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Characterization and genomic analyses of Aeromonas hydrophila phages AhSzq-1 and AhSzw-1, isolates representing new species within the T5virus genus

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Abstract

In this study, two bacteriophage isolates, AhSzq-1 and AhSzw-1 that specifically infect Aeromonas hydrophila strain KT998822, were isolated from seawater and characterized. One-step growth curves showed that the latent period of AhSzq-1 and AhSzw-1 are 50 min and 60 min, respectively. The sequence similarities between AhSzq-1 and AhSzw-1 were 88% at the DNA and 83% at the protein level, suggesting that these two phages are representatives of two different species. The virion morphology, DNA genome size and terminal repeats of these two phages are similar to those of viruses classified as T5virus phages. Both phylogenetic analyses and proteomic comparison show that AhSzq-1 and AhSzw-1 group with members of the T5virus genus. We thus propose these two phages as representative isolates of two new species within the T5virus genus.

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Acknowledgements

We are grateful to J. Feng from Institute of Microbiology of the Chinese Academy of Sciences for providing bacterial strains. This study was funded by the Shenzhen Science and Technology Innovation Committee (JCYJ20160229201759414, JCYJ20160122143446357, JSGG20150229145252927, JCYJ20160612152651093, JCYJ20140901003939019, JCYJ20150629151046896 and CXZZ20140901004122088), the Shenzhen Peacock Team Project (KQTD2015033117210153 and KQTD2016112915000294), and the Guangdong Science and Technology Department (2014A020216029). This work was also funded by the National Natural Science Foundation of China (31570115, Y520581001), and National Basic Research Program of China (973 Program: 2014CB745202).

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Authors and Affiliations

  1. Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518000, China

    Shengjian Yuan, Ling Chen, Yan Zhou & Yingfei Ma

  2. Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, 518000, China

    Quan Liu

  3. R&D Center, Shenzhen Alpha Feed Co., Ltd, Shenzhen, 518000, China

    Jingfang Yang & Deng Deng

  4. Medical Institute for Children and Women, Shenzhen Baoan Maternal and Child Health Hospital, Shenzhen, 518000, China

    Hongping Li

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  1. Shengjian Yuan
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Correspondence to Yingfei Ma.

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Yuan, S., Chen, L., Liu, Q. et al. Characterization and genomic analyses of Aeromonas hydrophila phages AhSzq-1 and AhSzw-1, isolates representing new species within the T5virus genus. Arch Virol 163, 1985–1988 (2018). https://doi.org/10.1007/s00705-018-3805-y

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  • DOI: https://doi.org/10.1007/s00705-018-3805-y

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