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Identification and morphologic and molecular characterization of Cyclospora macacae n. sp. from rhesus monkeys in China

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Abstract

Cyclospora spp. in nonhuman primates are most closely related to Cyclospora cayetanensis, an emerging human pathogen causing outbreaks of cyclosporiasis in North America. Studies thus far indicate the possible existence of host specificity in Cyclospora spp. In this study, 411 fecal specimens from free-range rhesus monkeys (Macaca mulatta) were collected and examined for Cyclospora by sequence analysis of the small subunit rRNA gene. A novel Cyclospora species was identified in 28 (6.8 %) specimens and named Cyclospora macacae based on morphologic and molecular characterizations. The oocyst of C. macacae is spherical and measures 8.49 ± 0.55 × 8.49 ± 0.49 μm in diameter. Phylogenetic analysis grouped this species together with the other four Cyclospora species infecting primates, including C. cayetanensis in humans, forming a monophyletic group closely related to avian Eimeria species. In addition, C. cayetanensis was detected in one specimen, although whether rhesus monkeys can serve as a natural reservoir host of C. cayetanensis needs further investigation.

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Acknowledgments

This work was supported in part by the National Natural Science Foundation of China (31425025 and 31302078), China Postdoctoral Science Foundation (2014 M560310), Open Funding Project of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou, China (SKLVEB2014KFKT008), and Fundamental Research Funds for the Central Universities, China. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, China

    Na Li, Jianbin Ye, Jingbo Ma, Lin Wang, Hailing Xu & Yaoyu Feng

  2. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

    Michael J. Arrowood & Lihua Xiao

Authors
  1. Na Li
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  2. Jianbin Ye
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  3. Michael J. Arrowood
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  4. Jingbo Ma
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  5. Lin Wang
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  6. Hailing Xu
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  7. Yaoyu Feng
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  8. Lihua Xiao
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Correspondence to Yaoyu Feng.

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Li, N., Ye, J., Arrowood, M.J. et al. Identification and morphologic and molecular characterization of Cyclospora macacae n. sp. from rhesus monkeys in China. Parasitol Res 114, 1811–1816 (2015). https://doi.org/10.1007/s00436-015-4367-5

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  • DOI: https://doi.org/10.1007/s00436-015-4367-5

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