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Safety assessment of aqueous acetone extract of Clerodendrum infortunatum L. in Wistar rats

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Abstract

Clerodendrum infortunatum L. is a traditional medicinal plant used for the treatment of bronchitis, malarial fever, benign tumour etc. Antioxidant screening revealed that the aqueous acetone extract exhibited the greatest antioxidant activity. In the present study, toxicity analysis of the aqueous acetone extract was carried out in male Wistar rats to evaluate its impact on the liver and serum biochemical parameters. Male Wistar rats were divided into six groups and groups II to VI were treated with a single dose of 250, 500, 1000 and 2000 mg/kg body weight of aqueous acetone extract and silymarin, respectively. Group I served as the normal control. The rats were observed for 14 days. At the end, the blood was collected for the estimation of aspartate transaminase, alanine transaminase, gamma glutamate transaminase, alkaline phosphatase, lactate dehydrogenase, direct and total bilirubin, albumin, globulin, A/G ratio and total protein. Antioxidant parameters such as superoxide dismutase, catalase, reduced glutathione, lipid peroxidation and histopathological examination were carried out in liver tissue. All the biochemical parameters were found to be normal. Results suggest that the administration of aqueous acetone extract of C. infortunatum was safe and can be used as a new source of drug for the modern pharmaceutics.

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References

  • Adedapo AA, Abatan MO, Olorunsogo OO (2004) Toxic effects of some plants in the genus Euphorbia on haematological and biochemical parameters of rats. VetArhiv 74:53–62

    Google Scholar 

  • Alagammal M, Lincy MP, Mohan VR (2013) Hepatoprotective and antioxidant effect of Polygala rosmarinifolia Wight & Arn against CCl4 induced hepatotoxicity in rats. J Pharmacogn Phytochemistry 2:118–124

    Google Scholar 

  • Aruoma OI (1998) Free radicals, oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc 75:199–212

    Article  CAS  Google Scholar 

  • Azeez OM, Akhigbe RE, Anigbogu CN (2013) Oxidative status in rats kidney exposed to petroleum hydrocarbons. J Nat Sci Biol Med 4:149–154

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bandaranayake WM (2006) Quality control, screening, toxicity and regulation of herbal drugs. In:Ahmad I, Aqil F, Owais M (eds) Modern phytomedicine: Turning medicinal plants into drugs, 1st edn. Wiley-VCH Verlag GmbH and Co, Weinheim, pp 25–57

  • Deavall DG, Martin EA, Horner JM, Roberts R (2012) Drug induced oxidative stress and toxicity. J Toxicol 2012:645460

    Article  PubMed  PubMed Central  Google Scholar 

  • Dixit N, Baboota S, Kohli K, Ahmad S, Ali J (2007) Silymarin: a review of pharmacological aspects and bioavailability enhancement approaches. Indian J Pharmacol 39:172–179

    Article  CAS  Google Scholar 

  • Dooley JS (2011) Gallstones and benign biliary diseases. In: Dooley JS, Lok ASF, Burroughs AK, Heathcote EJ (eds) Sherlock’s diseases of the liver and biliary system, 12th edn. Blackwell Publishing, Chichester, pp 257–293

  • Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB (2000) Diagnosis and monitoring of hepatic injury. II. Recommendations for use of laboratory tests in screening, diagnosis, and monitoring. Clin Chem 46:2050–2068

    CAS  PubMed  Google Scholar 

  • Dzoyem JP, Nkegoum B, Kuete V (2013) A 4-week repeated oral dose toxicity study of the methanol extract from Diospyros canaliculata in rats. Comp Clin Pathol 22:75–81

    Article  CAS  Google Scholar 

  • Dzoyem JP, Kuete V, Eloff JN (2014) Biochemical parameters in toxicological studies in Africa: significance, principle of methods, data interpretation, and use in plant screenings. In: Kuete V (ed) Toxicological survey of African medicinal plants, 1st edn. Elsevier, London, pp 659–703

    Chapter  Google Scholar 

  • Ige SF, Akhigbe RE, Edeogho O, Ajao FO, Owolabi OQ, Oyekunle OS et al (2011) Hepatoprotective activities of Allium cepa in cadmium treated rats. Int J Pharm Pharm Sci 3:60–63

    Google Scholar 

  • Kalita P, Barman TK, Pal TK (2013) Estimation of total flavonoids content (tfc) and antioxidant activities of methanolic root extract of Clerodendrum infortunatum Linn. J Harmonized Res Pharm 2:91–99

    CAS  Google Scholar 

  • Kavitha P, Ramesh R, Bupesh G, Stalin A, Subramanian P (2011) Hepatoprotective activity of Tribulus terrestris extract against acetaminophen induced toxicity in a freshwater fish (Oreochromis mossambicus). In Vitro Cell Dev Biol Anim 47:698–706

    Article  CAS  PubMed  Google Scholar 

  • Khan RA, Khan MR, Sahreen S (2013) Attenuation of CCl4 induced hepatic oxidative stress in rat by Launaea procumbens. Exp Toxicol Pathol 65:319–326

    Article  CAS  PubMed  Google Scholar 

  • Khare CP (2007) Indian medicinal plants—an illustrated dictionary. Springer, Berlin

  • Leena PN, Aleykutty NA (2012) Comparitive study on antibacterial activities of Clerodendron infortunatum Linn and Clerodendron paniculatum Linn root extract. Int J Adv Pharm Biol Chem 1:325–327

    Google Scholar 

  • Li S, Tan H, Wang N, Zhang Z, Lao L, Wong C, Feng Y (2015) The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci 16:26087–26124

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mandade R, Sreenivas SA, Sakarkar DM, Choudhury A (2011) Radical scavenging and antioxidant activity of Hibiscus rosasinensis extract. Afr J Pharm Pharmacol 5:2027–2034

    Google Scholar 

  • Marconato L, Crispino G, Finotello R, Mazzotti S, Salerni F, Zini E (2009) Serum lactate dehydrogenase activity in canine malignancies. Vet Comp Oncol 7:236–243

    Article  CAS  PubMed  Google Scholar 

  • Marshall WJ (1992) Illustrated textbook of clinical chemistry. Gower Medical Publishing, London

    Google Scholar 

  • Nasri H, Shirzad H (2013) Toxicity and safety of medicinal plants. J HerbMed Plarmacol 2:21–22

    Google Scholar 

  • OECD (2011) OECD guidelines for the testing of chemicals (No. 423) “Acute Oral Toxicity-Acute Toxic Class Method”. Adopted on 17 December 2011

    Google Scholar 

  • Ojo OA, Oloyede O, Olarewaju O, Ojo AB, Ajiboye BO, Onikanni (2013) Studies of the crude aqueous leaves extracts of Ocimum gratissimum in albino rats. J Environ Sci Tox Food Tech 6:34–39

    Google Scholar 

  • Rajalakshmi A, Jayachitra A, Gopal P, Krithiga N (2014) Toxicity analysis of different medicinal plant extracts in Swiss albino mice. BioMed Res 1:1–6

    Google Scholar 

  • Senthilkumar R, Chandran R, Parimelazhagam (2014) Hepatoprotective effect of Rhodiola imbricate rhizome against paracetamol induced liver toxicity in rats. Saudi J Biol Sci 21:409–416

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shafi S, Tabassum N (2013) Phytochemical and acute toxicity study of Eriobotrya japonica seed extract in albino mice. Int J Res Pharm Biom Sci 4:202–205

    Google Scholar 

  • Singh A, Bhat TK, Sharma OP (2011) Clinical biochemistry of hepatotoxicity. J Clinic Toxicol S4:001

    Google Scholar 

  • Umadevi M, Kumar KPS, Bhowmik D, Duraivel S (2013) Traditionally used anticancer herbs in India. J Med Plan Stud 1:56–74

    Google Scholar 

  • Veeresham C (2012) Natural products derived from plants as a source of drugs. J Adv Pharm Technol Res 3:200–201

    Article  PubMed  PubMed Central  Google Scholar 

  • Walum E (1998) Acute oral toxicity. Environ Health Perspect 106:497–503

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors are thankful to the DBT MSUB major research project facility at the School of Biosciences, Mahatma Gandhi University, Kottayam for taking the photomicrographs of tissue sections.

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  1. School of Biosciences, Mahatma Gandhi University, P.D. Hills P.O, Kottayam, Kerala, 686560, India

    Lal Raisa Helen, Kuriakose Jayesh & M. S. Latha

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  1. Lal Raisa Helen
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  2. Kuriakose Jayesh
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  3. M. S. Latha
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Correspondence to M. S. Latha.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the Institution or practice at which the studies were conducted.

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Informed consent was obtained from all individual participants included in the study.

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Helen, L.R., Jayesh, K. & Latha, M.S. Safety assessment of aqueous acetone extract of Clerodendrum infortunatum L. in Wistar rats. Comp Clin Pathol 26, 369–375 (2017). https://doi.org/10.1007/s00580-016-2383-1

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