Summary.
Taurine influences bone metabolism and is taken up by cells via a specific transport system, the taurine transporter (TAUT). We report a link between taurine and bone homeostasis by demonstrating transcription and translation of TAUT in bone-forming cells. TAUT was expressed in human primary osteoblasts, the human osteosarcoma osteoblast-like cell line MG63, and the mouse osteoblastic cell line MC3T3-E1. Immunostaining with polyclonal antibodies also demonstrated the presence of TAUT in both human and murine osteoblasts. TAUT mRNA expression and [3H]taurine uptake increased during differentiation of MG63 cells in culture. Supplementation of culture medium with taurine enhanced alkaline phosphatase activity and osteocalcin secretion. The regulation and detailed function of taurine and TAUT in bone remain unclear, but our findings suggest a functional role for them in bone homeostasis.
Similar content being viewed by others
References
RG Erben (1997) ArticleTitleEmbedding of bone samples in methylmethacrylate: an improved method suitable for bone histomorphometry, histochemistry, and immunohistochemistry J Histochem Cytochem 45 307–313 Occurrence Handle9016319 Occurrence Handle1:CAS:528:DyaK2sXhvVylsLk%3D
J Hillenkamp AA Hussain TL Jackson JR Cunningham J Marshall (2004) ArticleTitleTaurine uptake by human retinal pigment epithelium implications for the transport of small solutes between the choroid and the outer retina Invest Ophthalmol Vis Sci 45 4529–4534 Occurrence Handle15557464 Occurrence Handle10.1167/iovs.04-0919
RJ Huxtable (1992) ArticleTitlePhysiological actions of taurine Physiol Rev 72 101–163 Occurrence Handle1731369 Occurrence Handle1:CAS:528:DyaK38XhsVKhsLw%3D
SM Jhiang L Fithian P Smanik J McGill Q Tong EL Mazzaferri (1993) ArticleTitleCloning of the human taurine transporter and characterization of taurine uptake in thyroid cells FEBS Lett 318 139–144 Occurrence Handle8382624 Occurrence Handle1:CAS:528:DyaK3sXisFaisL0%3D Occurrence Handle10.1016/0014-5793(93)80008-I
M Koide N Okahashi R Tanaka K Kazuno K Shibasaki Y Yamazaki K Kaneko N Ueda M Ohguchi Y Ishihara T Noguchi T Nishihara (1999) ArticleTitleInhibition of experimental bone resorption and osteoclast formation and survival by 2-aminoethanesulphonic acid Arch Oral Biol 44 711–719 Occurrence Handle10471155 Occurrence Handle1:CAS:528:DyaK1MXlvVOku7g%3D Occurrence Handle10.1016/S0003-9969(99)00067-9
EY Liao XH Luo (2001) ArticleTitleEffects of 17beta-estradiol on the expression of matrix metalloproteinase-1, -2 and tissue inhibitor of metalloproteinase-1 in human osteoblast-like cell cultures Endocrine 15 291–295 Occurrence Handle11762702 Occurrence Handle1:CAS:528:DC%2BD3MXovFOiu7Y%3D Occurrence Handle10.1385/ENDO:15:3:291
H Lill N Nelson (1998) ArticleTitleHomologies and family relationships among Na+/Cl− neurotransmitter transporters Methods Enzymol 296 425–436 Occurrence Handle9779464 Occurrence Handle1:CAS:528:DyaK1MXltVej Occurrence Handle10.1016/S0076-6879(98)96030-X
QR Liu B Lopez-Corcuera H Nelson S Mandiyan N Nelson (1992) ArticleTitleCloning and expression of a cDNA encoding the transporter of taurine and beta-alanine in mouse brain Proc Natl Acad Sci USA 89 12145–12149 Occurrence Handle1465453 Occurrence Handle1:CAS:528:DyaK3sXkvV2huro%3D Occurrence Handle10.1073/pnas.89.24.12145
XH Luo EY Liao X Su (2002) ArticleTitleProgesterone upregulates TGF-b isoforms (b1, b2, and b3) expression in normal human osteoblast-like cells Calcif Tissue Int 71 329–334 Occurrence Handle12154395 Occurrence Handle1:CAS:528:DC%2BD38XovFOqt7s%3D Occurrence Handle10.1007/s00223-001-2129-0
Y Miyamoto GI Liou TJ Sprinkle (1996) ArticleTitleIsolation of a cDNA encoding a taurine transporter in the human retinal pigment epithelium Curr Eye Res 15 345–349 Occurrence Handle8654117 Occurrence Handle1:STN:280:DyaK283lsVyrsw%3D%3D
S Park H Kim SJ Kim (2001) ArticleTitleStimulation of ERK2 by taurine with enhanced alkaline phosphatase activity and collagen synthesis in osteoblast-like UMR-106 cells Biochem Pharmacol 62 1107–1111 Occurrence Handle11597579 Occurrence Handle1:CAS:528:DC%2BD3MXntlyjsbk%3D Occurrence Handle10.1016/S0006-2952(01)00741-9
H Pasantes-Morales O Quesada J Moran (1998) ArticleTitleTaurine: An osmolyte in mammalian tissues Adv Exp Med Biol 442 209–217 Occurrence Handle9635034 Occurrence Handle1:CAS:528:DyaK1cXls1Wrtbc%3D
KA Poulsen T Litman J Eriksen J Mollerup IH Lambert (2002) ArticleTitleDownregulation of taurine uptake in multidrug resistant Ehrlich ascites tumor cells Amino Acids 22 333–350 Occurrence Handle12107761 Occurrence Handle1:CAS:528:DC%2BD38XmvFCqt7w%3D Occurrence Handle10.1007/s007260200019
X Qian S Vinnakota C Edwards HK Sarkar (2000) ArticleTitleMolecular characterization of taurine transport in bovine aortic endothelial cells Biochim Biophys Acta 1509 324–334 Occurrence Handle11118543 Occurrence Handle1:CAS:528:DC%2BD3cXoslGis7Y%3D Occurrence Handle10.1016/S0005-2736(00)00315-1
S Ramamoorthy FH Leibach VB Mahesh H Han T Yang Feng RD Blakely V Ganapathy (1994) ArticleTitleFunctional characterization and chromosomal localization of a cloned taurine transporter from human placenta Biochem J 300 893–900 Occurrence Handle8010975 Occurrence Handle1:CAS:528:DyaK2cXlslWkt70%3D
S Ramamoorthy MA Del Monte FH Leibach V Ganapathy (1994) ArticleTitleMolecular identity and calmodulin-mediated regulation of the taurine transporter in a human retinal pigment epithelial cell line Curr Eye Res 13 523–529 Occurrence Handle7924416 Occurrence Handle1:STN:280:DyaK2M%2FgvV2jsA%3D%3D
S Roos TL Powell T Jansson (2004) ArticleTitleHuman placental taurine transporter in uncomplicated and IUGR pregnancies: cellular localization, protein expression, and regulation Am J Physiol Regul Integr Comp Physiol 287 R886–R893 Occurrence Handle15166008 Occurrence Handle1:CAS:528:DC%2BD2cXptF2rsLo%3D
KE Smith LA Borden CH Wang PR Hartig TA Branchek RL Weinshank (1992) ArticleTitleCloning and expression of a high affinity taurine transporter from rat brain Mol Pharmacol 42 563–569 Occurrence Handle1435737 Occurrence Handle1:CAS:528:DyaK3sXkvV2hsrk%3D
A Terauchi A Nakazaw K Johkura L Yan N Usuda (1998) ArticleTitleImmunohistochemical localization of taurine in various tissues of the mouse Amino Acids 15 151–160 Occurrence Handle9871495 Occurrence Handle1:CAS:528:DyaK1cXmvVGks7Y%3D Occurrence Handle10.1007/BF01345288
S Uchida HM Kwon A Yamauchi AS Preston F Marumo JS Handler (1992) ArticleTitleMolecular cloning of the cDNA for an MDCK cell Na(+)- and Cl(−)-dependent taurine transporter that is regulated by hypertonicity Proc Natl Acad Sci USA 89 8230–8234 Occurrence Handle1518851 Occurrence Handle1:CAS:528:DyaK3sXksVemt7c%3D Occurrence Handle10.1073/pnas.89.17.8230
S Vinnakota X Qian H Egal V Sarthy HK Sarkar (1997) ArticleTitleMolecular characterization and in situ localization of a mouse retinal taurine transporter J Neurochem 69 2238–2250 Occurrence Handle9375654 Occurrence Handle1:CAS:528:DyaK2sXntlKmu74%3D Occurrence Handle10.1046/j.1471-4159.1997.69062238.x
JW Voss SF Pedersen ST Christensen IH Lambert (2004) ArticleTitleRegulation of the expression and subcellular localization of the taurine transporter TAUT in mouse NIH3T3 fibroblasts Eur J Biochem 271 4646–4658 Occurrence Handle15606752 Occurrence Handle1:CAS:528:DC%2BD2MXjvFCmtQ%3D%3D Occurrence Handle10.1111/j.1432-1033.2004.04420.x
CE Wright HH Tallan YY Lin GE Gaull (1986) ArticleTitleTaurine: biological update Annu Rev Biochem 55 427–453 Occurrence Handle3527049 Occurrence Handle1:CAS:528:DyaL28XlsVyhsbs%3D Occurrence Handle10.1146/annurev.bi.55.070186.002235
YX Xu A Wagenfeld CH Yeung W Lehnert TG Cooper (2003) ArticleTitleExpression and location of taurine transporters and channels in the epididymis of infertile c-ros receptor tyrosine kinase-deficient and fertile heterozygous mice Mol Reprod Dev 64 144–151 Occurrence Handle12506346 Occurrence Handle1:CAS:528:DC%2BD3sXis1ymtg%3D%3D Occurrence Handle10.1002/mrd.10250
C Yasutomi H Nakamuta T Fujita T Takenaga M Koida (2002) ArticleTitleAnti-osteopenic effect of taurine: possible involvement of activated MEK-ERK-Cbfa1 signaling Nippon Yakurigaku Zasshi 120 114P–115P Occurrence Handle12491800
Author information
Authors and Affiliations
Additional information
The first two authors contributed equally to this work.
Rights and permissions
About this article
Cite this article
Yuan, LQ., Xie, H., Luo, XH. et al. Taurine transporter is expressed in osteoblasts. Amino Acids 31, 157–163 (2006). https://doi.org/10.1007/s00726-005-0313-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00726-005-0313-7