Abstract
Intestinal capillariasis is an emerging helminthic zoonosis caused by Capillaria philippinensis and is frequently fatal if not diagnosed correctly. The present study demonstrates cross-reactivity between Trichinella spiralis larval antigens and C. philippinensis-infected human sera by immunoblotting. Sera from 16 proven intestinal capillariasis patients and 16 proven trichinosis patients were tested. The antigenic patterns recognized by intestinal capillariasis sera varied with the molecular masses, ranging from less than 20.1 to more than 94 kDa. The immunoblotting profiles of the trichinosis sera were similar to those of the intestinal capillariasis sera. The antigenic bands with 100% reactivity were located at 36.5, 40.5, and 54 kDa, respectively. Sera from patients with trichuriasis, strongyloidiasis, opisthorchiasis, and healthy controls differed clearly from the previous two and produced very faint patterns of reactivity and attenuated bands. This assay is potentially useful for large-scale screenings of persons at risk for C. philippinensis infection. Parasitological stool examinations of the positive cases are necessary as second-tier laboratory tests for confirming the diagnosis.
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References
Andrade SB, Andrade ZA (2004) Experimental hepatic fibrosis due to Capillaria hepatica infection (differential feature presented by rats and mice). Mem Inst Oswaldo Cruz 99:399–406
Aronstein WS, Lewis SA, Norden AP, Dalton JP, Strand M (1986) Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica. Parasitology 92:133–151
Austin DN, Mikhail MG, Chiodini PL, Murray-Lyon IM (1999) Intestinal capillariasis acquired in Egypt. Eur J Gastroenterol Hepatol 11:935–936
Banzon TC, Lewert RM, Yogore MG (1975) Serology of Capillaria philippinensis infection: reactivity of human sera to antigens prepared from Capillaria obsignata and other helminthes. Am J Trop Med Hyg 24:256–263
Beaver PC, Jung RC, Cupp EW (1984) Aphasmid nematodes. In: Beaver PC, Jung RC, Cupp EW (eds) Clinical parasitology, Lea & Febiger, Philadelphia, PA, pp 231–252
Bouchet F (1997) Intestinal capillariasis in neolithic inhabitants of Chalain (Jura, France). Lancet 349:256
Chapa-Ruiz MR, Salinas-Tobon MR, Aguilar-Alvarez DJ, Martinez-Maranon R (1992) Recognition of Trichinella spiralis muscle larvae antigens by sera from human infected with this parasite and its potential use in diagnosis. Rev Latinoam Microbiol 34:95–99
Chen CY, Hsieh WC, Lin JT, Liu MC (1989) Intestinal capillariasis: report of a case. Taiwan Yi Xue Hui Za Zhi 88:617–620
Chichino G, Bernuzzi AM, Bruno A, Cevini C, Atzori C, Malfitano A, Scaglia M (1992) Intestinal capillariasis (Capillaria philippinensis) acquired in Indonesia: a case report. Am J Trop Med Hyg 47:10–12
Chitwood MB, Valesquez C, Salazar NG (1968) Capillaria philippinensis sp. n. (Nematoda: Trichinellida), from the intestine of man in the Philippines. J Parasitol 54:368–371
Cross JH (1992) Intestinal capillariasis. Clin Microbiol Rev 5:120–129
De-la-Rosa JL, Alcantara P, Correa D (1995) Investigation of cross-reactions against Trichinella spiralis antigens by enzyme-linked immunosorbent assay and enzyme-linked immunoelectrotransfer blot assay in patients with various diseases. Clin Diagn Lab Immunol 2:122–124
Dronda F, Chaves F, Sanz A, Lopez-Velez R (1993) Human intestinal capillariasis in an area of nonendemicity: case report and review. Clin Infect Dis 17:909–912
El Dib NA, Sabry MA, Ahmed JA, El-Basiouni SO, El-Badry AA (2004) Evaluation of Capillaria philippinensis coproantigen in the diagnosis of infection. J Egypt Soc Parasitol 34:97–106
Erdman DD (1981) Clinical comparison of ethyl acetate and diethyl ether in the formalin–ether sedimentation technique. J Clin Microbiol 4:483–485
Hoghooghi-Rad N, Maraghi S, Narenj-Zadeh A (1987) Capillaria philippinensis infection in Khoozestan Province, Iran: case report. Am J Trop Med Hyg 37:135–137
Justus DE, Morakote N (1981) Mast cell degranulation associated with sequestration and removal of Trichinella spiralis antigens. Int Arch Allergy Appl Immunol 64:371–384
Kang G, Mathan M, Ramakrishna BS, Mathai E, Sarada V (1994) Human intestinal capillariasis: first report from India. Trans R Soc Trop Med Hyg 88:204
Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Mahannop P, Setasuban P, Morakote N, Tapchaisri P, Chaicumpa W (1995) Immunodiagnosis of human trichinellosis and identification of specific antigen for Trichinella spiralis. Int J Parasitol 25:87–94
McCarthy J, Moore TA (2000) Emerging helminth zoonoses. Int J Parasitol 30:1351–1360
Morakote N, Sukhavat K, Khamboonruang C, Siriprasert V, Suphawitayanukul S, Thamasonthi W (1992) Persistence of IgG, IgM, and IgE antibodies in human trichinosis. Trop Med Parasitol 43:167–169
Nunez GG, Malmassari SL, Costantino SN, Venturiello SM (2000) Immunoelectrotransfer blot assay in acute and chronic human trichinellosis. J Parasitol 86:1121–1124
Pozio E, Khamboonruang C (1989) Trichinellosis in Thailand: epidemiology and biochemical identification of the aethiological agent. Trop Med Parasitol 40:73–74
Pradatsundarasar A, Pecharanond K, Chintanawongs C, Ungthavorn P (1973) The first case of intestinal capillariasis in Thailand. Southeast Asian J Trop Med Public Health 4:131–134
Roach TI, Wakelin D, Else KJ, Bundy DA (1988) Antigenic cross-reactivity between the human whipworm, Trichuris trichiura, and the mouse trichuroids Trichuris muris and Trichinella spiralis. Parasite Immunol 10:279–291
Robert F, Weil B, Kassis N, Dupouy-Camet J (1996) Investigation of immunofluorescence cross-reactions against Trichinella spiralis by Western blot (immunoblot) analysis. Clin Diagn Lab Immunol 3:575–577
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Acknowledgements
This investigation was supported by a grant from the Khon Kaen University. We thank Dr. Mark Roselieb for improving the English language presentation of the manuscript. The use of experimental animals complies with the current laws of Thailand wherein the experiment was performed.
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Intapan, P.M., Maleewong, W., Sukeepaisarnjaroen, W. et al. Potential use of Trichinella spiralis antigen for serodiagnosis of human capillariasis philippinensis by immunoblot analysis. Parasitol Res 98, 227–231 (2006). https://doi.org/10.1007/s00436-005-0070-2
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DOI: https://doi.org/10.1007/s00436-005-0070-2