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Molecular and histological evaluation of tumor necrosis factor-alpha expression in Helicobacter pylori-mediated gastric carcinogenesis

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Abstract

Purpose

Helicobacter pylori (H. pylori) is considered to be a major factor contributing to gastric mucosal damage by stimulating mucosal macrophage production of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), but the inflammatory responses within the gastric mucosa in vivo are not well known. Therefore, this study was designed to investigate the expression of TNF-α induced by H. pylori infection which is involved in the tumor initiation and promotion of gastric carcinogenesis.

Methods

This study was carried out in 200 patients, consisting of normal gastric mucosa (n = 20), mucosa with chronic gastritis (n = 63), intestinal metaplasia (n = 20), dysplasia (n = 11), and gastric adenocarcinoma (n = 86), in which the H. pylori status has been analyzed. The expression of TNF-α was studied at mRNA as well as protein level using RT-PCR and western blotting, respectively. The localization of TNF-α was also studied semiquantitatively by immunohistochemistry.

Results

The RT-PCR and western blotting results of TNF-α mRNA and protein expressions were significantly increased in chronic gastritis, intestinal metaplasia, dysplasia and gastric adenocarcinoma patients, respectively. Immunohistochemical study also showed the increased expression of TNF-α in the similar way.

Conclusion

Over expression of TNF-α showed a significant severity-dose–response as risk markers from preneoplastic lesions to gastric cancer.

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Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Unit of Biochemistry, Department of Zoology, School of Life Sciences, University of Madras, Guindy Campus, Chennai, 600 025, Tamil Nadu, India

    Cinghu Senthilkumar & Halagowder Devaraj

  2. Department of Radiation Oncology, College of Medicine, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheongju, Chungbuk, 361-763, Korea

    Cinghu Senthilkumar

  3. Department of Biochemistry & Molecular Biology, University of Madras, Guindy Campus, Chennai, 600 025, India

    Sivasithambaram Niranjali

  4. Department of Medical Gastroenterology, Stanly Medical College and Hospital, Chennai, 600 001, India

    Venkatraman Jayanthi

  5. Department of Applied Biochemistry, Division of Life Science, College of Biomedical and Health Science, Konkuk University, Chungju, 380-701, Korea

    Thiyagarajan Ramesh

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  1. Cinghu Senthilkumar
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  2. Sivasithambaram Niranjali
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  3. Venkatraman Jayanthi
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  4. Thiyagarajan Ramesh
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  5. Halagowder Devaraj
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Correspondence to Thiyagarajan Ramesh or Halagowder Devaraj.

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Senthilkumar, C., Niranjali, S., Jayanthi, V. et al. Molecular and histological evaluation of tumor necrosis factor-alpha expression in Helicobacter pylori-mediated gastric carcinogenesis. J Cancer Res Clin Oncol 137, 577–583 (2011). https://doi.org/10.1007/s00432-010-0921-9

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  • DOI: https://doi.org/10.1007/s00432-010-0921-9

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