Abstract
In cases of lower rectum-located tumor or severe disease, surgical resection is currently the effective management; however, it also carries increased risks of function loss. The interdisciplinary field of regenerative medicine offers strategies that can potentially restore severely diseased and injured tissues and organs. Adipose-derived stromal cells (ASCs) are an abundant and accessible source of adult stem cells and hold great promise as therapeutic agents for tissue regeneration. In this work, we transplanted cells isolated from human stromal tissues, including a 6%–7% ASC population, into heat-damaged femoral muscles of non-obese diabetic immunodeficient mice. The movement of the limbs was observed to determine the functional recovery 3 months after transplantation. Among the mice that did not receive cell transplantation, 20% were able to walk with the injured limb touching the ground, while all of the mice in the ASC-treatment group were able to walk. Furthermore, all ASC-treated mice were able to stand on both back paws, in contrast to the control group mice. The human stromal cell population containing ASCs was able to differentiate and engraft the injured muscle tissues successfully. Our results indicate that stromal material/ASC-based therapies are a promising strategy for the regeneration of tissues and function restoration after severe injury due to surgery.
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Online Resource 1. Mice were placed in a cage without sawdust, and the movements of the legs were evaluated to determine whether the previously damaged limbs could be used for walking or standing. In the control group, one of the four mice was able to walk using its damaged leg, and none these mice were able to stand on both back paws. In contrast, all of the mice in the ASC-treatment group were able to walk using their four limbs, including the heels, and all were able to stand on both back paws as well
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Miyoshi, N., Fujino, S., Takahashi, Y. et al. Implantation of human adipose-derived stromal cells for the functional recovery of a murine heat-damaged muscle model. Surg Today 50, 1699–1706 (2020). https://doi.org/10.1007/s00595-020-02026-2
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DOI: https://doi.org/10.1007/s00595-020-02026-2