Abstract
Feline immunodeficiency virus (FIV), genus Lentivirus, is responsible for feline immunodeficiency syndrome in domestic cats. FIV has been classified into six subtypes: A, B, C, D, E and F, based on regions of the env gene as well as the gag gene. In Argentina, the circulation of subtypes B and E was reported more than two decades ago. The objective of this work was to study the FIV variants circulating presently in the city of Buenos Aires in naturally infected cats utilizing a nested PCR targeting the gag gene. A phylogenetic comparison with representative sequences of five previously published subtypes shows a clustering with subtypes A and B. This is the first report of FIV subtype A in Argentina.
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Acknowledgements
We thank Dr. Fiorella Kotsias for reviewing the manuscript, Dr. Danilo Bucafusco for helping with RDPv4.95 software, and Dr. Graciela Marrube for providing the housekeeping ABCB1 gene primers used in the control PCRs.
Funding
This work was funded by SECyT, University of Buenos Aires, cod. UBACyT2016 ModII-20020150200159BA.
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705_2019_4363_MOESM1_ESM.eps
Supplementary material 1 (EPS 4577 kb) Online Resource 1 Alignment of p24 amino acid sequences utilized for comparison in this work
705_2019_4363_MOESM2_ESM.eps
Supplementary material 2 (EPS 6601 kb) Online Resource 2 Table showing differences in amino acid sequences between Argentinian sequences and European and American sequences
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Huguet, M., Novo, S.G. & Bratanich, A. Detection of feline immunodeficiency virus subtypes A and B circulating in the city of Buenos Aires. Arch Virol 164, 2769–2774 (2019). https://doi.org/10.1007/s00705-019-04363-1
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DOI: https://doi.org/10.1007/s00705-019-04363-1