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Molecular detection and characterization of genotype 1 bovine leukemia virus from beef cattle in the traditional sector in Zambia

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Abstract

Whilst bovine leukemia virus (BLV) causes considerable economic losses to the dairy industry worldwide, information on its molecular epidemiology and economic impact in beef cattle is limited. Here, blood from 880 animals from Zambia’s major cattle-rearing provinces was screened for BLV by nested PCR. Positive pools were sequenced and phylogenetically analyzed. The estimated pooled prevalence was 2.1%. All strains belonged to genotype 1 and formed a distinct phylogenetic cluster. The study suggests circulation of genotype 1 BLV in beef cattle in these regions. This is the first report on molecular detection and characterization of BLV from beef cattle in Africa.

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Acknowledgements

This work was supported by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) and the Japan Agency for Medical Research and Development (AMED)/Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS).

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Authors and Affiliations

  1. School of Natural Sciences, The University of Zambia, PO Box 32379, Lusaka, 10101, Zambia

    Mundia M. Phiri & Evans Kaimoyo

  2. Department of Disease Control, School of Veterinary Medicine, The University of Zambia, PO Box 32379, Lusaka, 10101, Zambia

    Katendi Changula, Isaac Silwamba, Penjaninge Kapila, Martin Simuunza, John Bwalya Muma, Girja S. Pandey, Ayato Takada, Aaron S. Mweene & Edgar Simulundu

  3. Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan

    Herman M. Chambaro, Masahiro Kajihara & Ayato Takada

  4. Africa Center of Excellence of Infectious Diseases of Humans and Animals, University of Zambia, Lusaka, Zambia

    Martin Simuunza & Aaron S. Mweene

  5. School of Health Sciences, The University of Zambia, PO Box 50110, Lusaka, 10101, Zambia

    Simbarashe Chitanga

Authors
  1. Mundia M. Phiri
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  2. Evans Kaimoyo
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  3. Katendi Changula
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  4. Isaac Silwamba
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  13. Simbarashe Chitanga
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  14. Edgar Simulundu
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Correspondence to Edgar Simulundu.

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Phiri, M.M., Kaimoyo, E., Changula, K. et al. Molecular detection and characterization of genotype 1 bovine leukemia virus from beef cattle in the traditional sector in Zambia. Arch Virol 164, 2531–2536 (2019). https://doi.org/10.1007/s00705-019-04350-6

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  • DOI: https://doi.org/10.1007/s00705-019-04350-6

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