were treated with 5.25 % NaOCl +10 % EDTA to remove the smear layer (SL-free group) or 5.25 % NaOCl without EDTA (SL group). Root canals were filled using #25 Thermafil Obturators with Topseal sealer labelled with 0.1 wt% rhodamine B. Sealing ability was measured as fluid filtration rate with a fluid-flow meter using water supplemented with 0.3 % calcein fluorescent dye. Specimens were sectioned, observed under confocal microscope to co-localize the presence of sealer (rhodamine B labelling) into dentinal tubules and gaps (calcein labelling) into the root canal. The depth of sealer penetration into dentinal tubules and the percentage of sealer penetration around the root canal were measured at 3, 5 and 8 mm from the apex.

Results

No significant differences between groups were observed in fluid filtration rate nor in depth of calcein penetration. Sealer penetration depth and percentage into dentinal tubules were not significantly different between groups, except at 8-mm level in absence of smear layer.

Conclusion

Sealer penetration at 3- and 5-mm levels was not influenced by smear layer while it was significantly reduced at 8-mm level. Fluid filtration rate was not correlated either with depth of calcein penetration nor with sealer penetration into dentinal tubules.

Clinical relevance

The sealing ability of Topseal sealer is not affected by presence or absence of smear layer.

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Authors and Affiliations

  1. Department of Surgery, Medicine, Dentistry and Morphological Sciences with Transplant Surgery, Oncology and Regenerative Medicine Relevance, School of Dentistry, Endodontic Section, University of Modena and Reggio Emilia, Modena, Italy

    Luigi Generali

  2. Endodontic Clinical Section, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy

    C. Prati & C. Pirani

  3. Master Program in Clinical Endodontic, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy

    C. Prati

  4. Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia, Modena, Italy

    F. Cavani

  5. Laboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy

    M. R. Gatto & M. G. Gandolfi

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  1. Luigi Generali
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  2. C. Prati
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  3. C. Pirani
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  5. M. R. Gatto
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  6. M. G. Gandolfi
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Correspondence to Luigi Generali.

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The authors declare that they have no conflict of interest.

Funding

The work was supported by the Department of Surgery, Medicine, Dentistry and Morphological Sciences with Transplant Surgery, Oncology and Regenerative Medicine Relevance, University of Modena and Reggio Emilia, Modena, Italy.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Generali, L., Prati, C., Pirani, C. et al. Double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer. Clin Oral Invest 21, 1267–1276 (2017). https://doi.org/10.1007/s00784-016-1878-0

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  • DOI: https://doi.org/10.1007/s00784-016-1878-0

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